Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Abstract

Oxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 ± 0.76 nmol/mg protein), AOPP (14.99 ± 4.67 nmol/mg protein) levels, and SOD activities (7.49 ± 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 ± 0.14 nmol/mg protein, AOPP 13.12 ± 3.86 nmol/mg protein, SOD 4.82 ± 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.

Functionally modified, melt‐electrospun thermoplastic polyurethane mats for wound‐dressing applications

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Plasma polymerization‐modified bacterial polyhydroxybutyrate nanofibrillar scaffolds

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Changes of Antioxidant Function and the mRNA Expression Levels of Xanthine Oxidase in Primary Chick Kidney Cell Culture Caused by Nephropathogenic Infectious Bronchitis Virus Infection

“…The equilibrium between the ambient levels of the superoxide anion and cellular antioxidant capacity provided by SOD activity was broken at 72hpi. SOD are the free radical scavenger which can remove body's free radical, it have anti-oxidative stress function to maintaining body's in a healthy balance between oxidants and antioxidants (Han et al, 2014) MDA is a biomarker of lipid per-oxidation and its content represent the level of body's oxidative stress (Demirbilek et al, 2011). Therefore, determination of MDA and SOD can reflect the capability of elimination of free radicals in the body and the extent of CK cells injury during IBV infection (Zhou et al, 2006).…”