Pancreatic Lipase

Brockman, Howard L.

doi:10.1007/978-1-4615-1195-3_4

Cited by 7 publications

(9 citation statements)

References 104 publications

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“…This is probably due to the larger surface and exposure of more exposed hydrophobic groups of the lipase/co-lipase complex compared with lipase alone. It is known that lipase is a rather hydrophilic protein, while co-lipase is hydrophobic [26][27][28][29][30][31]. The binding of lipase and the lipase/co-lipase complex to thylakoid probably involves both ionic and hydrophobic interactions.…”

Section: Binding Of Lipase To Thylakoid Membranesmentioning

confidence: 99%

“…During intestinal fat digestion, however, dietary lipids are dispersed in bile salts, and in this situation co-lipase is obligatory for lipolysis by anchoring the lipase/co-lipase complex to the lipid/water interface. This attachment involves hydrophobic as well as ionic interactions [26][27][28][29][30][31]. For reviews on the catalytic mechanism of lipase/co-lipase, see [30,31] and references therein.…”

Section: A Proposal For the Mechanism Of Inhibitionmentioning

confidence: 99%

“…This attachment involves hydrophobic as well as ionic interactions [26][27][28][29][30][31]. For reviews on the catalytic mechanism of lipase/co-lipase, see [30,31] and references therein. The conventional lipolysis system involves heterogeneous catalysis involving the lipase/co-lipase complex, bile salt and the surface of a lipid or micellar phase.…”

Section: A Proposal For the Mechanism Of Inhibitionmentioning

confidence: 99%

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Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase

Albertsson¹,

Köhnke²,

Emek³

et al. 2007

Biochemical Journal

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Human obesity is a global epidemic, which causes a rapidly increased frequency of diabetes and cardiovascular disease. One reason for obesity is the ready availability of refined food products with high caloric density, an evolutionarily new event, which makes over-consumption of food inevitable. Fat is a food product with high caloric density. The mechanism for regulation of fat intake has therefore been studied to a great extent. Such studies have shown that, as long as fat stays in the intestine, satiety is promoted. This occurs through the fat-released peptide hormones, the best known being CCK (cholecystokinin), which is released by fatty acids. Hence, retarded fat digestion with prolonged time for delivery of fatty acids promotes satiety. Pancreatic lipase, together with its protein cofactor, co-lipase, is the main enzymatic system responsible for intestinal fat digestion. We found that biological membranes, isolated from plants, animals or bacteria, inhibit the lipase/co-lipase-catalysed hydrolysis of triacylglycerols even in the presence of bile salt. We propose that the inhibition is due to binding of lipase/co-lipase to the membranes and adsorption of the membranes to the aqueous/triacylglycerol interface, thereby hindering lipase/co-lipase from acting on its lipid substrate. We also found that chloroplast membranes (thylakoids), when added to refined food, suppressed food intake in rats, lowered blood lipids and raised the satiety hormones, CCK and enterostatin. Consequently, the mechanism for satiety seems to be retardation of fat digestion allowing the fat products to stay longer in the intestine.

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Section: Binding Of Lipase To Thylakoid Membranesmentioning

confidence: 99%

Section: A Proposal For the Mechanism Of Inhibitionmentioning

confidence: 99%

Section: A Proposal For the Mechanism Of Inhibitionmentioning

confidence: 99%

See 1 more Smart Citation

Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase

Albertsson¹,

Köhnke²,

Emek³

et al. 2007

Biochemical Journal

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show abstract

“…E-mail: verger@ibsm.cnrs-mrs.fr. 2 Abbreviations used: BHT, butylhydroxytoluene; CMC, critical micellar concentration; EDTA, ethylenediaminetetraacetic acid; FFA, free fatty acid; HPL, human pancreatic lipase; NaTDC, sodium taurodeoxycholate; OD, optical density; TLC, thin-layer chromatography; TLL, Thermomyces lanuginosa lipase; rHGL, recombinant human gastric lipase; THL, tetrahydrolipstatin; TAGs, triacylglycerols.…”

mentioning

confidence: 99%

“…2 Considerable efforts have been made to elucidate their role in dietary fat digestion (1)(2)(3)(4) and to describe in detail the molecular and kinetic events occurring during the catalytic process at lipid/water interfaces (5). Many biotechnological applications for lipases have also been described in the food, cosmetic, detergent, and pharmaceutical industries (6 -9).…”

mentioning

confidence: 99%