Paeonol produced by Chaetomium sp., an endophytic fungus isolated from Paeonia suffruticosa

Yang, Guodong; Qiu, Yan; Lin, Lin; Dong, Fengxia

doi:10.1016/j.phytol.2015.07.019

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…The reference standards of gallic acid, 5‐HMF, oxypaeoniflora, paeoniflorin, benzoic acid, methyl paraben, quercetin and 3‐hydroxy‐4‐methoxyacetophenone (internal standard, IS) were all purchased from Chroma Biotechnology Co. Ltd (purity ≥98%; Chengdu, China). The reference standards of 3,8‐dihydroxy‐2‐methylchromone and paeonol were isolated and purified by our laboratory (purity ≥98%), and their structures were elucidated by comparing their spectroscopic data (ESI‐MS, 1 H‐NMR and 13 C‐NMR, HMBC, HMQC and H–H COSY) in the literature (Li, Yang, Qiu, Lin, & Dong, 2015; Popoff & Theander, 1977; Wang et al, 2011). All of the structures of the standards are listed in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Nine absorbed components pharmacokinetic of raw and processed Moutan Cortex in normal and blood‐heat and hemorrhage syndrome model rats

Cheng

Yan

et al. 2020

Biomedical Chromatography

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Raw Moutan Cortex (RMC) and Processed Moutan Cortex (PMC) have a long history of use in China and other Asian countries. In this study, a rapid and accurate ultra‐high‐pressure liquid chromatography coupled with diode array detector (UHPLC–DAD) method was developed and validated for the simultaneous determination of nine absorbed compounds of RMC/PMC. After extraction by protein precipitation with methanol from plasma, the analytes were separated on an Acquity UPLC® BEH Shield RP18 column (2.1 × 100 mm, 1.7 μm, Waters, USA). Acetonitrile (A) and 0.1% (v/v) formic acid in water (B) were selected as the mobile phase to perform gradient elution. The linearity of nine analytes was >0.9915. The intra‐ and inter‐assay precision (RSD) values were within 11.18%, and accuracy ranged from 91.32 to 101.29%. Suitable stability, matrix effect and extraction recoveries were also obtained. The validated method was applied to compare the pharmacokinetics of RMC and PMC in Blood‐Heat and Hemorrhage Syndrome Model and normal rats. The results revealed that processing and the pathological state could influence the pharmacokinetic characteristics of compounds in RMC/PMC. The study willbe useful for further studies on pharmacokinetics and clinical application of raw and processed Moutan Cortex.

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Section: Methodsmentioning

confidence: 99%

Nine absorbed components pharmacokinetic of raw and processed Moutan Cortex in normal and blood‐heat and hemorrhage syndrome model rats

Cheng

Yan

et al. 2020

Biomedical Chromatography

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“…A total of 30 individuals of each kind of tree peonies were collected. All plant materials were immediately brought to the laboratory, stored at 4 °C in refrigerator and preprocessed within 24 h. The tree peonies endophytic fungi were isolated according to the surface sterilization method described by Li Peng et al 15 …”

Section: Methodsmentioning

confidence: 99%

Diversity and communities of culturable endophytic fungi from different tree peonies (geoherbs and non-geoherbs), and their biosynthetic potential analysis

Yang

Meng

et al. 2018

Brazilian Journal of Microbiology

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To know more about the potential roles of endophytic fungi in the formation mechanism of Daodi medicinal material, diversity and communities of culturable endophytic fungi in three types of tree peonies were investigated. Endophytic fungi of three types of tree peonies were isolated and identified. The diversity was analyzed. Bayesian trees constructed by MrBayes 3.2.6 after phylogenetic analysis of the ITS sequences. The endophytic fungi potential for synthesis of natural products was assessed by means of detecting NRPS and PKS gene sequences. In total, 364 endophytic fungi isolates representing 26 genera were recovered from Paeonia ostii 'Feng Dan', Paeonia ostii 'Luoyang Feng Dan', and Paeonia suffruticosa 'Luoyang Hong'. More culturable endophytic fungi appeared in P. suffruticosa 'Luoyang Hong' (206) compared with P. ostii 'Feng Dan' (60) and P. ostii 'Luoyang Feng Dan' (98). The fungal community of P. ostii 'Feng Dan' had the highest richness and diversity. PKSs and NRPS detection rates of endophytic fungi from P. ostii 'Feng Dan' are both the highest among the three types of tree peonies. Results indicate that endophytic fungus is an important factor of Daodi Cortex Moutan forming, and endophytic fungi in peony are related to genuineness of Cortex Moutan.

