Parasite detection efficiencies of five stool concentration systems

Abstract

Fresh fecal material that was free of ova and parasites was pooled with 10% Formalin in a 1:4 ratio to prepare a standard specimen. Portions of 100 ml of this specimen were individually seeded with Cryptosporidium oocysts, Entamoeba coli, Entamoeba histolytica, and Giardia lamblia cysts; ova of Necator americanus; and Strongyloides larvae. Appropriate volumes of each parasite suspension were used to evaluate the Fecal Concentrator Kit (Remel, Lenexa, Kans.

Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis

“…For the last thirty years, fecal concentration tubes were usually used, over this period of time various modifications have been made to the system improving the yield of eggs, cysts, and trophozoites of different parasites of variable sizes and shapes 27 . Closed fecal concentration tubes give an effective satisfactory diagnosis of different intestinal parasites 28 . Concentration methods are modified by using a small size sieve (425 μm) in the Parasep O&P filter fecal concentrator tubes which trap rejected particles and debris in stool samples, preventing their extrusion into the sedimentation cone during centrifugation.…”

Helminth prevalence among adults in rural Kenya: a stool survey for soil-transmitted helminths and schistosomiasis in Nyanza province

“…The earlier generation of this concentration method (Para-Pak Macro-Con), has performed similarly to four other concentration techniques: Fecal Concentrator Kit (Remel, Lenexa, KS, USA), Fecal Parasite Concentrator (Evergreen Scientific, Los Angeles, CA, USA), Trend FeKal CON-Trate (Trend Scientific, Inc., St. Paul, MN, USA) and a standardized gauze filtration method. 10 Since stools were examined when they were fresh, the standard merthiolate-iodine formaldehyde stain was not required since both merthiolate and formaldehyde are preservatives. Rather, specimens stained with Dobell's iodine were visualized under 40 x magnification light microscopy.…”

Incidence and characteristics of endemic Norwalk‐like virus‐associated gastroenteritis

“…Pathogenic Escherichia coli were characterised as previously described [Robins-Browne et al, 1993;Paton and Paton, 1998]. A permanent stain (modified iron-haematoxylin) was then used on concentrated specimens to detect parasites including Giardia, Cryptosporidium, Blastocystis, and Entamoeba [Zierdt, 1984;Perry et al, 1990].…”