Overexpression of the A4-rolB gene from the pRiA4 of Rhizobium rhizogenes modulates hormones homeostasis and leads to an increase of flavonoid accumulation and drought tolerance in Arabidopsis thaliana transgenic plants

Rhizobium rhizogenes rolC gene promotes leaf senescence and enhances osmotic stress resistance in Arabidopsis: the positive role of abscisic acid

“…(Bulgakov et al 2013). Based on the present and previous studies on the view that rol genes of R. rhizogenes are able to enhance stress resistance in plants (Bulgakov et al 2013, Veremeichik et al 2022), we propose that the attack of plants by R. rhizogenes does not render them lethal, rather, it could subsequently induce resistant mechanisms improving plants' ability to survive in adverse environments. Therefore, the enhanced resistance to abiotic stress could be the benefit that plants derive from their symbiotic pattern with R. rhizogenes .…”

“…More importantly, rol C‐OX plants also exhibited enhanced resistance to osmotic stress during early growth (Figure 5A). In view of these features, we speculate that rol C gene could be involved in this ASE strategy, which enables rol C not only to activate metabolic processes and promote the accumulation of defense‐related secondary metabolites in plant tissues, such as phytoalexin (Chandra 2012, Matveeva et al 2015), but also to enhance stress resistance in plants (Veremeichik et al 2022) and rol C‐expressing cell cultures (Victor P. Bulgakov 2008, Shkryl et al 2022). As one of the metabolites altered by the call for premature senescence, ABA has been shown to be regulated by the rol C gene in this study (Figures 3‐5), thus it might serve as a key conjunction mediator between early senescence and resistance enhancement in rol C‐OX plants.…”

“…Secondly, the interest has also been focused on plant metabolic engineering since R. rhizogenes and single rol genes are powerful inducers of bioactive molecules, such as secondary metabolites and recombinant proteins (Bulgakov 2008, Shkryl et al 2008, Bulgakov et al 2013, Martínez et al 2020). However, increased attention has been recently paid to the potential use of R. rhizogenes strains or single rol genes to confer stress resilience to plants, e.g., drought resistance (Arshad et al 2014, Bulgakov et al 2018, Veremeichik et al 2022).…”

Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene

“…In the present study, we used a plant clone called AtB-1, which is a cloned variant of the A4-rolB-transformed B5 line [9]. The phenotype of the B5 line was described in detail in a recent article by Veremeichik et al [9] and is similar to the previously described phenotype of Arabidopsis plants transformed with the rolB gene [32]. The AtB-1 line does not show signs of necrosis or dwarfism and thus is suitable for investigating the effects of rolB.…”

“…These traits are associated with the high resistance of rolB-transformed cells to the ROS-inducing herbicides [7]. It was recently shown that rolB confers tolerance of Arabidopsis plants to drought stress [9]. Bettini et al [10,11] showed the role of rolB in photosynthesis, demonstrating significantly increased non-photochemical quenching in rolB-transformed tomato plants.…”