Parasola malakandensis sp. nov. (Psathyrellaceae; Basidiomycota) from Malakand, Pakistan

Hussain, Shah; Afshan, Najam‐ul‐Sehar; Ahmad, Hassan; Khalid, Abdul Nasir; Niazi, Abdul Rehman

doi:10.1016/j.myc.2016.09.002

Cited by 14 publications

(13 citation statements)

References 15 publications

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“…We amplified nuc rDNA internal transcribed spacer (ITS) and 28S loci and translation elongation factor 1α gene ( TEF1α ) using the primer combinations ITS1F/ITS4; LR0R/LR5 and EF1-983F/EF1-1567R, respectively (White et al 1990, Gardes and Bruns 1993, Rehner and Buckley 2005). For PCR amplification, we followed Hussain et al (2017). PCR products were purified using the QIAquick PCR Purification kit (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

“…Redhead, Vilgalys & Hopple, P. lilatincta (Bender & Uljé) Redhead, Vilgalys & Hopple, P. malakandensis S. Hussain, N. Afshan & H. Ahmad, P. plicatilis and P. setulosa (Berk. & Broome) Redhead, Vilgalys & Hopple) have been reported from Pakistan (Ahmad 1980, Hussain et al 2016, 2017). In this study, we describe two new species P. glabra and P. pseudolactea , based on morphological characters and phylogenetic analyses of nuc rDNA region encompassing the internal transcribed spacers 1 and 2, along with the 5.8S rDNA (ITS), nuc 28S rDNA D1-D2 domains (28S) and translation elongation factor 1α gene ( TEF1α ).…”

Section: Introductionmentioning

confidence: 97%

“…The genus is well documented in Europe (Orton and Watling 1979, Uljé and Bas 1988, Uljé and Bender 1997, Schafer 2014, Szarkándi et al in press). Some species are reported from North America, Africa, Lesser Antilles (Pegler 1966, 1983, Dennis 1970), and Asia (Ahmad 1980, Pegler 1986, Hongo 1987, Hussain et al 2016, 2017) and Australia (Grgurinovic 1997). …”

Section: Introductionmentioning

confidence: 99%

“…Basidiospore shape and size are the main descriptive features for species identification in Parasola (Nagy et al 2009, 2010, Schafer 2014, Hussain et al 2017). …”

Section: Introductionmentioning

confidence: 99%

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The genus Parasola in Pakistan with the description of two new species

Hussain¹,

Ahmad²,

Ullah³

et al. 2018

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Parasola is a genus of small, veil-less coprinoid mushrooms in the family Psathyrellaceae (Agaricales). The genus is not well documented in Asia, specifically in Pakistan. In this study we describe two new species Parasola glabra and P. pseudolactea from Pakistan, based on morphological and molecular data. Phylogeny based on three DNA regions: nuc rDNA region encompassing the internal transcribed spacers 1 and 2 along with the 5.8S rDNA (ITS), nuc 28S rDNA D1-D2 domains (28S) and translation elongation factor 1α gene (TEF1α) show that the new taxa are clustered in a clade formed by the members of section Parasola of genus Parasola. Parasola glabra with grayish pileus, slightly depressed pileal disc, lamellae separated from the stipe by pseudocollarium, basidiospores 14.5–16.5 × 9.5–11.5 × 8.0–10.5 µm, in front view broadly ovoid to oblong, some with rhomboidal outline, in side view ellipsoid, with eccentric germ-pore of 1.5 µm diameter. Parasola pseudolactea with yellowish brown to dull brown pileus, disc indistinctly umbonate, lamellae free, pseudocollarium absent, basidiospores 13.5–14.5 × 10.5–12.0 × 9.5–10.5 µm, in face view rounded triangular to heart shaped, rarely ovoid to subglobose, in side view ellipsoid to oblong, with eccentric germ-pore of 1.5 µm diam. In addition to these new species, P. auricoma and P. lilatincta were also studied. Morphological descriptions for the new species and comparison with known Parasola species are provided. Our observations highlight the diversity of Parasola in northern Pakistan and further document the need for additional systematic focus on the region’s fungi.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

“…Basidiospore shape and size are the main descriptive features for species identification in Parasola (Nagy et al 2009, 2010, Schafer 2014, Hussain et al 2017). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The genus Parasola in Pakistan with the description of two new species

Hussain¹,

Ahmad²,

Ullah³

et al. 2018

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“…Western Himalaya, part of Himalaya in Pakistan, is also floristically diversified with more than 750 plant species and more than 300 macrofungi (Ahmad et al, 1997;Samina and Salman, 2012;Razaq et al, 2016;Hussain et al, 2017). Mostly these Himalayan areas are distinguished by the luxurious growth of coniferous mixed with deciduous vegetation and eye catching mushrooms especially during rainy season.…”

Section: Introductionmentioning

confidence: 99%

Phylogeny and Taxonomy of Hebeloma theobrominum and H. mesophaeum from Western Himalaya

Razaq¹,

Ilyas²,

Khalid³

2017

IJAB

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This study deals with the molecular systematics of Asian collections of Hebeloma theobrominum and H. mesophaeum which have not been previously reported from any Himalayan part of the world, and are new to Pakistan. Both were identified using morpho-anatomical and molecular characterization. The target rDNA of both species was amplified by using universal fungal primers. In phylogenetic analysis, both species distinctly clustered with their respective groups. These both species have been described first time from Asia using ITS-rDNA barcoding. The sequences of both species were compared with those of same European species from the GenBank. These species clustered with European species in phylogenetic tree proving their cosmopolitan distribution.

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