p-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells

Abstract

Abnormal glycosylation of cancer cells is considered a key factor of carcinogenesis related to growth, proliferation, migration and invasion of tumor cells. Many plant-based polyphenolic compounds reveal potential anti-cancer properties effecting cellular signaling systems. Herein, we assessed the effects of phenolic acid, p-coumaric acid and flavonoids such as kaempferol, astragalin or tiliroside on expression of selected cancer-related glycoforms and enzymes involved in their formation in AGS gastric cancer cells. The cells were treated with 80 and 160 µM of the compounds. RT-PCR, Western blotting and ELISA tests were performed to determine the influence of polyphenolics on analyzed factors. All the examined compounds inhibited the expression of MUC1, ST6GalNAcT2 and FUT4 mRNAs. C1GalT1, St3Gal-IV and FUT4 proteins as well as MUC1 domain, Tn and sialyl T antigen detected in cell lysates were also lowered. Both concentrations of kaempferol, astragalin and tiliroside also suppressed ppGalNAcT2 and C1GalT1 mRNAs. MUC1 cytoplasmic domain, sialyl Tn, T antigens in cell lysates and sialyl T in culture medium were inhibited only by kaempferol and tiliroside. Nuclear factor NF-κB mRNA expression decreased after treatment with both concentrations of kaempferol, astragalin and tiliroside. NF-κB protein expression was inhibited by kaempferol and tiliroside. The results indicate the rationality of application of examined polyphenolics as potential preventive agents against gastric cancer development.

Anticancer applications of phytochemicals in gastric cancer: Effects and molecular mechanism

“…Scutellarin suppressed GC by altering lactate dehydrogenase profile, DNA Frontiers in Pharmacology frontiersin.org density, mucus content and acidity (Sun and Meng, 2022). Kaempferol, p-Coumaric acid, Astragalin and Tiliroside influence abnormal glycosylation of GC cells, so as to exert the anticancer effect (Radziejewska et al, 2022). DIM suppressed GC via mediated ferroptosis, store-operated calcium entry, gastric cancerderived mesenchymal stem cells, endogenous hydrogen sulfide biosynthesis (Ye et al, 2020;Ye et al, 2021b;Shi et al, 2021;Ye et al, 2022).…”

A study related to the treatment of gastric cancer with Xiang-Sha-Liu-Jun-Zi-Tang based on network analysis

“…It can inhibit the cell cycle of GC cells, promote autophagy of GC cells. It has therapeutic potential for anti-GC effects [ 28 , 29 ]. In conclusion, the above studies confirm that XSLJZT, as a compound of traditional Chinese medicine, contains major active ingredients that have significant inhibitory effects on GC.…”

Astragalin: a food-origin flavonoid with therapeutic effect for multiple diseases

“…Specifically, Radziejewska et al observed that AG blocked the synthesis of Tn antigen, sialyl Tn antigen, and T antigen via suppressing the expressions of the glycosyltransferases ppGalNAcT2, ST6GalNAcT2, C1GalT1, St3Gal-IV, and FUT4 in AGS cells. Moreover, AG also decreased the level of NF-κB, which suggests that the NF-κB signaling pathway might be involved in the inhibition of the glycosylation process ( Radziejewska et al, 2022 ). In addition, AG was reported to induce the death of gastric cancer cells and reduce cancer cell viability in a gastric cancer cell-based xenograft mouse without obvious toxicity.…”