Oxidative stress in sickle cell disease: An overview of erythrocyte redox metabolism and current antioxidant therapeutic strategies

Silva, Danilo Grünig Humberto; Junior, Edis Belini; Almeida, Eduardo Alves de; Bonini-Domingos, Cláudia Regina

doi:10.1016/j.freeradbiomed.2013.08.181

Cited by 100 publications

(112 citation statements)

References 150 publications

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“…Les résultats obtenus pourraient s'expliquer en partie par le fait que l'hémoglobine S constitue un facteur de risque de peroxydation pour la membrane érythrocytaire (Silva et al, 2013). Ainsi, chez ces patients, la peroxydation lipidique au niveau des membranes des globules rouges jouerait un rôle dans l'aggravation de l'anémie.…”

Section: Discussionunclassified

“…Dans une étude antérieure (Gueye et al, 2006), nous avons montré que l'hémoglobine extra-érythrocytaire pouvait induire, sur le globule, un stress oxydant se traduisant par une lyse précoce. En effet, en dehors du globule rouge et de son contenu antioxydant, la toxicité oxydative de l'Hb s'affirme et va produire, via la réaction de Fenton, des radicaux libres qui vont attaquer la membrane des globules rouges et entraîner leur lyse précoce (Silva et al, 2013). L'action anti-oxydante de l'haptoglobine étant phénotype-dépendante (Asleh et al, 2005 ;Gueye et al, 2006), cela pourrait expliquer en partie le fait que l'anémie retrouvée chez les patients de type SS soit plus intense chez les patients de type Hp2-2 comparée à celle retrouvée au niveau des 2 autres phénotypes.…”

Section: Discussionunclassified

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Aggravation de l’anémie et polymorphisme de l’haptoglobine au cours de la drépanocytose au Sénégal

Gueye

Fatou²,

Seck³

et al. 2014

Int. J. Bio. Chem. Sci

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Section: Discussionunclassified

Aggravation de l’anémie et polymorphisme de l’haptoglobine au cours de la drépanocytose au Sénégal

Gueye

Fatou²,

Seck³

et al. 2014

Int. J. Bio. Chem. Sci

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“…Thiol oxidation has also been associated with both inhibition of the flippase and stimulation of the scramblase (Morrot et al , 1989; Connor & Schroit, 1990; Devaux & Zachowski, 1994; Martin & Jesty, 1995; de Jong & Kuypers, 2006). In addition, the normal antioxidant capacity of the red cell is compromised in SCA patients, with low availability of antioxidant enzymes, reduced levels of glutathione (GSH) and also of non‐enzymatic antioxidants, such as vitamins C and E (Silva et al , 2013). Oxidative damage has also been associated with deleterious effects on red cell cation balance.…”

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confidence: 99%

Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

et al. 2018

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SummaryPhosphatidylserine (PS) exposure increases as red cells age, and is an important signal for the removal of senescent cells from the circulation. PS exposure is elevated in red cells from sickle cell anaemia (SCA) patients and is thought to enhance haemolysis and vaso‐occlusion. Although precise conditions leading to its externalisation are unclear, high intracellular Ca2+ has been implicated. Red cells from SCA patients are also exposed to an increased oxidative challenge, and we postulated that this stimulates PS exposure, through increased Ca2+ levels. We tested four different ways of generating oxidative stress: hypoxanthine and xanthine oxidase, phenazine methosulphate, nitrite and tert‐butyl hydroperoxide, together with thiol modification with N‐ethylmaleimide (NEM), dithiothreitol and hypochlorous acid (HOCl), in red cells permeabilised to Ca2+ using bromo‐A23187. Unexpectedly, our findings showed that the four oxidants significantly reduced Ca2+‐induced PS exposure (by 40–60%) with no appreciable effect on Ca2+ affinity. By contrast, NEM markedly increased PS exposure (by about 400%) and slightly but significantly increased the affinity for Ca2+. Dithiothreitol modestly reduced PS exposure (by 25%) and HOCl had no effect. These findings emphasise the importance of thiol modification for PS exposure in sickle cells but suggest that increased oxidant stress alone is not important.

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“…However, oxidative stress occurs when the ROS overwhelm the cellular antioxidant defence system, either by increased ROS generation or by decreased cellular antioxidant capacity. The threat of oxidative stress is so real as it results in macromolecular damage implicated in many diseases including but not limited to sickle-cell disease [8], atherosclerosis, diabetes, carcinogenesis, and inflammatory diseases, just to mention but few [7].…”

Section: Introductionmentioning

confidence: 99%

“…Based on our recent ethnopharmacological surveys of medicinal plants from the state of Mato Grosso, Brazil [1][2][3][4][5][6][7][8][9][10][11][12][13] and literature review [14], we carried out screening of selected plants for their antibacterial, antioxidative and antiradical activities. We also evaluated the linear correlation between the selected secondary metabolites classes present in the extracts to their in vitro antioxidant and anti-radical activities.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Selected Plants Used by Populations From Juruena Valley, Legal Amazon, Brazil

Silva

Arunachalam

Miyajima

et al. 2017

Int J Pharm Pharm Sci

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Objective: The purpose of this study was to evaluate selected Brazilian plants from Juruena valley region of Mato Grosso, for their in vitro antimicrobial and antioxidant activities. Methods:The powder obtained from different parts of the twenty-six (26) plants were macerated in hydroethanolic solution to obtain the extracts. The hydroethanolic extracts were tested for their in vitro antimicrobial activity by determining the MIC using broth microdilution. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) methods were used for the determination of antioxidant activities. Correlation between classes of secondary metabolites and antioxidant activity was assessed.Results: Phanera glabra extract (HEPg) showed broad antibacterial spectrum, presenting the best activity against Klebsiella pneumoniae. Hevea microphylla extract (HEHm) presented a narrow spectrum of antibacterial activity with strong effect against Shigella flexneri. The only plant with broad spectrum antifungal activity was Bertholletia excelsa (HEBe), with moderate activity against strains of Aspergillus and Candida. The following extracts were prominent regarding their activities in the DPPH and FRAP assays-HEBe, Cariniana rubra (HECr) and in the FRAP assay alone, Cedrela odorata (HECo) and HEPg. None of the extracts was active in the NO assay. A significant association was observed between DPPH activity and the total phenolic contents. Conclusion:Our results justified the use of some of the investigated plants in the Brazilian ethnomedicine. The antibacterial activities of these plants are bacteriostatic in nature. These findings support that a number of investigated plants could be a valuable source of new antioxidant and antimicrobial compounds that can potentially deliver novel mechanisms of actions. However, further studies are required.

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Oxidative stress in sickle cell disease: An overview of erythrocyte redox metabolism and current antioxidant therapeutic strategies

Cited by 100 publications

References 150 publications

Aggravation de l’anémie et polymorphisme de l’haptoglobine au cours de la drépanocytose au Sénégal

Aggravation de l’anémie et polymorphisme de l’haptoglobine au cours de la drépanocytose au Sénégal

Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

Antimicrobial and Antioxidant Activities of Selected Plants Used by Populations From Juruena Valley, Legal Amazon, Brazil

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