Oxidative Stress and Antioxidant Systems in Crustacean Life Cycles

Fanjul‐Moles, María Luisa; Gonsebatt, María E.

doi:10.1002/9781444345988.ch15

Cited by 16 publications

(11 citation statements)

References 109 publications

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“…Similarly, dietary B. subtilis enhanced the antioxidant defence by increasing SOD activity in L. vannamei juveniles (Shen et al, 2010). Endogenous reactive oxygen species (ROS) is produced continuously in crustaceans through their metabolic process (Fanjul-Moles & Gonsebatt, 2011), and excessive ROS can induce tissue damage (Halliwell & Gutteridge, 2015). The SOD antioxidant enzyme could react instantly and correspondingly to the production of ROS when prawns are under oxidative stress, which is believed to contribute to the correlation between the immune response and antioxidant enzyme activity (Gopalakrishnan et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

et al. 2018

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This study was conducted to investigate the effects of dietary probiotic quorum sensing degrader Bacillus cereus on the growth performance, intestinal short‐chain fatty acid levels, antioxidant status and histopathological response of Macrobrachium rosenbergii against Aeromonas hydrophila. M. rosenbergii juveniles were fed with 104 cfu/g probiotic B. cereus, while the control group was fed without B. cereus. After 28 days of feeding, growth performance was significantly higher (p < 0.05) in prawns fed with B. cereus compared to the control group. Probiotic‐fed prawns showed the highest propionic acid level, while no significant differences (p > 0.05) were observed for acetic acid or butyric acid levels. Dietary B. cereus supplementation significantly (p < 0.05) improved the superoxide dismutase (SOD) activity in the prawns. In the challenge study, no significant differences were observed between the treatments in survival and haemolymph SOD activity. However, histopathological observations of the hepatopancreas after A. hydrophila challenge showed hemocyte infiltrations and necrosis in the control prawns, while the probiotic‐fed prawns showed an overall better hepatopancreatic condition. These findings indicate that dietary B. cereus can be an effective growth promoter in M. rosenbergii and mitigates a compromise to the hepatopancreatic integrity during pathogenic challenge.

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Section: Discussionmentioning

confidence: 99%

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

et al. 2018

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“…It is well established that levels of ROS may become elevated in the presence of heavy metals and other environmental pollutants, and that excessive amounts of ROS can damage macromolecules including lipids and proteins (Lesser 2006 ; Lushchak et al 2011 ). SOD is known to be an important part of the enzymatic antioxidant systems in aquatic arthropods (Fanjul-Moles and Gonsebatt 2011 ). However, investigations of either the Cu/Zn (cytosolic) or Mn (mitochondrial) forms of this enzyme in horseshoe crabs (which are chelicerates, not crustaceans) are lacking, aside from a report by Ding et al ( 2005 ) that showed upregulation of Cu SOD, along with other stress-related enzymes including Hsp’s 90, 70, and 40 as part of the response to excessive ROS when mangrove horseshoe crabs ( Carcinoscorpius rotundicauda ) were infected with Gram-negative bacteria ( Pseudomonas aeruginosa ).…”

Section: Discussionmentioning

confidence: 99%

“…The lack of a significant increase in either SOD levels or specific activity differs from a number of other studies with other aquatic animals. Increases in SOD and other antioxidant molecules are commonly reported from crustaceans and other aquatic invertebrates exposed to pollution stress (Fanjul-Moles and Gonsebatt 2011 ). For example, Lyu et al ( 2013 ) found significant increases in Cu/Zn SOD transcription and in SOD enzymatic activity in the cladoceran Daphnia magna after 48 h exposure to 5 μg/L CuCl 2 .…”

