Paper-based platform for detection by hybridization using intrinsically labeled fluorescent oligonucleotide probes on quantum dots

Abstract

Paper-based platform for the selective detection of DNA targets by hybridization using FRET between intrinsically labeled fluorescent DNA probes and quantum dots.

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

“…The paper-based sensor enabled genomic DNA's identification for Escherichia coli and Campylobacter jejuni, with the LOD of 2 × 10 3 copies/μL. Other μPads for nucleic acid detection [188][189][190][191][192] have been summarized in Table 2. Another important application for μPads is to directly detect cells.…”

Resonance Energy Transfer-Based Biosensors for Point-of-Need Diagnosis—Progress and Perspectives

“…(f) Paper-immobilized quantum dot-oligonucleotides and free Cy3-oligonucleotides contain different DNA segments complementary to the target gene fragment. In a sandwich format, the target gene serves as a hybridization bridge for the QD-oligonucleotide and Cy3-oligonucleotide, which in turn enables FRET between QD and Cy3 [84][85][86][87]; (g) A Cy3-labeled kanamycin-specific aptamer partially hybridizes to an anchor/connector oligonucleotide immobilized on glass. The connector oligonucleotide is conjugated to Cy5.…”

“…Krull and co-workers developed different sensing systems deployed at PON, using a paper-based design. The biosensors are based on the transduction of nucleic acid hybridization [84,85,87,91,92] and both a direct and a sandwich format assay were developed, both based on paper-immobilized quantum dot-oligonucleotide conjugates (Figure 4f, sandwich assay format shown) [92,93]. Hybridization events in both formats brings the QD into close proximity with Cy3 [92].…”

“…Although no RET-based point-of-need applications are fully commercial yet, we see a clear drive towards developing FRET methods to meet the requirements at the PON. However, the inherent requirement of FRET for bulky illumination sources, such as fluorescence microscopes [52,54], lasers [53,104], arc lamps [85] or LEDs [83], is hindering the development of miniaturized or portable devices. In addition, FRET-based techniques suffer from high background levels in most complex samples, especially those containing autofluorescent molecules.…”

A facile and high-sensitive bio-sensing of the V617F mutation in JAK2 gene by GSH-CdTe-QDs FRET-based sensor

“…Moreover, a FRET experiment for a multicolor paper-based platform has been successfully applied to detect target DNA using green and red-emitting GSH-capped QDs with the limit of detection (LOD) in the range of femtomole [ 16 ]. Furthermore, other research has also been reported for the detection of oligonucleotide targets via FRET principle [ [26] , [27] , [28] , [29] , [30] ]. Till now, no sensor-based approach like GSH-CdTe-QDs probes has been reported for the detection of V617F mutation of JAK2 gene.…”