Oxidative insult to human red blood cells induced by free radical initiator AAPH and its inhibition by a commercial antioxidant mixture

Zou, Cheng‐Gang; Agar, Nicholas; Jones, Graham L.

doi:10.1016/s0024-3205(01)01112-2

Cited by 114 publications

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“…It is strongly suggested that the effects of oxidative stress on membrane fluidity of RBCs might be mediated, at least in part, by reducing the NO-bioavailability, although direct actions of oxidative stress on membrane structural and functional properties cannot be excluded. 19,20) Further studies should be performed to assess more precisely the role of oxidative stress in the regulation of membrane functions in hypertension.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Membrane Fluidity of Red Blood Cells in Hypertensive and Normotensive Men An Electron Spin Resonance Investigation

Tsuda

2010

Int. Heart J.

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SummaryRecent evidence indicates that oxidative stress might actively participate in the pathophysiology of hypertension, atherosclerosis, and other cardiovascular diseases. The purpose of the present study was to assess the possible link between oxidative stress and membrane fluidity in hypertensive and normotensive men. We measured the membrane fluidity (a reciprocal value of membrane microviscosity) of red blood cells (RBCs) in hypertensive and normotensive men using an electron spin resonance (ESR) and spin-labeling method. Membrane fluidity of RBCs was decreased in hypertensive men compared with normotensive men. The levels of plasma 8-Iso-prostaglandin F2α (8-Iso-PG F2α : an index of oxidative stress) were significantly higher in hypertensive men than in normotensive men. In contrast, plasma nitric oxide (NO)-metabolite levels were significantly lower in hypertensive men than in normotensive men. In the overall analysis of hypertensive and normotensive men, plasma 8-Iso-PG F2α levels were inversely correlated with plasma NO-metabolites. Furthermore, the reduced membrane fluidity of RBCs was associated with increased plasma 8-Iso-PG F2α and decreased plasma NO-metabolite levels. In a multivariate regression analysis, plasma 8-Iso-PG F2α was found to be an independent determinant of membrane fluidity of RBCs participate in the pathophysiology of hypertension and atherosclerosis, and might be associated with increased risk of cardiovascular diseases, vascular dysfunction, and the metabolic syndrome.1-3) Recent studies have shown that plasma 8-Iso-prostaglandin F2α (8-Iso-PG F2α ) may be a reliable index of oxidative stress in humans. [4][5][6][7] It was demonstrated that the plasma concentration of 8-Iso-PG F2α was significantly increased in subjects with essential hypertension compared with normotensive subjects. 4,5) It has also been shown that plasma 8-Iso-PG F2α levels were elevated in patients with coronary artery disease, particularly in those with hypertension. 6,7) However, cellular mechanisms of oxidative stress in the pathophysiology of hypertension are not fully understood.Abnormalities in the physical properties of the cell membranes may underlie the defects that are strongly linked to hypertension, stroke, and other cardiovascular disease conditions. 8,9) An electron spin resonance (ESR) and spinlabeling method has been developed to evaluate the membrane fluidity (a reciprocal value of membrane microviscosity) and the perturbations of membrane function by external agents. 8,9) Using the ESR method, we have been performing a series of experiments regarding the membrane fluidity in subjects with essential hypertension. [9][10][11][12][13] The results suggest that the membrane fluidity of red blood cells (RBCs) was significantly lower in hypertensive subjects than in normotensive subjects, indicating that the cell membranes were stiffer and less fluid in essential hypertension. [9][10][11][12][13] Because the deformability of RBCs may be highly dependent on the membrane fluidity, 8) the reduction in...

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Membrane Fluidity of Red Blood Cells in Hypertensive and Normotensive Men An Electron Spin Resonance Investigation

Tsuda

2010

Int. Heart J.

