Paper ionophoresis of carbohydrates. I. Procedures and results for four electrolytes

Abstract

Details are given of procedures for effecting paper ionophoresis of polyhydroxy compounds in the electrolytes borax, sodium arsenite, basic lead acetate, and sodium hydroxide. and for detecting the compounds after ionophoresis. Rates of migration are reported for 96 compounds, including all pentose and hexose sugars, the common disaccharides, all sugar alcohols up to the heptitols, the cyclitols, a number of glycols, and several glycosides and other derivatives of carbohydrates. Some new or improved reagents have been developed for locating carbohydrates on paper strips under various conditions. Sodium arsenite and basic lead acetate are the most effective electrolytes for separating reducing sugars, basic lead acetate is the best for separating sugar alcohols, and borax is the best for simple glycols. Some success has been achieved in correlating the configurations of stereoisomers with their mobilities in paper ionophoresis.

Antigenic Components of the Cell Wall of Streptococcus salivarius

“…described previously (Knox, 1965 (Frahn & Mills, 1959), hexosamines with alkaline acetylacetone and Ehrlich's reagent (Partridge,I 948), and amino acids with ninhydrin (0.2 %) in acetone.…”

“…long, between horizontal glass plates, I -4 cm. thick, in 0.05 Msodium tetraborate (Frahn & Mills, 1959); a potential difference of 800-900 V was applied for 5 hr; cooling was done by doing the experiments in a refrigerator. Carbohydrates were detected with a modified alkaline silver nitrate reagent (Frahn & Mills, 1959).…”

Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues

“…2A). After periodate oxidation, reduction and dephosphorylation (12,14,30) iditol was the only alditol detected (11,14). iditol from the InsP3 was resistant to L-iditol dehydrogenase even under conditions where an internal marker of L-iditol was entirely oxidized (29), and this shows that it was D-iditol (Fig.…”

Cellulase and Xylanase Production by Yeasts of the Genus Trichosporon

“…(Brown, 1970) and (ii) 0.25 mm layers of cellulose with doubledevelopment in butan-I-ol/pyridine/water (6 : 4 : 3, by vol.). Reducing sugars and oligosaccharides were detected by spraying with aniline phosphate and heating for 20 min at I I 5 "C (Frahn & Mills, 1959). Glucose and cello-oligosaccharides appeared as brown spots but their yellow fluorescence under ultraviolet light [Mineralight (UVSL-58) UJ tra-violet Products, California, U.S.A.] provided a more sensitive method of detection.…”