Paeoniflorin suppresses inflammatory mediator production and regulates G protein-coupled signaling in fibroblast – like synoviocytes of collagen induced arthritic rats

Zhang, L.-L.; Wei, Wen-mei; Wang, N.-P.; Wang, Q.-T.; Chen, Jing Yu; Chen, Y.; Wu, Hua; Hu, Xueyang

doi:10.1007/s00011-007-7240-x

Cited by 101 publications

(63 citation statements)

References 19 publications

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“…no. 12-1001, HPLC Ն 98%) was obtained from Shanghai R&D Center for Standardization of Traditional Chinese Medicine, Shanghai, China, and the dosage selection (50 mg/kg) was based on previous in vivo studies and was confirmed to result in effective alleviation of acute lung injury and arthritis in mouse disease models (40,43).…”

Section: Methodsmentioning

confidence: 99%

“…A decoction of the dried root of peony has been used in the treatment of rheumatoid arthritis in China for more than 1,200 years (13,22). Paeoniflorin, a monoterpene glucoside, is the principal bioactive constituent of peony root (40). Paeoniflorin has reportedly exhibited anti-inflammation, immunoregulation, antiarthritis, antihepatitis, pain-relieving, neuromuscular blocking, and antihyperglycemia effects (15,32,40,41).…”

mentioning

confidence: 99%

“…Paeoniflorin, a monoterpene glucoside, is the principal bioactive constituent of peony root (40). Paeoniflorin has reportedly exhibited anti-inflammation, immunoregulation, antiarthritis, antihepatitis, pain-relieving, neuromuscular blocking, and antihyperglycemia effects (15,32,40,41). Previous studies have shown that paeoniflorin suppresses TNF-␣ induced chemokine production in human dermal microvascular endothelial cells by blocking the NF-B and ERK pathway (2).…”

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confidence: 99%

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Paeoniflorin abrogates DSS-induced colitis via a TLR4-dependent pathway

Zhang

Dou

Zhang

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

112

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Paeonia lactiflora Pall is one of the most well-known herbs in China, Korea, and Japan for more than 1,200 years. Paeoniflorin, the major bioactive component of peony root, has recently been reported to have anticolitic activity. However, the underlying molecular mechanism is unclear. The present study was to explore the possible mechanism of paeoniflorin in attenuating dextran sulfate sodium (DSS)-induced colitis. Pre- and coadministration of paeoniflorin significantly reduced the severity of colitis and resulted in downregulation of several inflammatory parameters in the colon, including the activity of myeloperoxidase (MPO), the levels of TNF-α and IL-6, and the mRNA expression of proinflammatory mediators (MCP-1, Cox2, IFN-γ, TNF-α, IL-6, and IL-17). The decline in the activation of NF-κB p65, ERK, JNK, and p38 MAPK correlated with a decrease in mucosal Toll-like receptor 4 (TLR4) but not TLR2 or TLR5 expression. In accordance with the in vivo results, paeoniflorin downregulated TLR4 expression, blocked nuclear translocation of NF-κB p65, and reduced the production of IL-6 in LPS-stimulated mouse macrophage RAW264.7 cells. Transient transfection assay performed in LPS-stimulated human colon cancer HT-29 cells indicated that paeoniflorin inhibits NF-κB transcriptional activity in a dose-dependent manner. TLR4 knockdown and overexpression experiments demonstrated a requirement for TLR4 in paeoniflorin-mediated downregulation of inflammatory cytokines. Thus, for the first time, the present study indicates that paeoniflorin abrogates DSS-induced colitis via decreasing the expression of TLR4 and suppressing the activation of NF-κB and MAPK pathways.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Paeoniflorin abrogates DSS-induced colitis via a TLR4-dependent pathway

Zhang

Dou

Zhang

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

112

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“…PF has many biological activities and has been shown to be anti-inflammatory [2][3][4] , analgesic [5] , and an antioxidant [6,7] . Furthermore, PF is a potential neuroprotective agent.…”

Section: Introductionmentioning

confidence: 99%

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Zhong

Song

et al. 2015

Acta Pharmacol Sin

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Aim: Paeoniflorin from Chinese herb Paeoniae Radix has been shown to ameliorate middle cerebral artery occlusion-induced ischemia in rats. The aim of this study was to investigate the mechanisms underlying the neuroprotective action of PF in cultured rat cortical neurons. Methods: Primary cultured cortical neurons of rats were subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) insult. Cell survival was determined using MTT assay. HEK293 cells stably transfected with A 1 R (HEK293/A 1 R) were used for detailed analysis. Phosphorylation of the signaling proteins was evaluated by Western blot or immunoprecipitation. Receptor interactions were identified using co-immunoprecipitation and immunofluorescence staining. Results: Paeoniflorin (10 nmol/L to 1 μmol/L) increased the survival of neurons subjected to OGD/R. Furthermore, paeoniflorin increased the phosphorylation of Akt and ERK1/2 in these neurons. These effects were blocked by PI3K inhibitor wortmannin or MEK inhibitor U0126. Paeoniflorin also increased the phosphorylation of Akt and ERK1/2 in HEK293/A 1 R cells. Both A 1 R antagonist DPCPX and EGFR inhibitor AG1478 not only blocked paeoniflorin-induced phosphorylation of ERK1/2 and Akt in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. In addition, paeoniflorin increased the phosphorylation of Src kinase and activation of MMP-2 in HEK293/A 1 R cells. Both Src inhibitor PP2 and MMP-2/MMP-9 inhibitor BiPs not only blocked paeoniflorininduced phosphorylation of ERK1/2 (and Akt) in HEK293/A 1 R cells, but also paeoniflorin-increased survival of neurons subjected to OGD/R. Conclusion: Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A 1 Rmediated transactivation of EGFR.

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“…Reversedphase high-performance liquid chromatography (HPLC) has indicated that TGP contain eight principal components, namely paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, oxybenzoyl-paeoniflorin, albiflorin, paeoniflorigenone, and lactiflorin (Figure 1 (18). TGP are considered to have antiinflammatory, antioxidative, hepatoprotective, and immunoregulatory actions (19)(20)(21). TGP are a diseasemodifying medication for rheumatoid arthritis that was approved by the State Food and Drug Administration (SFDA) in 1998.…”

Section: Introductionmentioning

confidence: 99%

TGP attenuates endoplasmic reticulum stress and regulates the expression of thioredoxin-interacting protein in the kidneys of diabetic rats

Shao

et al. 2016

BST

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Paeoniflorin suppresses inflammatory mediator production and regulates G protein-coupled signaling in fibroblast – like synoviocytes of collagen induced arthritic rats

Cited by 101 publications

References 19 publications

Paeoniflorin abrogates DSS-induced colitis via a TLR4-dependent pathway

Paeoniflorin abrogates DSS-induced colitis via a TLR4-dependent pathway

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

TGP attenuates endoplasmic reticulum stress and regulates the expression of thioredoxin-interacting protein in the kidneys of diabetic rats

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