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“…The highest protective effect was found in Jihlsyrups, which possessed the highest half hemolysis value (317.79 min) whereas, the lowest half hemolysis value (226.44 min) was found using Tamaajountsyrups. The important half hemolysis value (52.17min) illustrated in the negative control may be due to the endogenous antioxidants in the erythrocytes which can trap radicals to protect them against free radical induced hemolysis as described previously ( Zou et al, 2001). The high half hemolysis value observed for all date fruit syrups compared to negative control show that these extracts did not provide just the protective effect but also the stabilizing effect of erythrocytes membrane.…”

Section: Evaluation Antioxidant Activitiesmentioning

confidence: 59%

“…The Anti-hemolytic effect of date fruit syrups: Erythrocytes membrane lipids are rich in polyunsaturated fatty acids and therefore the exposure to free radical generated from the degradation of AAPH at physical temperature is the reason for hemolysis (Zou et al, 2001). Table 4 shows the protective effects of date fruit syrups and Trolox on the hemolysis induced by AAPH.…”

Section: Evaluation Antioxidant Activitiesmentioning

confidence: 99%

Antioxidant and Anti-Hemolytic Activities of Phenolic Constituents of Six Moroccan Date Fruit (Phoenix Dactylifera L.) Syrups

Bouhlali¹,

Alem²,

Benlyas³

et al. 2015

JGIASS

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Date fruits are traditionally used to prepare a wide range of products such as vinegar wine, jam and syrup named locally "Tahlawt" moreover, their direct consumption. The aim of this study is to investigate the antioxidant and anti-hemolytic activities of six syrups prepared traditionally form six different date fruit cultivars grown in southeast Morocco. Significant difference(P < 0.05) was established among analysed syrups. The highest phenolic (6.70 g GAE /100g DW) and flavonoid content (932.82 mg RE /100g DW) were found in Jihl syrups which possessed the highest antioxidant activity based on FRAP (9.55 mmol TE/100g DW), ABTS (8.27 mmol TE /100 g DW), DPPH IC50 (381.99 µg/mL) and exhibited the highest membrane protective effect (317.70 min). Tamaajount syrup contains the lowest phenolic (3.72g GAE /100g DW) and flavonoid content (528.19 mg RE /100g DW) and presented the lowest antioxidant activity based on FRAP (5.30 mmol TE/100g DW), ABTS (4.68 mmol TE /100 g DW) and DPPH IC50 (1.095 mg/mL) as well as the lowest membrane protective effect (226.44 min).The results obtained suggest that date fruit syrups could be considered as a functional food or functional food ingredient because of their high phenolic compounds, which act as antioxidants and membrane stabilizer.

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“…According to Zou et al (2001), the depletion of GSH and, subsequently, lipid peroxidation, begins the process of oxidative damage to erythrocytes mediated by ROO , and when the concentration of TBARS reaches a critical level and the degradation of membrane proteins occurs, significant hemolysis can be observed. Hseu et al (2002) GSH has a fundamental role to protect erythrocytes against oxidant molecules.…”

Section: Resultsmentioning

confidence: 99%

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

Ribeiro

Berto

Ribeiro

et al. 2015

Pharmaceutical Biology

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(2015) Stem bark and flower extracts of Vismiacauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes, Pharmaceutical Biology, 53:11, 1691-1698, DOI: 10.3109/13880209.2014 Previous research on V. cauliflora extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species.Objective: To determine the activity of stem bark and flower extracts of V. cauliflora on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes. Materials and methods:The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3-1000 mg/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress. Results: Vismia cauliflora extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC 50 515 mg/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the tert-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7-18 mg/mL). Furthermore, only stem bark extract (100 mg/mL) was able to inhibit the depletion of glutathione (13%). Discussion and conclusion: These results reinforce the therapeutic potential of stem bark and flower extracts of V. cauliflora to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.

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Oxidative insult to human red blood cells induced by free radical initiator AAPH and its inhibition by a commercial antioxidant mixture

Cited by 114 publications

References 0 publications

Oxidative Stress and Membrane Fluidity of Red Blood Cells in Hypertensive and Normotensive Men An Electron Spin Resonance Investigation

Oxidative Stress and Membrane Fluidity of Red Blood Cells in Hypertensive and Normotensive Men An Electron Spin Resonance Investigation

Antioxidant and Anti-Hemolytic Activities of Phenolic Constituents of Six Moroccan Date Fruit (Phoenix Dactylifera L.) Syrups

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

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