PACAP-mediated sperm–cumulus cell interaction promotes fertilization

Tanii, Ichiro; Aradate, Tadashi; Matsuda, Kouhei; Komiya, Akira; Fuse, Hideki

doi:10.1530/rep-10-0201

Cited by 44 publications

(43 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, multiple logistic regression analysis revealed that curvilinear velocity and ALH were the independent variables to predict % LNV. ALH was recently reported to be significantly increased in association with the fertilization rate promoted by pituitary adenylate cyclase-activating polypeptide (PACAP)-mediated sperm-cumulus cell interaction [Tanii et al 2011].…”

Section: Discussionmentioning

confidence: 99%

“…The definition of male infertility was the failure to conceive following twelve months of unprotected intercourse due to possible male factors. Semen samples were evaluated within one hour of collection by conventional semen analyses [WHO 1999] and SMAS (Kaga Electronics, Tokyo, Japan), which were performed as described previously [Akashi et al 2010;Tanii et al 2011]. In addition, spermatozoa were analyzed at 3,700x or 6,150x magnification by an inverted microscope equipped with Nomarski differential interference contrast optics (IX71, Olympus, Tokyo) and a video system (FX630, Olympus, Tokyo).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Komiya

Watanabe

Kawauchi

et al. 2012

Systems Biology in Reproductive Medicine

Self Cite

View full text Add to dashboard Cite

This study compared the sperm nuclear vacuoles and semen quality in the evaluation of male infertility. One hundred and forty-two semen samples were obtained from patients who visited the Male Infertility Clinic at Toyama University Hospital. Semen samples were evaluated by conventional semen analyses and the Sperm Motility Analysis System (SMAS). In addition, spermatozoa were analyzed at 3,700-6,150x magnification on an inverted microscope equipped with DIC/Nomarski differential interference contrast optics. A large nuclear vacuole (LNV) was defined as one or more vacuoles with the maximum diameter showing > 50% width of the sperm head. The percentage of spermatozoa with LNV (% LNV) was calculated for each sample. Correlations between the % LNV and parameters in SMAS and conventional semen analyses were analyzed. Processed motile spermatozoa from each sample were evaluated. The mean age of patients was 35 years old. Semen volume was 2.9 ± 1.6mL (0.1-11.0; mean ± standard deviation, minimum-maximum), sperm count was 39.3 ± 54.9 (x10 6 /mL, 0.01-262.0), sperm motility was 25.1 ± 17.8% (0-76.0), and normal sperm morphology was 10.3 ± 10.1% (0-49.0). After motile spermatozoa selection, we could evaluate % LNV in 125 ejaculates (88.0%) and at least one spermatozoon with LNV was observed in 118 ejaculates (94.4%). The percentage of spermatozoa with LNV was 28.0 ± 22.4% (0-100) and % LNV increased significantly when semen quality decreased. The correlation between the % LNV and the semen parameters was weak to moderate; correlation coefficients were -0.3577 in sperm count ( p < 0.0001), -0.2368 in sperm motility ( p = 0.0084), -0.2769 in motile sperm count ( p = 0.019), -0.2419 in total motile sperm count ( p = 0.0070), and -0.1676 in normal sperm morphology ( p = 0.0639). The % LNV did not show a significant correlation with the SMAS parameters except for weak correlation to beat/cross frequency (r = -0.2414, p = 0.0071). The percentage of spermatozoa with LNV did not have a strong correlation with parameters in conventional semen analysis and SMAS in the patients with male infertility; however, a certain level of negative influence of LNV to sperm quality cannot be excluded.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Komiya

Watanabe

Kawauchi

et al. 2012

Systems Biology in Reproductive Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the cumulus seems to be beneficial for fertilization (25)(26)(27). According to Toyoda et al (28), fertilization rates of cumulus-free oocytes in vitro are rather erratic, whereas those of cumulus-enclosed oocytes are high and consistent ( figure 30 of ref.…”

