Oxidative free radicals scavenging activity (in vitro and in vivo assay) of standardized fractions from the seeds of Argyreia speciosa (Ghav-patta) a traditional Indian medicine

Azmi, Lubna; Shukla, Ila; Goutam, Arti; Rao, Ch. V.; Jawaid, Talha; Awaad, Amani S.; Alqasoumi, Saleh I.; Alkhamees, Osama A.; Kamal, Mehnaz

doi:10.1016/j.jsps.2019.10.004

Cited by 10 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arthritis occurs in all age groups and peaks between the ages of 35 and 50, affecting ~1% of the world's population . Rheumatoid arthritis (RA) is a systemic inflammatory disease that attacks the joints by producing proliferative synovitis, leading to the destruction of articular cartilage and underlying bone . RA affects approximately 0.5–1% adults worldwide, with women being affected two to three times more frequently than men; RA is also associated with a high mortality rate.…”

Section: Introductionmentioning

confidence: 99%

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

View full text Add to dashboard Cite

Background Rheumatoid arthritis (RA) is associated with joint damage. Effectiveness of embelin has been established in a wide variety of inflammatory disorders, but its utility as a therapeutic agent is limited by its poor absorption, rapid metabolism, and fast systemic elimination. To apprehend these limitations, we propose to use highly bioavailable embelin‐loaded chitosan nanoparticles (CS‐embelin NPs) for the treatment of RA. Methods The rats were made arthritic using a subcutaneous injection with 0.1 ml complete Freund's adjuvant (CFA) into the footpad of the left hind paw. CS‐embelin NPs (25 and 50 mg/kg) was administered from day 15 to day 28 after adjuvant injection. After the experimental period, the animals were sacrificed and various biochemical markers were assessed. Results Arthritic score and paw swelling were significantly reduced after treatment with CS‐embelin NPs. Arthritis‐induced rats showed a significant increase in malondialdehyde (MDA) and nitric oxide (NO) with a concomitant reduction of antioxidants in the paw tissue. CS‐embelin NPs (25 and 50 mg/kg) reduced MDA and NO levels and restored antioxidant levels to normalcy by mitigating oxidative stress. The arthritic rats exhibited elevated tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and interleukin‐1beta (IL‐1β) serum concentrations, upregulated TNF‐ α and IL–6 protein levels and upregulated nuclear factor‐kB (NF‐kB) mRNA expression in paw tissues. Treatment with CS‐embelin NPs (25 and 50 mg/kg) significantly reduced serum levels and down‐regulated inflammatory markers to normalcy, dose‐dependently. Conclusion The results suggest that CS‐embelin NPs displayed a protective effect against adjuvant‐induced arthritis in rats mediated through antioxidant and anti‐inflammatory effects.

show abstract

Section: Introductionmentioning

confidence: 99%

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

View full text Add to dashboard Cite

show abstract

“…Furthermore, for the absorbance value at 320 nm, the number of interproducts is linear with time. The antioxidant can react with the superoxide anion and effectively prevent the accumulation of intermediates, thus reducing the absorbance of the solution at 320 nm . Thus, hydrogel’s superoxide anion scavenging ability can be determined by measuring the absorbance value of the system at 320 nm.…”

Section: Resultsmentioning

confidence: 99%

“…The antioxidant can react with the superoxide anion and effectively prevent the accumulation of intermediates, thus reducing the absorbance of the solution at 320 nm. 59 Thus, hydrogel's superoxide anion scavenging ability can be determined by measuring the absorbance value of the system at 320 nm. As shown in Figure 2c,f, the results show that the scavenging effect of EA is higher than that of PC, indicating that EA plays a major role in scavenging superoxide anion free radicals.…”

Section: Superoxide Anion Radical Scavenging O-triphenylmentioning

confidence: 99%

Protective Effect of Composite Hydrogel Based on Hydroxypropyl Trimethylammonium Chloride Chitosan on Skin Photodamage

