Oxytocin mediates neuroprotection against hypoxic-ischemic injury in hippocampal CA1 neuron of neonatal rats

Wu, Zhihong; Xie, Changning; Kuang, Haixia; Wu, Jian; Chen, Xiao; Liu, Huibao; Liu, Tao

doi:10.1016/j.neuropharm.2021.108488

Cited by 15 publications

(16 citation statements)

References 57 publications

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“…On the AHP, SAM decreased the amplitude (9.01 ± 0.61 mV in CTR vs. 8.20 ± 0.81 mV in SAM, n = 12, p = 0.044 by paired t test; shown in Fig. 2Cb), but did not affect the FWHM (35.74 ± 4.51 ms in CTR vs. 35.51 ± 3.38 ms in SAM, n = 12, p = 0.926 by paired t test; shown in Fig. 2Cc).…”

Section: Effect Of Sam On the Electrical Activity Of Ot Neuronsmentioning

confidence: 89%

Excitatory Effects of Astrocytic Hydrogen Sulfide on the Electrical Activity of Oxytocin Neurons in the Supraoptic Nucleus

et al. 2022

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Introduction: In the regulation of oxytocin (OT) neuronal activity, hydrogen sulfide (H2S), a gaseous neurotransmitter, likely exerts an excitatory role. This role is associated with increased expression of astrocytic cystathionine -β- synthase (CBS), the key enzyme for H2S synthesis. However, it remains unclear whether H2S is mainly produced in astrocytes and contributes to the autoregulation of OT neurons. Methods: In hypothalamic slices of male rats, OT and H2S-associated drug effects were observed on the firing activity and spontaneous excitatory postsynaptic currents (sEPSCs) of putative OT neurons in the supraoptic nucleus (SON) in whole-cell patch-clamp recording. Expression of glial fibrillary acidic protein (GFAP) in the SON was analyzed in Western blots. In addition, changes in the length of rat pups’ hypothalamic astrocyte processes were observed in primary cultures. Results: In brain slices, OT significantly increased the firing rate of OT neurons, which was simulated by CBS allosteric agonist S-adenosyl-L-methionine (SAM) and H2S slow-releasing donor GYY4137 and blocked by CBS inhibitor aminooxyacetic acid (AOAA). L-α-aminoadipic acid (L-AAA, a gliotoxin) blocked SAM-evoked excitation. OT and SAM also increased the frequency and amplitude of sEPSCs; the effect of OT was blocked by AOAA. Both OT and GYY4137 reduced GFAP expression in the SON. Morphologically, OT or GYY4137 time-dependently reduced the length of astrocyte processes in primary cultures. Conclusions: These findings indicate that the auto-excitatory effect of OT on OT neurons is mediated by H2S from astrocytes at least partially and astrocytic H2S can elicit retraction of astrocyte processes that subsequently increase OT neuronal excitability.

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Section: Effect Of Sam On the Electrical Activity Of Ot Neuronsmentioning

confidence: 89%

Excitatory Effects of Astrocytic Hydrogen Sulfide on the Electrical Activity of Oxytocin Neurons in the Supraoptic Nucleus

et al. 2022

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“…Acute coronal brain slices for electrophysiology were prepared as described in our previous study (Wu et al, 2021 ). Mice were deeply anesthetized with urethane and transcardially perfused with 20 ml ice-cold carbonated (95% O 2 and 5% CO 2 ) dissection solution containing (mM): 240 sucrose, 2.5 KCl, 3.5 MgCl 2 , 0.5 CaCl 2 , 1.25 NaH 2 PO 4 , 0.4 ascorbic acid, 2 sodium pyruvate, 25 NaHCO 3 , and 1 kynurenic acid.…”

Section: Methodsmentioning

confidence: 99%

Genetic Deficiency of p53 Leads to Structural, Functional, and Synaptic Deficits in Primary Somatosensory Cortical Neurons of Adult Mice

Kuang

Liu

Jiao

et al. 2022

Front. Mol. Neurosci.