Section: According To Gahlay Et Al (5) Zp Of Transgenic Mice (Zp2mentioning

confidence: 99%

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization

Jin¹,

Fujiwara²,

Kakiuchi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

366

283

View full text Add to dashboard Cite

To fuse with oocytes, spermatozoa of eutherian mammals must pass through extracellular coats, the cumulus cell layer, and the zona pellucida (ZP). It is generally believed that the acrosome reaction (AR) of spermatozoa, essential for zona penetration and fusion with oocytes, is triggered by sperm contact with the zona pellucida. Therefore, in most previous studies of sperm-oocyte interactions in the mouse, the cumulus has been removed before insemination to facilitate the examination of sperm-zona interactions. We used transgenic mouse spermatozoa, which enabled us to detect the onset of the acrosome reaction using fluorescence microscopy. We found that the spermatozoa that began the acrosome reaction before reaching the zona were able to penetrate the zona and fused with the oocyte's plasma membrane. In fact, most fertilizing spermatozoa underwent the acrosome reaction before reaching the zona pellucida of cumulus-enclosed oocytes, at least under the experimental conditions we used. The incidence of in vitro fertilization of cumulus-free oocytes was increased by coincubating oocytes with cumulus cells, suggesting an important role for cumulus cells and their matrix in natural fertilization.sperm-egg interaction | acrosomal exocytosis | real-time imaging | video microscopy F ertilization is a critical moment in animal sexual reproduction and comprises sequential steps in the interaction of sperm with an egg. In eutherian mammals, natural fertilization is accomplished by sperm ascent to the oviduct ampulla, passage through the cumulus oophorus surrounding oocytes, loose binding to the oocyte's outer envelope, the zona pellucida (ZP), followed by tight sperm binding to the ZP and penetration through the ZP, culminating in sperm-oocyte membrane fusion (1). Before penetrating into the ZP, the fertilizing spermatozoon must undergo a morphological change involving disruption of the acrosome called the acrosome reaction (AR). In most studies of mouse sperm-oocyte interactions in vitro, the cumulus oophorus is removed by hyaluronidase treatment before insemination, allowing direct sperm contact with the ZP upon insemination. However, under natural in vivo conditions, spermatozoa first encounter the cumulus before reaching the ZP surface. The question this paper addresses is: Where does the fertilizing mouse spermatozoon begin the AR-in the cumulus or at the ZP?In the mouse, it has been reported that a ZP glycoprotein, ZP3, has both sperm-binding (sperm receptor) and AR-inducing activities and is essential for formation of the zona pellucida and therefore fertility (2, 3). The specificity of sperm binding to homologous zona might be conferred by the supramolecular structure (reviewed in ref. 4) or cleavage status of ZP glycoproteins (5). It is generally assumed that-at least in the mouse-the AR occurs after sperm binding to the ZP (6, 7). Even though spermatozoa of several species other than the mouse are known to be able to undergo the AR on the ZP surface (8-10), the presence of motile spermatozoa within the cumulus at...

show abstract

“…However this model cannot explain why quite a number of spermatozoa in the cumulus oophorus are already acrosome-reacted, before they get to ZP (Bedford, 1972;Cummins and Yanagimachi, 1982). Recently, accumulating evidence of cross-talk between the cumulus oophorus and spermatozoa has emerged (Yin et al, 2009;Tanii et al, 2011), suggesting a more important role of the cumulus matrix and cumulus cells in fertilization, which has been long overlooked. Herein we re-examine evidences related to AR in the cumulus oophorus, taking into account of the recent discovery of a sperm factor, NYD-SP8, released upon sperm-cumulus interaction, and discuss the molecular basis underlying the interaction of sperm and the cumulus and its potential physiological significance in fertilization.…”

Section: Introductionmentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

View full text Add to dashboard Cite

Fertilization is a process involving multiple steps that lead to the final fusion of one sperm and the oocyte to form the zygote. One of the steps, acrosome reaction (AR), is an exocytosis process, during which the outer acrosome membrane fuses with the inner sperm membrane, leading to the release of acrosome enzymes that facilitate sperm penetration of the egg investments. Though AR has been investigated for decades, the initial steps of AR in vivo, however, remain largely unknown. A well elucidated model holds the view that AR occurs on the surface of the zona pellucida (ZP), which is triggered by binding of sperm with one of the ZP glycosylated protein, ZP3. However, this model fails to explain the large number of 'falsely' acrosome-reacted sperms found within the cumulus layer in many species examined. With the emerging evidence of cross-talk between sperm and cumulus cells, the potential significance of AR in the cumulus oophorus, the outer layer of the egg, has been gradually revealed. Here we review the acrosome status within the cumulus layer, the cross-talk between sperm and cumulus cells with the involvement of a novel spermreleased factor, NYD-SP8, and re-evaluate the importance and physiological significance of the AR in the cumulus in fertilization.

show abstract

PACAP-mediated sperm–cumulus cell interaction promotes fertilization

Cited by 44 publications

References 44 publications

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Contact Info

Product

Resources

About