Parallel DNA Extraction From Whole Blood for Rapid Sample Generation in Genetic Epidemiological Studies

Lee, Kiara; Tripathi, Anubhav

doi:10.3389/fgene.2020.00374

Cited by 12 publications

(10 citation statements)

References 21 publications

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“…Alternatives to microfluidic SPE have been reported. For example, magnetic beads can be displaced through different reagent chambers and pre-concentrate them in a spot with the use of an external magnet, which simplifies the washing and elution of the DNA [182,187]. In another approach, DEP was used to concentrate cancerrelated cfDNA from blood samples in just 10 min and then extracted for downstream sequencing and PCR analysis [188].…”

Section: Nucleic Acid Biomarkersmentioning

confidence: 99%

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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Biomarkers are relevant indicators of the physiological state of an individual.Although biomarkers can be found in diseased tissue and different biofluids, sampling from blood plasma is relatively easy and less invasive. Among the molecular biomarkers that can be found circulating in plasma are proteins, metabolites, nucleic acids, and exosomes. Some of these plasma-circulating biomarkers are now employed for patient stratification in a broad range of diseases with high sensitivity and specificity and are useful in early diagnosis, initial risk assessment, and therapy selection. However, there is a pressing need to develop novel approaches for biomarker analysis that can be translated into clinical or other settings without complex methodologies or instrumentation. Microfluidics has been touted as a promising technology to carry out this task because it offers high-throughput, automation, multiplexed detection, and portability, possibly overcoming the bottleneck that prevent the translation of novel biomarkers to the point-of-care (POC). Here, we provide a review of the microfluidic systems that have been engineered to detect circulating molecular biomarkers in blood plasma. We also review the different microfluidic approaches for plasma enrichment, which are now being integrated with microfluidic-based biomarker analyzers. Such integration should lead to

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Section: Nucleic Acid Biomarkersmentioning

confidence: 99%

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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“…[238][239][240] More recently, these NP-based extraction approaches have been incorporated into microfluidic chips in order to simplify and automate sample processing. [236,237,[241][242][243] Within the microfluidic methods that utilize NPs, many are aimed at on-chip nucleic acid extraction. [243][244][245] Recently, Lee and Tripathi demonstrated on-chip DNA extraction and purification of DNA from whole blood samples facilitated by paramagnetic NPs that bind to DNA and are cleared from debris by on-chip wash steps (Figure 3C).…”

Section: Nanoparticlesmentioning

confidence: 99%

“…[236,237,[241][242][243] Within the microfluidic methods that utilize NPs, many are aimed at on-chip nucleic acid extraction. [243][244][245] Recently, Lee and Tripathi demonstrated on-chip DNA extraction and purification of DNA from whole blood samples facilitated by paramagnetic NPs that bind to DNA and are cleared from debris by on-chip wash steps (Figure 3C). [243] Extraction and pre-concentration procedures like this are amenable to automation and scale-up which make them promising for biosensors and molecular diagnostics.…”

Section: Nanoparticlesmentioning

confidence: 99%

“…[243][244][245] Recently, Lee and Tripathi demonstrated on-chip DNA extraction and purification of DNA from whole blood samples facilitated by paramagnetic NPs that bind to DNA and are cleared from debris by on-chip wash steps (Figure 3C). [243] Extraction and pre-concentration procedures like this are amenable to automation and scale-up which make them promising for biosensors and molecular diagnostics. [237] Other examples of microfluidic systems that incorporate NPs are aimed toward isolation and characterization of rare target cells from a heterogeneous sample.…”

Section: Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

Welch

Powell

Clevinger

et al. 2021

Adv Funct Materials

115

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Nanoscale materials have unique properties that make them especially useful for biomedical diagnostic applications. Recent developments in nanoengineering have resulted in increasing use of nanostructures in biosensors. Various types of 0D, 1D, 2D, and 3D nanostructures have been used to improve biosensor sensitivity, selectivity, limit of detection, and time to result, among other metrics. These nanostructures have been integrated into electrochemical, optical, and other biosensors for this purpose. Here, the most recent advances in the use of nanostructured materials in biosensors are described. This includes a discussion of nanoparticles, nanorods, nanofibers, nanopillars, nanowires, nanosheets, indented nanopatterns (nanoholes and nanoslits), nanogaps, nanochannels, nanopores, nanofunctionalized surfaces, and complex hierarchical structures and their unique advantages and applications in biosensors. Clinical applications of these nanobiosensors are also highlighted along with a discussion of the future directions for these materials in diagnostics.

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“…After binding, beads are separated from contaminating cellular components, washed, and exposed to ethanol to obtain a purified DNA elution [101]. The majority of LOCs based on a magnetic beads NA extraction utilize beads with silica coatings (Table 1); they are added and mixed to sample and lysis buffers [28,[49][50][51]54,60,61,63,64,67,70,71,73]. After cell lysis, DNA-bound beads are driven and collected by a magnetic coil, while the unbound debris is removed by a stream of washing buffers.…”

Section: Magnetic Particlesmentioning

confidence: 99%

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

Obino

Vassalli

Franceschi

et al. 2021

Sensors

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Nucleic acid (NA) extraction is a basic step for genetic analysis, from scientific research to diagnostic and forensic applications. It aims at preparing samples for its application with biomolecular technologies such as isothermal and non-isothermal amplification, hybridization, electrophoresis, Sanger sequencing and next-generation sequencing. Multiple steps are involved in NA collection from raw samples, including cell separation from the rest of the specimen, cell lysis, NA isolation and release. Typically, this process needs molecular biology facilities, specialized instrumentation and labor-intensive operations. Microfluidic devices have been developed to analyze NA samples with high efficacy and sensitivity. In this context, the integration within the chip of the sample preparation phase is crucial to leverage the promise of portable, fast, user-friendly and economic point-of-care solutions. This review presents an overview of existing lab-on-a-chip (LOC) solutions designed to provide automated NA extraction from human raw biological fluids, such as whole blood, excreta (urine and feces), saliva. It mainly focuses on LOC implementation aspects, aiming to describe a detailed panorama of strategies implemented for different human raw sample preparations.

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Parallel DNA Extraction From Whole Blood for Rapid Sample Generation in Genetic Epidemiological Studies

Cited by 12 publications

References 21 publications

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

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