Overlapping Divergent Transcripts Mapping to the HindIII F Region of the Autographa Californica Nuclear Polyhedrosis Virus

Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome

“…They were all most abundant at 18 h p.i. The 2-1 kb transcript matches the size of the pair of leftward transcripts mapped by Hardin & Weaver (1990). The other two were not previously observed, presumably because the probe used by Hardin & Weaver did not extend to the left of the XhoI site.…”

“…The deduced amino acid sequences of the three open reading frames, ORF-I, ORF-2 and ORF-3 are indicated. The 5' ends of the ES3 transcript described in this report and those of ES1 and ES2 reported earlier (Hardin & Weaver, 1990) are indicated by arrows. Potential polyadenylation signals are overlined.…”

“…One of these encodes a polypeptide of 151 and the other one of 329 amino acids. These ORFs are contained within the leftward transcript mapped by Hardin & Weaver (1990), and the larger ORF lies within the new leftward transcript mapped here. A third ORF mapping to the opposite strand encodes a potential polypeptide of 167 amino acids.…”

“…Expression of the viral genes is temporally regulated, and transcripts have been categorized as immediate early, delayed early, late and very late (Friesen & Miller, 1986). Transcriptional mapping of the viral genome has revealed both unidirectional (Friesen & Miller, 1985;Rankin et al, 1986;Oellig et al, 1987) and bidirectional Hardin & Weaver, 1990) overlapping transcription in several segments of the genome. We previously demonstrated a pair of oppositely oriented transcripts within the HindlII F region of the AcNPV genome (Hardin & Weaver, 1990).…”

“…Transcriptional mapping of the viral genome has revealed both unidirectional (Friesen & Miller, 1985;Rankin et al, 1986;Oellig et al, 1987) and bidirectional Hardin & Weaver, 1990) overlapping transcription in several segments of the genome. We previously demonstrated a pair of oppositely oriented transcripts within the HindlII F region of the AcNPV genome (Hardin & Weaver, 1990). These transcripts cross the unique XhoI restriction site located in this region and overlap in a region of about 550 nucleotides (nt) at their 5' ends.…”