Parameters influencing the antigen release from spray-dried poly(dl-lactide) microparticles

Development of microparticles prepared by spray-drying as a vaccine delivery system against brucellosis

“…Previously, we published the results obtained with HS microencapsulated in PEC (Murillo et al, 2001a) by the solvent evaporation technique, obtaining that in the mice model of infection was significantly effective against B. ovis and B. abortus (Murillo et al, 2001b). We report here the microencapsulation of HS-antigens with different polymers by the spray-drying technique, recently described by several authors as being appropriate for the encapsulation of antigens for vaccinal purposes (Baras et al, 2000a;Baras et al, 2000b;Baras et al, 2000c Encapsulation efficiencies (EE) were determined after hydrolysis of the samples with NaOH 0.1 N and the amount of antigen was determined by BCA assay at 562 nm (Smith et al, 1985). Release studies after incubation of the particles in PBS at 37 ºC was determined by BCA assay, and the pH of the samples were measured during a the release studies, without changing the medium.…”

Novel bioactive hydrophobic gentamicin carriers for the treatment of intracellular bacterial infections

“…Previously, we published the results obtained with HS microencapsulated in PEC (Murillo et al, 2001a) by the solvent evaporation technique, obtaining that in the mice model of infection was significantly effective against B. ovis and B. abortus (Murillo et al, 2001b). We report here the microencapsulation of HS-antigens with different polymers by the spray-drying technique, recently described by several authors as being appropriate for the encapsulation of antigens for vaccinal purposes (Baras et al, 2000a;Baras et al, 2000b;Baras et al, 2000c).…”

Preparation of dextran–poly(lactide)–1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine copolymer and its micellar characteristics

“…PLA is a kind of biodegradable materials with low toxicity, excellent biocompatibility and bioabsorbability in vivo (Baras, Benoit, & Gillard, 2000;Perez et al, 2001). Certain polyethylene glycol/phosphatidylethanolamine (PEG-PE) conjugates, as DPPE, may form very stable micelle in aqueous medium (Gao, Lukyanov, Anurag Singhal, Vladimir, & Torchilin, 2002;Lukyanov, Elbayoumi, Chakilam, & Torchilin, 2004).…”