Parasitism ofTrichodermaspp. onRhizoctonia solaniandSclerotium rolfsii-- Scanning Electron Microscopy and Fluorescence Microscopy

Elad, Y.; Chet, I.; Boyle, P; Henis, Y.

doi:10.1094/phyto-73-85

Cited by 273 publications

(118 citation statements)

References 17 publications

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“…(Toppo and Naik, 2015). Fungi like Trichoderma, and bacteria like Bacillus, Serratia, Alteromonos were reported to have chitinolytic activity (Elad et al, 1982 andTsujibo et al, 1998).…”

Section: Fig 2: Isolated Potent Bacteria From Marine Sediment Samplementioning

confidence: 99%

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana¹,

Sasikala²

2017

Int. J. Adv. Res. Biol. Sci.

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In the present work marine sediment samples were collected from Thengapattanam estuary on the south west coast of India for the isolation and screening of bacteria for biocidal potency. The density of the bacterial load in the sediment samples was ranged from 5.43x10 4 to 2.8x10 6 CFU/g. Screening of bacterial isolates for antagonistic activity revealed that the isolate MS21 had the highest zone of clearance among against all the 10 plant fungal pathogens tested. Among 10 fungal pathogens, the highest antagonistic activity was observed against Gleosporium gleosporioide (36mm) and the lowest against Colletotrichum lamella (11mm). The potential isolate was identified as Bacillus subtilis using biochemical methods and it was designated as Bacillus subtilis MS21. Effect of various physicochemical parameters on growth of B. subtilis MS21 showed that 36 hrs of incubation period, 150 rpm agitation, pH-8.0, temperature-35 o C, salinity-1.0%, 2% sucrose as carbon source and 1% beef extract as nitrogen source were found to be the ideal conditions for maximum growth. Maximum growth of 1.92 OD was observed in mass scale culture with the optimized ideal conditions in the shake flask.

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“…(Toppo and Naik, 2015). Fungi like Trichoderma, and bacteria like Bacillus, Serratia, Alteromonos were reported to have chitinolytic activity (Elad et al, 1982 andTsujibo et al, 1998).…”

Section: Fig 2: Isolated Potent Bacteria From Marine Sediment Samplementioning

confidence: 99%

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana¹,

Sasikala²

2017

Int. J. Adv. Res. Biol. Sci.

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“…Most of these studies deal with Trichoderma species (Elad et al, 1983;Donzelli and Harman, 2001). In particular, the application of Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Mycophagous growth of Collimonas bacteria in natural soils, impact on fungal biomass turnover and interactions with mycophagous Trichoderma fungi

et al. 2008

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Bacteria of the genus Collimonas are widely distributed in soils, although at low densities. In the laboratory, they were shown to be mycophagous, that is, they are able to grow at the expense of living hyphae. However, so far the importance of mycophagy for growth and survival of collimonads in natural soil habitats is unknown. Using a Collimonas-specific real-time PCR assay, we show here that the invasion of field soils by fungal hyphae (Absidia sp.) resulted in a short-term, significant increase (average fourfold) of indigenous collimonads. No such responses were observed for other soil bacteria studied (Pseudomonas, Burkholderia, PCR-denaturing gradient gel electrophoresis patterns of total bacteria and Burkholderia). Hence, it appears that the stimulation of growth of Collimonas bacteria by fungal hyphae is not common among other soil bacteria. In the same field soils, Trichoderma, a fungal genus known for mycophagous (mycoparasitic) growth, increased upon introduction of Absidia hyphae. Hence, mycophagous growth by Collimonas and Trichoderma can occur in the same soils. However, in controlled experiments (sand microcosms), collimonads appeared to have a negative effect on mycophagous growth of a Trichoderma strain. The effect of mycophagous growth of collimonads on fungal biomass dynamics was studied in sand microcosms using the same Absidia sp. as a test fungus. The growth of collimonads did not cause a significant reduction in the Absidia biomass. Overall, the study indicates that mycophagous nutrition may be important for collimonads in natural soils, but the impact on fungal biomass turnover is likely to be minor.

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“…and pathogenic fungi appears to be the release of lytic enzymes, including chitinases. C hitinases are produced by Trichoderma harzianum when it is grown in the presence of chitin or isolated fungal cell walls (Elad et al, 1983;Ridout et al, 1988;Tokimoto, 1982); however, other nutritional conditions that promote synthesis of this enzyme have not been investigated. Furthermore, it is not known whether the induction of chitinase by chitin in T. harzianum is effected by the same inducer-repressor mechanism as has been proposed for other hydrolases produced by Trichoderma strains (Merivuori et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Regulation of chitinase synthesis in Trichoderma harzianum

Ulhôa

Peberdy

1991

Journal of General Microbiology

102

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The production of chitinase by Trichoderma species is of interest in relation to their use in biocontrol and as a source of mycolytic enzymes. Fourteen isolates of the genus were screened to identify the most effective producer of chitinase. The best strain for chitinase was Trichoderma hurziunum 39.1, and this was selected for study of the regulation of enzyme synthesis. Washed mycelium of T. hurziunum 39.1 was incubated with a range of carbon sources. Chitinase synthesis was induced on chitin-containing medium, but repressed by glucose and Nacetylglucosamine. Production of the enzyme was optimal at a chitin concentration of 0.5 %, at 28 "C, pH 6.0 and was independent of the age of the mycelium. The synthesis of chitinase was blocked by both 8-hydroxyquinoline and cycloheximide, inhibitors of RNA and protein synthesis, respectively. The mode of chitinase synthesis in this fungus is discussed.

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Parasitism ofTrichodermaspp. onRhizoctonia solaniandSclerotium rolfsii-- Scanning Electron Microscopy and Fluorescence Microscopy

Cited by 273 publications

References 17 publications

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Mycophagous growth of Collimonas bacteria in natural soils, impact on fungal biomass turnover and interactions with mycophagous Trichoderma fungi

Regulation of chitinase synthesis in Trichoderma harzianum

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