Overexpression of miR-26a-2 in human liposarcoma is correlated with poor patient survival

Lee, D H; Amanat, Soroosh; Goff, Catherine; Weiss, Lawrence M.; Said, Jonathan; Doan, Ngan; Sato‐Otsubo, Aiko; Ogawa, Seishi; Forscher, Charles; Koeffler, H. Phillip

doi:10.1038/oncsis.2013.10

Cited by 47 publications

(49 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…11 In a copy number variation study, miR-26a-2 has been found to be amplified and highly expressed in 22 MLS patients and affects cell proliferation and survival by inhibiting a regulator of chromosome condensation and BTB domain containing protein 1. 12 Global miRNA expression analyses using microarrays from high-grade soft tissue sarcomas 13 and various subtypes of LPS 14 have enabled the identification and classification of MLS tumors based on their miRNA expression profiles.…”

Section: Introductionmentioning

confidence: 99%

miR-135b, a key regulator of malignancy, is linked to poor prognosis in human myxoid liposarcoma

Nezu

Hagiwara²,

Yamamoto³

et al. 2016

Oncogene

View full text Add to dashboard Cite

Myxoid/round cell (RC) liposarcomas (MLS) were originally classified into two distinct populations based on histological differences; a myxoid component and a RC component. It is notable that, depending on an increase of the RC component, the prognosis significantly differs. Hence, the RC component is associated with metastasis and poor prognosis. However, the molecular mechanisms that contribute to the malignancy of the RC component still remain largely unknown. Here, we report microRNA-135b (miR-135b), a key regulator of the malignancy, highly expressed in the RC component and promoting MLS cell invasion in vitro and metastasis in vivo through the direct suppression of thrombospondin 2 (THBS2). Decreased THBS2 expression by miR-135b increases the total amount of matrix metalloproteinase 2 (MMP2) and influences cellular density and an extracellular matrix structure, thereby resulting in morphological change in tumor. The expression levels of miR-135b and THBS2 significantly correlated with a poor prognosis in MLS patients. Overall, our study reveals that the miR-135b/THBS2/MMP2 axis is tightly related to MLS pathophysiology and has an important clinical implication. This work provides noteworthy evidence for overcoming metastasis and improving patient outcomes, and sheds light on miR-135b and THBS2 as novel molecular targets for diagnosis and therapy in MLS.

show abstract

Section: Introductionmentioning

confidence: 99%

miR-135b, a key regulator of malignancy, is linked to poor prognosis in human myxoid liposarcoma

Nezu

Hagiwara²,

Yamamoto³

et al. 2016

Oncogene

View full text Add to dashboard Cite

show abstract

“…Previously, tumor suppressive miR-26a has been studied in numerous types of human cancer, and it was revealed to serve a key role as a cancer suppressor gene in liposarcoma (21), thyroid carcinoma (22) and breast cancer (23). miR-26a was demonstrated to be upregulated in glioma, and may enhance cancer cell growth and colony formation (24).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

