Panton–Valentine leukocidin (PVL)-positive methicillin-susceptible and resistant Staphylococcus aureus in Taiwan: identification of oxacillin-susceptible mecA-positive methicillin-resistant S. aureus

Chen, Feng-Jui; Hiramatsu, Kazumasa; Huang, I-Wen; Wang, Chen-Her; Lauderdale, Tsai‐Ling

doi:10.1016/j.diagmicrobio.2009.07.024

Cited by 30 publications

(30 citation statements)

References 32 publications

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“…Seventeen clinical MRSA strains from our previous study (5,7) were chosen for mecA gene sequencing, including one SCCmec II isolate, two SCCmec III isolates, six SCCmec IV isolates, and eight SCCmec V isolates (see Table 3). Additional SCCmec type IV and V clinical isolates were included because they exhibited more diverse oxacillin MIC values on the basis of Etest results.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…The epidemiological factors that have contributed to the dissemination of ST59 clones in Taiwan are not well understood. However, we recently identified a pvl-positive ST59-SCCmec V clone with borderline resistance to oxacillin that is the major contributor to a low-level oxacillin-resistant mecA-positive phenotype, which may provide a clue to ST59 prevalence (7,8).…”

mentioning

confidence: 99%

“…There is wide variation in the level of oxacillin resistance (MIC range, 1 to Ͼ256 g/ml) of mecA-positive ST59 isolates in Taiwan (7,8), but the reasons for this variation are unknown. The present study investigated the impact of polymorphisms in the mecA gene from four SCCmec types on the level of oxacillin resistance in an ST59 clinical S. aureus genetic background.…”

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confidence: 99%

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Role of the mecA Gene in Oxacillin Resistance in a Staphylococcus aureus Clinical Strain with a pvl -Positive ST59 Genetic Background

Chen

Wang

Chen

et al. 2014

Antimicrob Agents Chemother

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The most prevalent community-associated methicillin-resistant Staphylococcus aureus (C-MRSA) strains in Taiwan, sequence type 59 (ST59) clones, carry staphylococcal cassette chromosome mec (SCCmec) type V and, to a lesser extent, type IV. These strains show wide variation in sensitivity to oxacillin, but the reasons for this variation are unknown. Here we compared the sequences of the mecA genes from clinical strains of different SCCmec types and found that they contain different mecA promoter mutations. Analysis of mecA promoter activity by reporter gene fusions showed that single base substitutions in the promoter have a strong influence on mecA transcription. The different mecA variants, including promoter sequences, were expressed in the methicillin-sensitive Staphylococcus aureus (MSSA) strain C195 (ST59 background). PBP 2a production among the parental strains and strains with promoter mutant mecA genes showed a close correlation with mecA transcription levels. Furthermore, the quantity of PBP 2a also closely correlated with the level of oxacillin resistance in the C195 background. Our data suggest that mecA promoter mutations play an important role in determining the level of oxacillin resistance. The mecA promoter mutation G-25A (25 bases upstream of the mecA translation start site) was found to be associated with a high oxacillin MIC (256 g/ml), G-7T conferred a moderate oxacillin MIC (32 to 64 g/ml), strains with C-33T showed a low oxacillin MIC (4 to 8 g/ml), and A-38G reversed the effect of the C-33T mutation, restoring the oxacillin resistance level in the A-38G C-33T double mutant. These observations may explain why C-MRSA strains in Taiwan carrying SCCmec type IV or V have such enormous variations in oxacillin MICs.

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Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Role of the mecA Gene in Oxacillin Resistance in a Staphylococcus aureus Clinical Strain with a pvl -Positive ST59 Genetic Background

Chen

Wang

Chen

et al. 2014

Antimicrob Agents Chemother

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“…The virulence-related genes encoding PVL, TSST-1, SEC, SEH, and ET were amplified using PCR based on methods provided in the literature (Chen et al, 2009;Kaltsas et al, 2011;Khokhlova et al, 2012;Zhao et al, 2012). The target genes were 433 bp for PVL, 350 bp for TSST-1, 257 bp for SEC, 375 bp for SEH, and 119 bp for ET.…”

Section: Virulence-related Gene Detectionmentioning

confidence: 99%

Epidemiological investigation of community-acquired Staphylococcus aureus infection

Zhou¹,

Zhang²,

Yu³

2013

Genet. Mol. Res.

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“…The predominant CA-MRSA clones in different regions have diverse genetic backgrounds, such as ST8 (USA300) in the United States, ST80 and ST45 in Europe, and ST30 in Asia (4,5,12,13), but most carry SCCmec IV, while ST59-SCCmec V ("Taiwan clone") predominates in Taiwan (3,8). We here report the complete genome of M013, a pvl-positive, ST59-SCCmec V MRSA strain isolated in 2002 from a wound specimen of a pediatric outpatient during part of the Taiwan Surveillance of Antimicrobial Resistance surveillance (TSAR) project (2,11).…”

mentioning

confidence: 99%

Complete Genome Sequence of Staphylococcus aureus M013, a pvl-Positive, ST59-SCCmec Type V Strain Isolated in Taiwan

Huang

Chen

Miu

et al. 2012

Journal of Bacteriology

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We report the complete genome sequence of M013, a representative strain of a pvl-positive, sequence type 59-staphylococcal cassette chromosome mec type V (ST59-SCCmec type V) community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clone in Taiwan. Comparison of M013 with the genomes of two CA-MRSA strains in the United States revealed major differences in the regions covering several genomic islands and prophages.

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Panton–Valentine leukocidin (PVL)-positive methicillin-susceptible and resistant Staphylococcus aureus in Taiwan: identification of oxacillin-susceptible mecA-positive methicillin-resistant S. aureus

Cited by 30 publications

References 32 publications

Role of the mecA Gene in Oxacillin Resistance in a Staphylococcus aureus Clinical Strain with a pvl -Positive ST59 Genetic Background

Role of the mecA Gene in Oxacillin Resistance in a Staphylococcus aureus Clinical Strain with a pvl -Positive ST59 Genetic Background

Epidemiological investigation of community-acquired Staphylococcus aureus infection

Complete Genome Sequence of Staphylococcus aureus M013, a pvl-Positive, ST59-SCCmec Type V Strain Isolated in Taiwan

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