Oxidative DNA damage in mouse sperm chromosomes: Size matters

Kocer, Ayhan; Henry-Berger, Joëlle; Noblanc, Anaïs; Champroux, Alexandre; Pogorelcnik, Romain; Guiton, Rachel; Janny, L.; Pons-Rejraji, Hanae; Saez, Fabrice; Johnson, Graham D.; Krawetz, Stephen A.; Álvarez, Juan G.; Aitken, R. John; Drevet, Joël R.

doi:10.1016/j.freeradbiomed.2015.10.419

Cited by 31 publications

(65 citation statements)

References 44 publications

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“…In mice and human spermatozoa, immunocytochemical approaches reveal localized histones at the periphery of the nucleus as well as in the post-acrosomal and basal domains of the sperm head [93–95]. The basal localization of the histone signal resembles that of the nuclear annulus [75].…”

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

“…This structure is seen as a component of the sperm nuclear matrix, acting as an anchor for the sperm DNA via the sperm MARs (localized among toroids) and the histone-rich telomeres [94, 96]. In mouse sperm we recently reported that nuclear domains rich in matrix proteins are also histone-rich regions of lower compaction [95, 97]. …”

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

“…Ward [98] suggested that these segments of DNA are organized in a less condensed state with a solenoid-like conformation resembling the somatic chromatin. Additionally, high histone levels were found in interspersed short DNA sequences (around 1 kb-long) that correspond to the small DNA linkers bridging toroids together [95, 97, 98]. Interestingly, the latter regions were also reported to be associated with the sperm nuclear matrix [95, 97, 98].…”

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

“…Additionally, high histone levels were found in interspersed short DNA sequences (around 1 kb-long) that correspond to the small DNA linkers bridging toroids together [95, 97, 98]. Interestingly, the latter regions were also reported to be associated with the sperm nuclear matrix [95, 97, 98]. ChIP studies utilizing histone antibodies to recover the histone-associated DNA sequences in mature sperm revealed histones to be significantly enriched at the promoters of: genes coding for microRNAs, genes involved in early embryonic development (e.g.…”

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

“…transcription factors, HOX genes, signaling protein, etc.…), genes subjected to genomic imprinting, and genes involved in spermatogenesis [99]. However, conflicting data from different groups showed that persisting histones were associated with intergenic sequences outside of the gene regulatory regions [95, 100]. To date, there is no consensus as to what specific DNA sequences in sperm are associated with nucleosomes.…”

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

See 4 more Smart Citations

Mammalian sperm nuclear organization: resiliencies and vulnerabilities

Champroux

Torres-Carreira

Gharagozloo

et al. 2016

Basic Clin. Androl.

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Sperm cells are remarkably complex and highly specialized compared to somatic cells. Their function is to deliver to the oocyte the paternal genomic blueprint along with a pool of proteins and RNAs so a new generation can begin. Reproductive success, including optimal embryonic development and healthy offspring, greatly depends on the integrity of the sperm chromatin structure. It is now well documented that DNA damage in sperm is linked to reproductive failures both in natural and assisted conception (Assisted Reproductive Technologies [ART]). This manuscript reviews recent important findings concerning - the unusual organization of mammalian sperm chromatin and its impact on reproductive success when modified. This review is focused on sperm chromatin damage and their impact on embryonic development and transgenerational inheritance.

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Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

Section: The Male Germinal Chromatin: a Unique And Elaborate Structurementioning

confidence: 99%

See 3 more Smart Citations

Mammalian sperm nuclear organization: resiliencies and vulnerabilities

Champroux

Torres-Carreira

Gharagozloo

et al. 2016

Basic Clin. Androl.

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The potential for nanomaterial toxicity affecting the male reproductive system

Ning

Zhang

et al. 2022

WIREs Nanomed Nanobiotechnol

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With the development of nanotechnology, nanomaterials offer great advantages in a wide variety of industrial and consumer products, and show promise for biomedical applications. However, with these new products, nanomaterial pollutants may enter the human body to cause adverse health effects, including hazards to the male reproductive system. Nanomaterials can enter the body through inhalation, oral exposure, or intravenous injection, and reach the testis via the blood, penetrate the Sertoli cell barrier, and directly or indirectly elicit toxicopathological changes to the testicles. These may then trigger hormone disorders, inhibit spermatogenic cell proliferation, and induce apoptosis, ultimately leading to a decrease in sperm motility and number, ultimately diminishing male reproductive capacity. This review will discuss the toxicological effects of nanomaterials on the male reproductive system, including inflammation, the impact on the hypothalamic–pituitary–gonadal axis (HPG axis), lipid peroxidation, and free ion release relevant to germ cells, Sertoli cell tight junctions, and the gonadal endocrine system.This article is categorized under: Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials

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