et al. 2018

View full text Add to dashboard Cite

Abstract. The aim of the present study was to investigate the expression and role of microRNA-26a (miR-26a) in lung cancer, and to verify whether differentially expressed in adenocarcinoma of the lung (DAL-1) is the target protein of miR-26a. mRNA expression levels of miR-26a and DAL-1 were detected using reverse transcription-quantitative polymerase chain reaction. Protein expression levels of DAL-1 and annexin A1 (ANXA1) were evaluated by western blot analysis. Cell Counting Kit-8, Transwell and wound scratch healing assays were used to characterize the function of miR-26a in lung cancer cells. The association of DAL-1 with miR-26a or ANXA1 was determined by dual-luciferase reporter or two-dimensional gel electrophoresis assays. miR-26a revealed decreased expression levels in lung cancer tissues compared with normal lung tissues, and decreased expression levels in lung cancer cells compared with 16HBE cells. Inhibition of miR-26a promoted lung cancer cell growth, migration and invasion. The DAL-1 protein exhibited downregulated expression levels in lung cancer tissues. DAL-1 was not the direct target gene of miR-26a. The two-dimensional gel electrophoresis assay confirmed that DAL-1 and ANXA1 were associated proteins. Expression levels of the ANXA1 protein were increased following DAL-1 gene silencing. The altered expression level of miR-26a affected the expression of ANXA1, and not of DAL-1. miR-26a demonstrated decreased expression levels in lung cancer cells, and it has an important effect on the biological function of lung cancer cells. However, DAL-1 was not a target gene of miR-26a. As a DAL-1 associated protein, ANXA1 was regulated by miR-26a. IntroductionLung cancer has a high incidence of tumor recurrence and metastasis, and is the most common cause of cancer-associated morality worldwide (1). The overall 5-year survival rate among patients with lung cancer is <15%, and a high rate of metastasis is the primary cause of lung cancer-associated mortality (2). A previous study confirmed that differentially expressed in adenocarcinoma of the lung (DAL-1), a protein that belongs to the membrane-associated cytoskeleton protein 4.1 family, is an efficient suppressor of epithelial-mesenchymal transition (EMT) in lung cancer (3). EMT is a pivotal event in lung cancer metastasis progression (4-6). However, the regulators of DAL-1 in EMT remain unknown.Recently, a novel batch of endogenous small non-coding regulatory RNAs (microRNA's; miRNA's) have received attention in the development of cancer, miRNA's bind complementary sequences in target mRNAs, resulting in selective degradation or selective inhibition of their translation (7). The present study investigated the possible miRNAs of DAL-1 using bioinformatic assays, which included miR-26b, miR-26a, miR-96 and miR-223. Among them, the regulation of DAL-1 by miR-223 has been demonstrated to serve a role in gastric cancer (8). The present study revealed that the gene silencing of DAL-1 induced annexin A1 (ANXA1) protein expression levels to increase in H460 cells...

show abstract

“…[20][21][22][23] For example, cytogenomic array studies (aCGH or SNP array) have identified recurrent patterns of copy number changes and/or cnLOH in embryonal rhabdomyosarcoma ( þ 2, þ 7, þ 8, þ 11, þ 12, þ 13, þ 20, cnLOH 11p15.5) with acquisition of genomic amplification in lesions distinguished by the presence of anaplasia, benign metastasizing leiomyoma (loss of 1p, 13q, 19q, and 22q material), and dedifferentiated liposarcoma whereby gain of amplicons in 1p32 and 6q23-25 (containing genes involved in the c-jun NH 2 -terminal kinase/mitogen-activated protein kinase pathway) parallels the progression from an atypical lipomatous tumor/well-differentiated liposarcoma to dedifferentiated liposarcoma, Figure 2. [24][25][26][27][28][29][30] …”

Section: Molecular Cytogenetic Analysismentioning

confidence: 99%

“…Dedifferentiated liposarcoma differs by the acquisition of complex secondary chromosomal changes representing coamplifications of other regions/genes such as 1p32 (JUN), 6q23 (ASK1), and 6q25 (MAP3K7IP2). 25,[28][29][30] For clinical purposes, molecular demonstration of MDM2 ( þ CDK4) amplification is recommended when the diagnosis of ALT/WDL or dedifferentiated liposarcoma is not possible based on clinicohistopathologic information alone.…”

Section: Loss Of Immunophenotype or Dedifferentiationmentioning

confidence: 99%

The role of cytogenetics and molecular diagnostics in the diagnosis of soft-tissue tumors

Bridge

2014

Modern Pathology

View full text Add to dashboard Cite

Overexpression of miR-26a-2 in human liposarcoma is correlated with poor patient survival

Cited by 47 publications

References 24 publications

miR-135b, a key regulator of malignancy, is linked to poor prognosis in human myxoid liposarcoma

miR-135b, a key regulator of malignancy, is linked to poor prognosis in human myxoid liposarcoma

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

The role of cytogenetics and molecular diagnostics in the diagnosis of soft-tissue tumors

Contact Info

Product

Resources

About