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The tumor suppressor p53 plays a crucial role in embryonic neuron development and neurite growth, and its involvement in neuronal homeostasis has been proposed. To better understand how the lack of the p53 gene function affects neuronal activity, spine development, and plasticity, we examined the electrophysiological and morphological properties of layer 5 (L5) pyramidal neurons in the primary somatosensory cortex barrel field (S1BF) by using in vitro whole-cell patch clamp and in vivo two-photon imaging techniques in p53 knockout (KO) mice. We found that the spiking frequency, excitatory inputs, and sag ratio were decreased in L5 pyramidal neurons of p53KO mice. In addition, both in vitro and in vivo morphological analyses demonstrated that dendritic spine density in the apical tuft is decreased in L5 pyramidal neurons of p53KO mice. Furthermore, chronic imaging showed that p53 deletion decreased dendritic spine turnover in steady-state conditions, and prevented the increase in spine turnover associated with whisker stimulation seen in wildtype mice. In addition, the sensitivity of whisker-dependent texture discrimination was impaired in p53KO mice compared with wildtype controls. Together, these results suggest that p53 plays an important role in regulating synaptic plasticity by reducing neuronal excitability and the number of excitatory synapses in S1BF.

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“…Moreover, perinatal oxytocin impact on neurodevelopment can be attributed to different factors. First, perinatal oxytocin contributes to the shift of GABAergic signalling to inhibitory before birth in rodents and thereby it modulates excitatory/inhibitory balance [ 155 , 156 ]. Second, oxytocin confers beneficial activities in maturation, neurogenesis, and synaptogenesis of hippocampus neurons in the newborn [ 156 , 157 ].…”

Section: Endogenous Neuromodulators and Pharmacological Agents In Bra...mentioning

confidence: 99%

Role of Brain Modulators in Neurodevelopment: Focus on Autism Spectrum Disorder and Associated Comorbidities

et al. 2022

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Autism spectrum disorder (ASD) and associated neurodevelopmental disorders share similar pathogenesis and clinical features. Pathophysiological changes in these diseases are rooted in early neuronal stem cells in the uterus. Several genetic and environmental factors potentially perturb neurogenesis and synaptogenesis processes causing incomplete or altered maturation of the brain that precedes the symptomology later in life. In this review, the impact of several endogenous neuromodulators and pharmacological agents on the foetus during pregnancy, manifested on numerous aspects of neurodevelopment is discussed. Within this context, some possible insults that may alter these modulators and therefore alter their role in neurodevelopment are high-lighted. Sometimes, a particular insult could influence several neuromodulator systems as is supported by recent research in the field of ASD and associated disorders. Dopaminergic hy-pothesis prevailed on the table for discussion of the pathogenesis of schizophrenia (SCH), atten-tion-deficit hyperactivity disorder (ADHD) and ASD for a long time. However, recent cumulative evidence suggests otherwise. Indeed, the neuromodulators that are dysregulated in ASD and comorbid disorders are as diverse as the causes and symptoms of this disease. Additionally, these neuromodulators have roles in brain development, further complicating their involvement in comorbidity. This review will survey the current understanding of the neuromodulating systems to serve the pharmacological field during pregnancy and to minimize drug-related insults in pa-tients with ASD and associated comorbidity disorders, e.g., SCH or ADHD.

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Oxytocin mediates neuroprotection against hypoxic-ischemic injury in hippocampal CA1 neuron of neonatal rats

Cited by 15 publications

References 57 publications

Excitatory Effects of Astrocytic Hydrogen Sulfide on the Electrical Activity of Oxytocin Neurons in the Supraoptic Nucleus

Excitatory Effects of Astrocytic Hydrogen Sulfide on the Electrical Activity of Oxytocin Neurons in the Supraoptic Nucleus

Genetic Deficiency of p53 Leads to Structural, Functional, and Synaptic Deficits in Primary Somatosensory Cortical Neurons of Adult Mice

Role of Brain Modulators in Neurodevelopment: Focus on Autism Spectrum Disorder and Associated Comorbidities

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