Parameters in fractional laser assisted delivery of topical anesthetics: Role of laser type and laser settings

Meesters, Arne A.; Nieboer, Marilin J.; Kežić, Sanja; Rie, Menno A. de; Wolkerstorfer, Albert

doi:10.1002/lsm.22936

Cited by 16 publications

(9 citation statements)

References 19 publications

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“…On the other hand, the uptake of ingenol mebutate, a hydrophobic drug (LogP 2.49), was shown to increase only 1.6-3.9 fold in dermis after 21 hours depending on laser density but independent of laser pulse energy [15]. Similar results have been reported for diclofenac (LogP 4.98) with 1.4-2.9 fold increase in skin uptake after 24 hours depending on laser density [16] and in vivo for articaine/ epinephrine (LogP 1.9/À0.82) [17], thus indicating that an increase in density might increase the topical uptake of vismodegib. For 5-fluorouracil and ingenol mebutate the effect of AFL exposure was more pronounced in deeper skin layers.…”

Section: Discussionsupporting

confidence: 79%

Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

et al. 2018

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Section: Discussionsupporting

confidence: 79%

Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

et al. 2018

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“…Ablative lasers have the capability to remove or compromise the stratum corneum through their photothermal effect, thus promoting transdermal delivery of drugs . Fractional Er:YAG laser (2,940 nm) is a common type of ablative laser that can form a series of microscopic vertical channels of ablated tissue on the skin surface.…”

Section: Introductionmentioning

confidence: 99%

Transdermal delivery of water‐soluble fluorescent antibody mediated by fractional Er:YAG laser for the diagnosis of lupus erythematosus in mice

et al. 2018

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Objectives Although transdermal drug delivery system (TDDS) has been successfully used for delivering small molecules, its application in the delivery of diagnostic antibodies has been limited due to their large size. In this study, we aim to obtain a broad insight in the dynamics of TRITC‐conjugated Goat Anti‐Mouse IgG (T‐IgG) uptake in fractional Er:YAG laser pretreated skin and provide a new technical option for detecting lupus erythematosus (LE) in mice. Methods The skins of SD and MRL/lpr mice were treated by fractional Er:YAG laser followed by external application of T‐IgG. The classic Franz diffusion method was used to observe the effects of different fractional fluences, densities and antibody concentrations on transdermal delivery of T‐IgG at different time points (2, 4, 6, 8, 20, and 24 hours). Frozen tissue sections and confocal microscopy were used to observe the distribution of T‐IgG on the sagittal and coronal planes of murine skin. Results Increased laser fluence (12.5 J/cm2 to 37.5 J/cm2) within 24 hours resulted in the obvious increase in transdermal amounts of T‐IgG during the early stage (before 8 hours). However, increasing laser density (100 pores/cm2 to 200 pores/cm2) produced a significant increase in T‐IgG permeation during the late stage (20 and 24 hours). Unlike fluence and density, increase in T‐IgG loading concentration (0.5 to 2 μg/μl) led to continuous increase in the whole process of transdermal delivery. T‐IgG appeared in the micro‐pores of SD mice skin within 4 hours after treatment in vivo. After 24 hours, it was observed in the skin. In MRL/lpr mice, positive lupus band testing (LBT) could be found on the skin lesion after laser and T‐IgG external application. Conclusions Fractional Er:YAG laser can help antibodies (150 kDa) to implement effective and controllable transdermal delivery. LBT can be achieved in MRL/lpr mice using TDDS in vivo, which may contribute to the minimally invasive diagnosis of LE. Lasers Surg. Med. 51:268–277, 2019. © 2018 Wiley Periodicals, Inc.

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“…This lateral diffusion was greater than that of the CO 2 laser-induced microchannels and could be due to the establishment of thermal coagulation around the MTZ by the CO 2 laser. The thick coagulation restricted permeant passage across the microchannels [37]. The fully ablative Er:YAG laser at 2940 nm was effective for controlled peeling of the SC with minimal thermal effect according to the histological examination.…”

Section: Discussionmentioning

confidence: 96%

Cutaneous Delivery of Cosmeceutical Peptides Enhanced by Picosecond- and Nanosecond-Domain Nd:YAG Lasers with Quick Recovery of the Skin Barrier Function: Comparison with Microsecond-Domain Ablative Lasers

et al. 2022

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Picosecond or nanosecond-domain non-ablative lasers generate faster photothermal effects and cause less injury than microsecond lasers. In this study, we investigated the enhancing effect of 1064 nm picosecond- and nanosecond-domain neodymium (Nd):yttrium–aluminum–garnet (YAG) lasers on the cutaneous delivery of cosmeceutical peptides. Microsecond-domain fractional ablative CO2 and fully ablative erbium (Er):YAG lasers were also used for comparison. In the Franz diffusion cell study, pig or mouse skin was treated with a laser before exposure to palmitoyl tripeptide (PT)-1, PT-38, and copper tripeptide (CT)-1 at a concentration of 150 μM. Psoriasiform, atopic dermatitis (AD)-like, and photoaged skins were also developed as permeation barriers. The non-ablative laser elicited the ultrastructural disruption of the stratum corneum and epidermal vacuolation. All laser modalities significantly increased the skin permeation of peptides in vitro. The non-ablative laser chiefly enhanced peptide delivery to the receptor compartment, whereas the ablative laser mainly increased the intracutaneous peptide deposition. The picosecond- and nanosecond-domain Nd:YAG lasers elevated the amount of PT-1 in the receptor up to 40- and 22-fold compared with untreated skin, respectively. Laser treatment promoted peptide delivery in barrier-deficient and inflamed skins, although this enhancement effect was less than that observed in healthy skin. Fluorescence microscopy indicated the capability of the non-ablative laser to deliver peptides to deeper skin strata. The ablative laser confined the peptide distribution in the epidermis. Confocal microscopy showed that peptides penetrated the skin along the microdots created by the fractional Nd:YAG and CO2 lasers. The skin barrier function determined by transepidermal water loss suggested quick recovery when using a nanosecond-domain laser (within 4 h). A longer period was needed for the skin treated with the fully ablative Er:YAG laser (76–84 h). Nanosecond non-ablative laser-facilitated peptide delivery may become an efficient and safe approach for cosmeceutical applications.

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Parameters in fractional laser assisted delivery of topical anesthetics: Role of laser type and laser settings

Cited by 16 publications

References 19 publications

Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

Topical delivery of vismodegib using ablative fractional laser and micro‐emulsion formulation in vitro

Transdermal delivery of water‐soluble fluorescent antibody mediated by fractional Er:YAG laser for the diagnosis of lupus erythematosus in mice

Cutaneous Delivery of Cosmeceutical Peptides Enhanced by Picosecond- and Nanosecond-Domain Nd:YAG Lasers with Quick Recovery of the Skin Barrier Function: Comparison with Microsecond-Domain Ablative Lasers

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