Section: Discussionmentioning

confidence: 99%

“…In marine crustaceans, cellular damage from heavy metals may also be modulated through binding by metallothioneins, sequestration in metal-rich vacuoles, and other mechanisms (Ahearn et al 2004 ), but these have not yet been studied in horseshoe crabs. Future research should investigate other potential mechanisms to protect against damage by ROS, including other enzymes such as catalase and peroxidases, and cytosolic scavengers molecules such as ascorbic acid, tocopherol, glutathione, and carotenoids (Lesser 2006 ; Fanjul-Moles and Gonsebatt 2011 ; Regoli and Giuliani 2014 ).…”

Section: Discussionmentioning

confidence: 99%

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Effects of copper and cadmium on development and superoxide dismutase levels in horseshoe crab (Limulus polyphemus) embryos

et al. 2015

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Pollution by metals may adversely affect organisms through the generation of reactive oxygen species (ROS). In this study, we examined the sublethal effects of two metals, copper and cadmium, on horseshoe crab (Limulus polyphemus) embryos. Exposure to copper or cadmium at concentrations of 0.01–10 mg/L for periods of 4, 8, 16 and 24 h had minimal effect on embryo survival except at 100 mg/L Cu. However, metal-exposed embryos took significantly longer to hatch into first instar (“trilobite”) larvae than seawater controls. Levels of superoxide dismutase (SOD), believed to be important in the response to oxidative stress, were determined by Western blotting. Both the Cu/Zn and Mn cofactor forms of SOD tended to be somewhat elevated in metal-exposed embryos, but the increases were neither dose nor time-dependent. Likewise, SOD enzymatic activity showed no significant differences comparing embryos exposed to metals with seawater controls. We conclude that the protective role of SOD’s against ROS produced in response to metal exposure appears to be limited in horseshoe crab embryos, at least under our experimental conditions.

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“…En L. albus, la ingesta de alimento podría variar durante el año incrementando el contenido de ascorbato para prevenir el daño oxidativo en el medio hidrofílico del tejido (Malanga et al, 2009). Particularmente en la centolla del Canal Beagle, el consumo de macroalgas es mayor durante el verano y el otoño (60-80% de frecuencia de ocurrencia; Comoglio, 1994 La hiperglucemia ha sido observada en respuesta a factores de estrés como: la hipoxia en los cangrejos Parastacus defossus y P. brasiliensis (da Silva-Castiglioni et al, 2010), la anaerobiosis ambiental en el cangrejo Neohelice granulata (Oliveira et al, 2001), y la muda en el cangrejo Procambarus clarkii (Fanjul-Moles & Gonsebatt, 2011). Además, las dietas ricas en carbohidratos causan altas concentraciones de glucosa en los diferentes tejidos, especialmente en la hemolinfa de los cangrejos Aegla ligulata y Ocyopode quadrata (Oliveira et al, 2004;Vinagre et al, 2007).…”

Section: Antioxidantes No -Enzimáticosunclassified

Baseline defense system of commercial male king crab Lithodes santolla from the Beagle Channel

Schvezov

Lovrich

Florentín

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Environmental and physiological variations influence the steady-state concentration of free oxygen radicals in cells. Because of the seasonal life cycle of Lithodes santolla in the Beagle Channel, a baseline study of the antioxidant physiological variations along the seasons is necessary for a better understanding of its ecophysiology. The aim of this study was to evaluate the seasonal variations in gills, hemolymph, muscle and hepatopancreas of the: i) enzymatic activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione transferase; ii) ascorbic acid and total glutathione; iii) lipid peroxidation and protein oxidation; iv) glucose, proteins and pH. Seasonality found in the antioxidant defense system of L. santolla from the Beagle Channel acts in a collaborative way during the most relevant life cycle phases (reproduction and molting), avoiding a long term oxidative stress. The antioxidant system also shows changes in the enzymatic activities likely caused by the environmental factors, such as low temperatures during winter and spring seasons.

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Oxidative Stress and Antioxidant Systems in Crustacean Life Cycles

Cited by 16 publications

References 109 publications

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

Effects of copper and cadmium on development and superoxide dismutase levels in horseshoe crab (Limulus polyphemus) embryos

Baseline defense system of commercial male king crab Lithodes santolla from the Beagle Channel

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