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“…Ten of them were used as the representative target to study the molecular mechanism of 27 ligands of Chaetomium fungal endophyte from diverse plants [21][22][23][24][25][26][27]. On the other hand, the proteins being used as the target are methylerythritol phosphate cytidyltransferase (PDB 1I52) [28], maltose binding periplasmic protein (MBP; PDB 1JVY) [29], glutamine-fructose-6-phosphate aminotransferase (PDB 1MOS) [30], -aminobutyrate aminotransferase (PDB 1SZS) [31], DNA gyrase (PDB 4DUH) [32] , enoylreductase (PDB 1I30) [33] , isoleucyl t-RNA synthetase (PDB 1JZQ) [34], aspartate aminotransferase (PDB 1IX6) [35], acetylglutamate kinase (PDB 1OHA) [36], and peptidyl t-RNA hydroxylase (PDB 2PTH) [37].…”

Section: In Silico Activity Against Protein Targets In E Colimentioning

confidence: 99%

Antimicrobial, antioxidant, and cytotoxic activities of endhopitic fungi Chaetomium sp. isolated from Phyllanthus niruri Linn: in vitro and in silico studies

Rollando

Notario

Monica

et al. 2017

J. Pure App. Chem. Res.

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Endophytic fungi Chaetomium sp isolated from Phyllanthus niruri Linn. Mycelium powder was extracted by using ethyl acetate. Extract was fractionated using n-hexane, dichloromethane and ethanol 96%. The antimicrobial test was carried out using disc diffusion and microdilution methods. The antioxidant activity of the fraction was determined using hydrogen peroxide free radical scavenging and reducing power capacity activities. The cytotoxicity assay of the fraction against T47D breast cancer cell was carried out using dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide method (MTT). The in silico prediction of chemical substances which are reported exist in Chaetomium sp. performed using AutoDockVina embedded in PyRx version 8.0. Dichloromethane fraction was found as the most active sample against Escherichia coli (IC 50 20.76 g/mL), Staphylococcus aureus (IC 50 70.15 g/mL), Salmonella typhi (49.13 g/mL) and was found as the most high phenolic content with value 47.44 mg GAE/g fraction, whereas the best antioxidant activity was performed by ethanol 96% fraction (85%). Cytotoxicity assay against T47D cell line showed dichloromethane fraction have highest activity with IC 50 10.76 g/mL. The docking studies showed that compounds bearing xanthone structure were potential for maltose binding periplasmic and human aromatase associating with their potencies as antibacteria and anticancer. Endophytic fungi Chaetomium sp. was isolated from Phyllanthus niruri using n-hexane, dichloromethane and ethanol fractions was studied its various biological activities as antimicrobial, antioxidant and cytotoxic agent against breast cancer cell.

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Paeonol produced by Chaetomium sp., an endophytic fungus isolated from Paeonia suffruticosa

Cited by 9 publications

References 31 publications

Nine absorbed components pharmacokinetic of raw and processed Moutan Cortex in normal and blood‐heat and hemorrhage syndrome model rats

Nine absorbed components pharmacokinetic of raw and processed Moutan Cortex in normal and blood‐heat and hemorrhage syndrome model rats

Diversity and communities of culturable endophytic fungi from different tree peonies (geoherbs and non-geoherbs), and their biosynthetic potential analysis

Antimicrobial, antioxidant, and cytotoxic activities of endhopitic fungi Chaetomium sp. isolated from Phyllanthus niruri Linn: in vitro and in silico studies

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