Overexpressed CYP450 mediated apoptosis evaluates cytotoxicity and teratotoxicity of Cucumis callosus

Panda, Siva Prasad; Jena, Bikash Ranjan; Kalyani, Gade; Panigrahy, Uttam Prasad

doi:10.1007/s13596-018-0324-z

Cited by 3 publications

(1 citation statement)

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“…The structure of TMF was characterised using 1D and 2D NMR spectroscopy and compared with available data from the literature and respective research. [ 28 ]…”

Section: Methodsmentioning

confidence: 99%

A trimethoxy flavonoid isolated from stem extract of Tabebuia chrysantha suppresses angiogenesis in angiosarcoma

Panda

Panigrahy

Prasanth

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives This research aimed to evaluate the antiangiogenic activity of isolated flavonoid 4a,5,8,8a-tetrahydro-5-hydroxy-3,7,8-trimethoxy-2-(3,4-dimethoxyphenyl) chromen-4-one (TMF) from Tabebuia chrysantha. STAT3-MMP9 signalling is a signal transduction mechanism that promotes angiogenesis in various cancers. Methods The tumour xenografting chicken embryo chorioallantoic membrane (CAM) model-based ex vivo assay was used to evaluate the activity of TMF. The Western blot, densitometric analysis and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the activity of the MMP9. Zebrafish embryos were used to evaluate embryotoxicity, and in vitro free radical scavenging activity of flavonoid was also elucidated. Key findings This research assessed the high level of STAT3, p-ERK, VEGF-R and MMP9 in the tissue extract of the control group, and also, the suppression of angiogenesis in the treatment groups was due to scavenged ROS and RNS, dephosphorylation of STAT3 and ERK, and suppression of MMP9 gene expression. Conclusion The isolated flavonoid named TMF from T. chrysantha functions as specific regulators of target proteins of angiosarcoma. The STAT3-MMP9 signalling may be used as an effective prognostic marker of angiosarcoma.

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“…The structure of TMF was characterised using 1D and 2D NMR spectroscopy and compared with available data from the literature and respective research. [ 28 ]…”

Section: Methodsmentioning

confidence: 99%