Oxidation of Thiosulphate by Thiobacillus thioparus

Skarzynski, B; Szczepkowski, T

doi:10.1038/1831413a0

Cited by 20 publications

(9 citation statements)

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“…Skarzynski & Szczepkowski (1959) suggested that polythionates were not concerned, and that the sulphur passed through organic intermediates. Santer et al (1960) also suggested the existence of an organic intermediate, and Trudinger (1959) showed the incorporation of radioactive sulphur into an unknown compound after its incorporation into tetrathionate and trithionate.…”

Section: Discussionmentioning

confidence: 99%

“…trautweinii, which he considered was a heterotrophic organism and which has since been reported to be facultatively autotrophic (Parker & Prisk, 1953). Skarzynski & Szczepkowski (1959), by using a chromatographic method, were unable to detect the presence of polythionates in cultures of T . thioparus; they concluded that polythionates were not concerned in thiosulphate oxidation.…”

Section: Introductionmentioning

confidence: 99%

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The Occurrence of Polythionates as Intermediates in the Metabolism of Thiosulphate by the Thiobacilli

Jones¹,

Happold²

1961

Journal of General Microbiology

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SUMMARYFurther evidence for the inclusion of polythionates in the pathway of thiosulphate oxidation by members of the genus Thiobacillus is presented. Manometric experiments showed tetrathionate oxidation to be a stage in thiosulphate oxidation by suspensions of T . thioparus and T. thiocyanoxidans. Thiobltcillus thioparus accumulated a sufficient concentration of polythionate in the medium to allow chromatograms to be prepared, and the type of polythionate accumulated was influenced by the ratio between the sodium and potassium ions in the medium. The restriction of the oxidation of tetrathionate at the lower concentrations of K+ may be due to a restriction in the entry of phosphate into the cell during growth; this is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Occurrence of Polythionates as Intermediates in the Metabolism of Thiosulphate by the Thiobacilli

Jones¹,

Happold²

1961

Journal of General Microbiology

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“…Thiosulphate and polythionates were separated by descending chromatography on Whatman no. 1 paper with the solvent systems described by Skarzynski & Szczepkowski (1959) and Trudinger (1959). The chromatograms were dried a t room temperature and the sulphur compounds located by the method of Trevelyan (1950).…”

Section: Methodsmentioning

confidence: 99%

The Role of Tetrathionate in the Oxidation of Thiosulphate by Chromatium sp. strain D

Smith¹

1966

Journal of General Microbiology

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SUMMARYSulphate was progressively replaced by tetrathionate as the end product of thiosulphate oxidation by suspensions of Chromatium D when the pH value was decreased from 7-3 to 6-25; tetrathionate was not itself oxidized to sulphate within this range. The effect of pH value on the oxidation of endogenous sulphur was less marked than on the production of sulphate from thiosulphate. Extracts of Chromatium D catalysed the oxidation of thiosulphate to tetrathionate in the presence of ferricyanide; pH 5.0 was the optimum of the purified enzyme. Tetrathionate inhibited growth and the complete oxidation of thiosulphate by suspensions. At pH 6-75 where sulphur accumulated during thiosulphate utilization and sulphate and tetrathionate were formed, added tetrathionate inhibited the accumulation of endogenous sulphur but not the production of tetrathionate from thiosulphate. Tetrathionate had no effect on the oxidation of endogenous sulphur by suspensions. It is concluded that in Chromatium D thiosulphate is metabolized by two alternative pathways depending on the conditions : (1) the cleavage of one molecule of thiosulphate in a reaction similar to that catalysed by thiosulphate sulphur-transferase E. (2.2.8 .I. 1. (rhodanese); (2) the oxidative coupling of two molecules of thiosulphate to give tetrathionate, catalysed by the thiosulphate-oxidizing enzyme which only operates at low pH values.

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“…The purity of the thionates was checked by the method of Jay (3) and by paper chromatography (20). The paper chromatography method also was employed to quantitate the thionates formed by the thiosulfate-catalyzed breakdown of tetrathionate.…”

Section: Methodsmentioning

confidence: 99%