Paracellular intestinal permeability of chickens induced by DON and/or C. jejuni is associated with alterations in tight junction mRNA expression

Buchholz, J. Sophia von; Ruhnau, Daniel; Heß, Claudia; Aschenbach, Jörg R.; Heß, Michael; Awad, W. A.

doi:10.1016/j.micpath.2022.105509

Cited by 11 publications

(8 citation statements)

References 47 publications

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“…It therefore seems that intestinal colonization and extraintestinal spread involve different mechanisms. In addition, the transcript level decrease of JAM2, CLDN5 and CLDN10 at 7 dpi observed for some chicken groups inoculated with both strains in both rooms and having livers contaminated by C. jejuni suggest that the presence of C. jejuni could alter the intestinal epithelial barrier function and enhance the intestinal paracellular permeability, likewise reported in the literature (Lamb-Rosteski et al, 2008;Awad et al, 2020;von Buchholz et al, 2022), leading to extraintestinal spread to internal organs through the bloodstream because of a leaky gut, likely for specific strains only. In view of our results, we suggest that this hypothetical mechanism, leading to the hepatic spread of D2008b, would be enhanced in the presence of G2008b.…”

Section: Discussionsupporting

confidence: 67%

“…After colonizing the gut, the bacterium can disseminate to internal organs such as the liver through the bloodstream (Cox et al, 2005(Cox et al, , 2007Richardson et al, 2011). According to the literature, an increase of the paracellular permeability, associated with alterations of the expression of tight junctions proteins could explain this phenomenon (Lamb-Rosteski et al, 2008;von Buchholz et al, 2022). At the slaughterhouse, sources of contamination are numerous: contaminated feathers, gut leakage caused by the defeathering process, evisceration, and finally cross-contamination via direct contact with various contaminated surfaces within the production environment (Newell et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Observations supporting hypothetical commensalism and competition between two Campylobacter jejuni strains colonizing the broiler chicken gut

et al. 2023

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Campylobacter jejuni is the most prevalent bacterial foodborne pathogen in humans. Given the wide genetic diversity of C. jejuni strains found in poultry production, a better understanding of the relationships between these strains within chickens could lead to better control of this pathogen on farms. In this study, 14-day old broiler chickens were inoculated with two C. jejuni strains (103 or 107 CFU of D2008b and 103 CFU of G2008b, alone or together) that were previously characterized in vitro and that showed an opposite potential to compete for gut colonization in broilers. Liver samples and ileal and cecal contents were collected and used to count total C. jejuni and to quantify the presence of each strain using a strain specific qPCR or PCR approach. Ileal tissue samples were also collected to analyze the relative expression level of tight junction proteins. While a 103 CFU inoculum of D2008b alone was not sufficient to induce intestinal colonization, this strain benefited from the G2008b colonization for its establishment in the gut and its extraintestinal spread. When the inoculum of D2008b was increased to 107 CFU – leading to its intestinal and hepatic colonization – a dominance of G2008b was measured in the gut and D2008b was found earlier in the liver for birds inoculated by both strains. In addition, a transcript level decrease of JAM2, CLDN5 and CLDN10 at 7 dpi and a transcript level increase of ZO1, JAM2, OCLN, CLDN10 were observed at 21 dpi for groups of birds having livers contaminated by C. jejuni. These discoveries suggest that C. jejuni would alter the intestinal barrier function probably to facilitate the hepatic dissemination. By in vitro co-culture assay, a growth arrest of D2008b was observed in the presence of G2008b after 48 h of culture. Based on these results, commensalism and competition seem to occur between both C. jejuni strains, and the dynamics of C. jejuni intestinal colonization and liver spread in broilers appear to be strain dependent. Further in vivo experimentations should be conducted to elucidate the mechanisms of commensalism and competition between strains in order to develop adequate on-farm control strategies.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Observations supporting hypothetical commensalism and competition between two Campylobacter jejuni strains colonizing the broiler chicken gut

et al. 2023

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“…Additionally, each sample was tested for a possible genomic DNA contamination and ran in duplex without reverse transcriptase for each gene of interest. Gene-specific sequences for primers for the genes of interest (CLDN-1, -5, OCLN, ZO-1, MUC-2 and cytokines: IL-1β, IL-10, Il-13, IFN-γ, TLR-4) were derived from sequence information in GeneBank as described ( von Buchholz et al, 2021, 2022 ). Two replicates per sample were analyzed with normalization to suitable reference genes (TBP and RPL-13).…”

Section: Methodsmentioning

confidence: 99%

Comparing effects of natural betaine and betaine hydrochloride on gut physiology in broiler chickens

Awad

Ruhnau

Gavrău³

et al. 2022

Poultry Science

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“…1 ). These tight junctions are a critical element of the gut barrier, maintained at a high energy cost by the epithelial cells ( von Bucholz et al., 2022 ). Changes in the expression level and functioning of tight junctions cause gut leakage, characterized by body fluids leaking into the intestinal lumen, which may ultimately result in wet faeces ( Binienda et al., 2020 ).…”

Section: The Physiology Of Gut Healthmentioning

confidence: 99%

Poultry gut health and beyond

Ducatelle¹,

Goossens²,

Eeckhaut³

et al. 2023

Animal Nutrition

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Paracellular intestinal permeability of chickens induced by DON and/or C. jejuni is associated with alterations in tight junction mRNA expression

Cited by 11 publications

References 47 publications

Observations supporting hypothetical commensalism and competition between two Campylobacter jejuni strains colonizing the broiler chicken gut

Observations supporting hypothetical commensalism and competition between two Campylobacter jejuni strains colonizing the broiler chicken gut

Comparing effects of natural betaine and betaine hydrochloride on gut physiology in broiler chickens

Poultry gut health and beyond

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