Jin

Zhao

2022

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Long-term or excessive exposure to UV light can lead to sunburn, erythema, melanin deposition, inflammation, and even skin cancer. This study used composite hydrogels with N-2-hydroxypropyl trimethylammonium chloride chitosan (N-2-HACC) and hyaluronic acid (HA) as carriers. In addition, ellagic acid (EA) and proanthocyanidins (PC) as functionalized materials were prepared. The infrared results show that the N-2-HACC/HA/EA/PC hydrogel is formed by N-2-HACC connecting with HA by electrostatic interaction, and HA forms hydrogen bonds between EA and PC. The in vitro antioxidant results show that EA and PC have different clearance efficiency of DPPH and superoxide anion radicals at different pH values. Thus, they have complementary effects on antioxidant properties. The cumulative release results demonstrate that the hydrogels had an excellent slow-release rate at different pHs. The animal experiments reveal that N-2-HACC/HA/EA/PC could restore the consumption of antioxidant enzymes induced by UVB irradiation and inhibit matrix metalloproteinase 1. N-2-HACC/HA/EA/PC can inhibit UVB-induced histological damage, such as epidermal thickening, collagen fiber, and elastic fiber destruction. N-2-HACC/HA/EA/PC has a significant photoprotective effect by protecting the activities of superoxide dismutase, catalase, and glutathione peroxidase and inhibiting the activity of inflammatory factors. N-2-HACC/HA/EA/PC composite hydrogel system shows comprehensive performance. Its ability to scavenge free radicals may act as an antioxidant to protect mice from skin cancer.

show abstract

“…The overall clearance effects in this indicator were slightly lower than the free radical scavenging ability of DPPH and ABTS. It can be surmised that the superoxide radical was mainly scavenged by intracellular superoxide dismutase, while the scavenging capacity was limited in vitro chemical methods ( Azmi et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Composition analysis and antioxidant activity evaluation of a high purity oligomeric procyanidin prepared from sea buckthorn by a green method

Zhu

Yuen²,

Li³

et al. 2021

Current Research in Food Science

View full text Add to dashboard Cite

Procyanidin is an important polyphenol for its health-promoting properties, however, the study of procyanidin in sea buckthorn was limited. In this paper, sea buckthorn procyanidin (SBP) was obtained through a green isolation and enrichment technique with an extraction rate and purity of 9.1% and 91.5%. The structure of SBP was analyzed using Ultraviolet–visible spectroscopy (UV–vis), Fourier-transform infrared spectroscopy (FT-IR), and liquid chromatography-mass spectrometry (LC-MS/MS). The results show that SBP is an oligomeric procyanidin, mainly composed of (−)-epicatechin gallate, procyanidin B, (+)-gallocatechin-(+)-catechin, and (+)-gallocatechin dimer. SBP showed superior scavenging capacity on free radicals. Furthermore, the cleaning rate of the ABTS radical was 4.8 times higher than vitamin C at the same concentration. Moreover, SBP combined with vitamin C presented potent synergistic antioxidants with combined index values below 0.3 with concentration rates from 5:5 to 2:8. SBP also provided significant protection against oxidative stress caused by hydrogen peroxide (H 2 O 2 ) on RAW264.7 cells. These findings prove the potential of SBP as a natural antioxidant in food additives and support the in-depth development of sea buckthorn resources.

show abstract

Oxidative free radicals scavenging activity (in vitro and in vivo assay) of standardized fractions from the seeds of Argyreia speciosa (Ghav-patta) a traditional Indian medicine

Abstract: Graphical abstract

Cited by 10 publications

References 27 publications

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Protective Effect of Composite Hydrogel Based on Hydroxypropyl Trimethylammonium Chloride Chitosan on Skin Photodamage

Composition analysis and antioxidant activity evaluation of a high purity oligomeric procyanidin prepared from sea buckthorn by a green method

Contact Info

Product

Resources

About