Oxidative DNA damage is involved in cigarette smoke-induced lung injury in rats

Chen, Zhihai; Wang, Dapeng; Xing, Liudong; Pei, Weiwei; Li, Jianxiang; Cao, Yi; Zhang, Jie; An, Yan; Nie, Jing; Tong, Jian

doi:10.1007/s12199-015-0469-z

Cited by 51 publications

(30 citation statements)

References 35 publications

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“…It is well known that cigarette smoke contains large amounts of reactive oxygen species (ROS) and ROS-induced oxidative DNA damage has been reported to play a major role in lung cancer (Chen et al, 2015). Tobacco-specific nitrosamines have been reported to be potent clastogenic and mutagenic agents that are thought to be responsible for the induction of chromatid/ chromosomal aberrations resulting in the production of micronuclei (MNi) (Kumar et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The Micronuclei Scoring as a Biomarker for Early Detection of Genotoxic Effect of Cigarette Smoking

Mohammed

Hussen

Rashad

2020

Asian Pac J Cancer Prev

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Objective: The main aim of this study is to evaluate the micronuclei scoring as a biomarker for early detection and screening of genotoxic effect of cigarette smoking in the peripheral blood T-lymphocytes. Methods: A total number of eligible 148 individuals have participated in the study; 78 Current smokers and 70 never smokers. Cytokinesis-block micronucleus assay was performed for all the participants in the peripheral blood T-lymphocytes. Assessment of the smoking status of the participants was conducted through the detailed smoking history, Fagerström test for nicotine dependence (FTND) scoring, and determination of the urinary cotinine creatinine ratio (CCR). Result: A significantly higher frequency of micronuclei in the binucleated T-lymphocytes(BMNi) was identified in the smokers group as compared to the nonsmokers; OR=4.9, 95% CI=1.9-12.5), P-value=0.006. Both of the pack years and the smoking duration of the smokers could significantly predict the BMNi scoring; P-value=0.001, 0.002 respectively. Conclusion: Our results indicate the association between BMNi and cigarette smoking, suggesting that BMNi Scoring can be a useful biomarker for early detection and screening of the genotoxic effect of cigarette smoking as a primary preventive measure for various smoking induced cancers.

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Section: Discussionmentioning

confidence: 99%

The Micronuclei Scoring as a Biomarker for Early Detection of Genotoxic Effect of Cigarette Smoking

Mohammed

Hussen

Rashad

2020

Asian Pac J Cancer Prev

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“…At least some of the impairment of ER homeostasis caused by cigarette smoke is mediated through effects on cellular redox status. Cigarette smoke contains many potent oxidizing agents and so the lungs of humans and mice exposed to smoke have elevated levels of oxidized macromolecules [46,100]. Similar biochemical changes are observed when human airway epithelial cells are treated with smoke condensate in vitro [101,102].…”

Section: The Role Of Redox In Smoke-induced Er Stressmentioning

confidence: 98%

Pulmonary endoplasmic reticulum stress—scars, smoke, and suffocation

et al. 2018

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Protein misfolding within the endoplasmic reticulum (ER stress) can be a cause or consequence of pulmonary disease. Mutation of proteins restricted to the alveolar type II pneumocyte can lead to inherited forms of pulmonary fibrosis, but even sporadic cases of pulmonary fibrosis appear to be strongly associated with activation of the unfolded protein response and/or the integrated stress response. Inhalation of smoke can impair protein folding and may be an important cause of pulmonary ER stress. Similarly, tissue hypoxia can lead to impaired protein homeostasis (proteostasis). But the mechanisms linking smoke and hypoxia to ER stress are only partially understood. In this review, we will examine the role of ER stress in the pathogenesis of lung disease by focusing on fibrosis, smoke, and hypoxia.

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“…Asap rokok dengan kandungan sejumlah besar radikal bebas, mampu memulai atau mempromosi kerusakan oksidatif pada level seluler. Kerusakan oksidatif pada tingkat DNA juga telah diamati setelah pemaparan asap rokok (4).…”

Section: Pendahuluanunclassified

EFEK PEMBERIAN EKSTRAK ETANOL RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe) TERHADAP PERUBAHAN KADAR PROTEIN TOTAL DAN ALKALI FOSFATASE PADA TIKUS (Rattus norvegicus) YANG DIPAPARKAN ASAP ROKOK

Amin

Yanti

Sartini

et al. 2019

MFF

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Temu putih (Curcuma zedoaria (Berg.) Roscoe) telah banyak digunakan secara tradisional sebagai pengobatan penyakit. Temu putih diketahui memiliki aktivitas antioksidan yang berpotensi memperbaiki kerusakan sel. Tujuan penelitian ini yaitu mengevaluasi efek ekstrak temu putih (ETP) melalui perubahan kadar protein total dan alkali fosfatase (ALP) pada tikus yang dipaparkan asap rokok. Sebanyak 15 ekor tikus dibagi menjadi 5 kelompok (n=3) yaitu kelompok kontrol negatif, kontrol positif, kelompok induksi asap rokok dan ETP 70 mg/200gBB, kelompok induksi asap rokok dan ETP 105 mg/200gBB, dan kelompok pemberian ETP 105 mg/200gBB. Perlakuan dilakukan selama 30 hari. Pada hari ke-0 dan hari ke-30, diambil darah tikus untuk mengevaluasi perubahan kadar protein total dan ALP. Hasil penelitian menunjukkan pemberian ETP secara peroral pada hewan coba tikus yang telah dipaparkan asap rokok menurunkan kadar protein total secara signifikan dengan nilai p=0,038 dan 0,004 (p<0,05) masing-masing setelah pemberian ETP dosis 70 mg/200gBB dan 105 mg/200gBB. Pemberian dosis yang sama juga menunjukkan signifikansi penurunan kadar ALP dengan nilai p= 0,003 dan 0,001 (p<0,05) pada tikus yang terpapar asap rokok. Kesimpulan penelitan ini adalah ekstrak temu putih 70 mg/200gBB dan 105 mg/200gBB dapat menurunkan kadar protein total dan ALP tikus yang terpapar asap rokok selama 30 hari.

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Oxidative DNA damage is involved in cigarette smoke-induced lung injury in rats

Cited by 51 publications

References 35 publications

The Micronuclei Scoring as a Biomarker for Early Detection of Genotoxic Effect of Cigarette Smoking

The Micronuclei Scoring as a Biomarker for Early Detection of Genotoxic Effect of Cigarette Smoking

Pulmonary endoplasmic reticulum stress—scars, smoke, and suffocation

EFEK PEMBERIAN EKSTRAK ETANOL RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe) TERHADAP PERUBAHAN KADAR PROTEIN TOTAL DAN ALKALI FOSFATASE PADA TIKUS (Rattus norvegicus) YANG DIPAPARKAN ASAP ROKOK

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