Paenibacillus relictisesami sp. nov., isolated from sesame oil cake

Abstract

A facultatively anaerobic, Gram-stain-positive, rod-shaped bacterium, designated strain KB0549 T , was isolated from sesame oil cake. Cells were motile, round-ended rods, and produced central or terminal spores. The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid. The major fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The DNA G+C content of strain KB0549 T was 51.9 mol%. On the basis of 16S rRNA gene sequence phylogeny, strain KB0549 T was affiliated with the genus Paenibacillus in the phylum Firmicutes and was most closely related to Paenibacillus cookii with 97.4 % sequence similarity. Strain KB0549 T was physiologically differentiated from P. cookii by the high content of anteiso-C 17 : 0 , inability to grow at 50 6C, spore position, and negative Voges-Proskauer reaction. Based on these unique physiological and phylogenetic characteristics, it is proposed that the isolate represents a novel species, Paenibacillus relictisesami sp. nov.; the type strain is KB0549 T (5JCM 18068 T 5DSM 25385 T ). Ash et al. (1993) proposed that 11 strains belonging to 'Group 3' within the genus Bacillus should be transferred to the genus Paenibacillus. The descriptions of Paenibacillus amylolyticus, Paenibacillus illinoisensis and Paenibacillus chibensis as species within the genus Paenibacillus were later amended by Shida et al. (1997). At the time of writing, the genus Paenibacillus contains 147 species and four subspecies (http://www.bacterio.net/p/paenibacillus.html).Members of the genus Paenibacillus are aerobic or facultatively anaerobic, rod-shaped, endospore-forming bacteria. Species of this genus have been isolated from various sources, including soil (Yoon et al., 2007), rhizosphere (Daane et al., 2002;Kuisiene et al., 2008), compost (Iida et al., 2005Ueda et al., 2013), warm springs (Saha et al., 2005) and human faeces (Hoyles et al., 2012). Several of the characterized strains have useful enzymic activities; for example, Paenibacillus sp. JDR-2 produces xylanase and is, therefore, able to utilize hemicellulosic polysaccharides (St John et al., 2006). Members of the genus Paenibacillus contain meso-diaminopimelic acid as the major diamino acid in peptidoglycan, and have anteiso-C 15 : 0 as the major cellular fatty acid. The DNA G+C content of species ranges from 36 to 59 mol% (Ash et al., 1993;Shida et al., 1997;De Vos et al., 2009). In this communication, we describe the polyphasic taxonomic characterization of a sesaminol-producing bacterium, strain KB0549 T , which was isolated from sesame oil cake (SOC) and was determined to be affiliated with the genus Paenibacillus.Sesaminol is one of several lignans found in sesame oil, having strong antioxidant activity as well as a variety of health benefits to humans (Fukuda et al., 1986;Kang et al., 1998;Kumazawa et al., 2003). Sesame seeds contain large amounts of sesaminol in glycosidic form. Sesaminolis the most abundant lignan in sesame seeds and is therefore a potentially important source of sesaminol. However, due to its bra...

Paenibacillus oenotherae sp. nov. and Paenibacillus hemerocallicola sp. nov., isolated from the roots of herbaceous plants

“…The diamino acid in the peptidoglycan is meso -diaminopimelic acid, and the major cellular fatty acid is anteiso-C 15 : 0 (Ash et al , 1993; Shida et al , 1997). Novel species of the genus have been isolated recently from various sources, including compost (Ueda et al , 2013), food (Shimoyama et al , 2014), lake water (Wu et al , 2013), root nodules (Carro et al , 2013, 2014), plants (Kong et al , 2013; Tonouchi et al , 2014), the rhizosphere (Son et al , 2014; Zhang et al , 2013), rock (Guo et al , 2014), sediment (Lee et al , 2013) and soil (Dsouza et al , 2014; Li et al , 2014a, b; Xiang et al , 2014; Yao et al , 2014).…”

Paenibacillus enshidis sp. nov., Isolated from the Nodules of Robinia pseudoacacia L.

“…Ellipsoidal endospores are formed and genomic DNA G?C contents range from 39 to 54 mol % [15,19]. There were more than 150 species of this genus (http://www.bacterio.…”

Paenibacillus tibetensis sp. nov., a psychrophilic bacterium isolated from alpine swamp meadow soil

“…(http://www.bacterio.net/paenibacillus.html). Among all the species, most species are either mesophilic (Enright et al , 2003; Kim et al , 2014; Lim et al , 2006; Saha et al , 2005) or thermophilic (Li et al , 2014; Shimoyama et al , 2014; Yao et al , 2014), with the exception of three psychrophilic species, Paenibacillus macquariensis (Marshall & Ohye, 1966), Paenibacillus glacialis (Kishore et al , 2010) and Paenibacillus darwinianus (Dsouza et al , 2014). In this study, we isolated a psychrophilic strain, SSB001 T , closely related to these species, which belongs to a novel species of genus Paenibacillus for which we propose the name Paenibacillus tibetensis sp.…”