Oxygen Distributions in Tissue Measured by Phosphorescence Quenching

Wilson, David F.; Vinogradov, Sergei A.; Grosul, Pavel; Rozhkov, Vladimir V.; Dunphy, Isolde; Shuman, Lee; Dugan, Benjamin W.; Evans, Sydney M.

doi:10.1007/978-1-4615-0205-0_30

Cited by 22 publications

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References 8 publications

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“…A calibration curve based on Stern–Volmer kinetics 27 , 28 was used to convert the measured lifetime (τ) to the absolute pO2. The calibration of Oxyphor PtG4 under physiological conditions is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke

et al. 2018

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, "Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke," Neurophoton. 5(3), 035003 (2018), doi: 10.1117/1.NPh.5.3.035003. Abstract. We present a dual-modality imaging system combining laser speckle contrast imaging and oxygendependent quenching of phosphorescence to simultaneously map cortical blood flow and oxygen tension (pO 2 ) in mice. Phosphorescence signal localization is achieved through the use of a digital micromirror device (DMD) that allows for selective excitation of arbitrary regions of interest. By targeting both excitation maxima of the oxygen-sensitive Oxyphor PtG4, we are able to examine the effects of excitation wavelength on the measured phosphorescence lifetime. We demonstrate the ability to measure the differences in pO 2 between arteries and veins and large changes during a hyperoxic challenge. We dynamically monitor blood flow and pO 2 during DMDtargeted photothrombotic occlusion of an arteriole and highlight the presence of an ischemia-induced depolarization. Chronic tracking of the ischemic lesion over eight days revealed a rapid recovery, with the targeted vessel fully reperfusing and pO 2 returning to baseline values within five days. This system has broad applications for studying the acute and chronic pathophysiology of ischemic stroke and other vascular diseases of the brain. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Methodsmentioning

confidence: 99%

Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke

et al. 2018

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Imaging the Hypoxic Tumor Microenvironment in Preclinical Models

2013

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Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia

Wisniewski

Nichols

Gamsey

et al. 2017

Advances in Experimental Medicine and Biology

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We describe a simple method of tracking oxygen in real-time with injectable, tissue-integrating microsensors. The sensors are small (500 μm × 500 μm × 5 mm), soft, flexible, tissue-like, biocompatible hydrogels that have been shown to overcome the foreign body response for longterm sensing. The sensors are engineered to change luminescence in the presence of oxygen or other analytes and function for months to years in the body. A single injection followed by noninvasive monitoring with a hand-held or wearable Bluetooth optical reader enables intermittent or continuous measurements. Proof of concept for applications in high altitude, exercise physiology, vascular disease, stroke, tumors, and other disease states have been shown in mouse, rat and porcine models. Over 90 sensors have been studied to date in humans. These novel tissueintegrating sensors yield real-time insights in tissue oxygen fluctuations for research and clinical applications.

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Oxygen Distributions in Tissue Measured by Phosphorescence Quenching

Cited by 22 publications

References 8 publications

Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke

Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke

Imaging the Hypoxic Tumor Microenvironment in Preclinical Models

Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia

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