Oxygen consumption during and post-hypoxia exposure in bearded fireworms (Annelida: Amphinomidae)

Grimes, Candace J.; Capps, Crystal; Petersen, Lene H.; Schulze, Anja

doi:10.1007/s00360-020-01308-y

Cited by 7 publications

(10 citation statements)

References 64 publications

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“…Also, the down-regulation of certain metabolic genes associated with aerobic metabolism when comparing the animals exposed to lowered DO conditions to those of their natural habitat indicates the overall metabolic depression of worms exposed to chronic hypoxia. These results therefore support a previous study demonstrating lowered oxygen uptake in chronic hypoxic fireworms 27 .…”

Section: Discussionsupporting

confidence: 93%

“…Many of the gene expression responses that we observed are consistent with previous observations. For example, the higher expression levels of genes related to epithelial morphogenesis are consistent with the increase in surface branchial surface area 39 ; likewise, expression patterns of metabolism-related genes correspond with our observation of oxygen uptake 27 . Other observed trends, such as the expression patterns of genes in the HIF or Notch pathways, can guide future biochemical and physiological studies in H. carunculata or other species.…”

Section: Discussionsupporting

confidence: 86%

“…For the purpose of this study, we characterize DO concentrations of 4.5 mg O 2 L −1 as Mild hypoxia 25 , 26 , 2.5 mg O 2 L −1 as Moderate hypoxia, and 1 mg O 2 L −1 as Severe hypoxia. Our previous studies indicate that previous exposure to the Moderate hypoxia increases H. carunculata ’s metabolic rate in the long-term 27 .…”

Section: Introductionmentioning

confidence: 91%

“…Previous work has focused on morphological changes and oxygen uptake in H. carunculata in response to hypoxia 27 , 38 , 39 . Most notably, branchial surface area increases in response to both intermittent 38 and chronic 39 hypoxia.…”

Section: Introductionmentioning

confidence: 99%

“…Most notably, branchial surface area increases in response to both intermittent 38 and chronic 39 hypoxia. While the effect on oxygen uptake rates during hypoxia is small 27 , 38 , hypoxia exposure results in a long-term elevation of the metabolic rate, potentially leading to increased feeding activity 27 . Differential gene expression will help detect additional physiological processes that affect overall organismal functioning and find explanations for the environmental hardiness of H. carunculata .…”

Section: Introductionmentioning

confidence: 99%

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Differential gene expression indicates modulated responses to chronic and intermittent hypoxia in corallivorous fireworms (Hermodice carunculata)

Grimes

Petersen

Schulze

2021

Sci Rep

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Climate models predict an increase in extent, frequency, and duration of marine hypoxia events in the twenty first century. A better understanding of organismal responses to hypoxia in individual species is a crucial step for predicting ecosystem responses. We experimentally subjected a common invertebrate, the bearded fireworm (Hermodice carunculata) to two levels of chronic hypoxia and, in a separate experiment, to intermittent hypoxia. We found components of the conserved hypoxia-inducible factor (HIF) pathway and show a modulated response to hypoxia depending on the severity of hypoxic stress: under mild hypoxia, only the HIF-1α subunit is upregulated, while expression of the other subunit, aryl hydrocarbon nuclear translator, only increases significantly at more severe hypoxia levels. The chronic trials revealed down-regulation of genes related to cell adhesion, transport, development and heme-binding, and up-regulation of genes related to glycolysis, oxygen binding, cell differentiation, digestive and reproductive function. The intermittent hypoxia trials revealed an upregulation of heme transporter activity during hypoxia, and our time series analysis characterized nine clusters of genes with similar expression patterns. Our findings suggest that H. carunculata is likely to tolerate, and be resilient to, predicted future hypoxia conditions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differential gene expression indicates modulated responses to chronic and intermittent hypoxia in corallivorous fireworms (Hermodice carunculata)

Grimes

Petersen

Schulze

2021

Sci Rep

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Effects of Asynchronous Stressors on the Eastern Oyster (Crassostrea virginica)

Agrawal

Jurgens

2023

Estuaries and Coasts

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With intensifying global change ecologists work to understand the effects of anthropogenic and environmental stressors on organisms. Importantly, prior stress can influence population response to later stress. This is often seen in population response to acute stress during extreme events, which may occur after exposure to other chronic environmental stressors. However, experiments on the effects of asynchrony in which stresses are imposed at different times on organisms are rare. I reviewed this small but growing body of literature and show how results in studies incorporating asynchrony often differ from those that examine the effects of multiple stressors imposed on organisms at the same time. In particular, I found that asynchrony rarely leads to additive effects, but rather elicited responses of cross-tolerance (mitigation of latter stress) or cross-susceptibility (exacerbation of the latter stress). Additionally, the majority of asynchronous stressor studies were conducted in North America and Europe and were rare in the rest of the world. I also conducted an experiment on Eastern oysters (Crassostrea virginica), for which there are few asynchronous stressor studies. In Galveston Bay, Texas, the hurricane season begins in May, following mild spring water temperatures, but continues through late summer months, when water temperatures can reach 32°C. I asked if oysters respond differently to hurricane-level low-salinity stress after periods of elevated late summer temperatures relative to more standard, earlyseason conditions. I exposed newly settled oyster spat to early-season (24°C) or lateseason high temperatures (32°C) for one month followed by an acute low salinity (1 ppt) disturbance for 10 days (versus control). Oysters experienced the highest mortality iii (46%) when high-temperature and low salinity stresses were imposed simultaneously with no prior conditioning. This was significantly different from oysters under asynchronous stressor treatments, which saw only 4% mortality. Additionally, prior thermal stress mitigated some of the negative effects of subsequent osmotic stress leading to significantly lower mortalities (4%) compared to treatments under acute salinity stress without prior heat exposure (16%). Results suggest that incorporating temporal dynamics, rather than simply crossing multiple stressors simultaneously, can have important consequences for our understanding of impacts from extreme events on early, important life stages of organisms.Masami Fujiwara, for their support through the course of my master's thesis. Additionally, I could not have seen my experiment through to completion without the help of Katie St. Clair and the Sea Life Facility. Thank you also to Dr. Scott Rikard at the Auburn Island Shellfish Laboratory for shipping me oyster spat and providing me with husbandry knowledge. A special thank you to my lab members Emily Hubbard and Melissa Ciesielski. Their support during my experiment was unparalleled. Additional thanks to Ume, Olivia, Candace, Janelle, Rayna, and Stephani...

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Hypoxia in aquatic invertebrates: Occurrence and phenotypic and molecular responses

Lee,

Byeon,

Kim

et al. 2023

Aquatic Toxicology

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Oxygen consumption during and post-hypoxia exposure in bearded fireworms (Annelida: Amphinomidae)

Cited by 7 publications

References 64 publications

Differential gene expression indicates modulated responses to chronic and intermittent hypoxia in corallivorous fireworms (Hermodice carunculata)

Differential gene expression indicates modulated responses to chronic and intermittent hypoxia in corallivorous fireworms (Hermodice carunculata)

Effects of Asynchronous Stressors on the Eastern Oyster (Crassostrea virginica)

Hypoxia in aquatic invertebrates: Occurrence and phenotypic and molecular responses

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