Parental competition for the regulators of chromatin dynamics in mouse zygotes

Ooga, Masatoshi; Inoue, Rei; Kazama, Kousuke; Wakayama, Sayaka; Kamimura, Satoshi; Wakayama, Teruhiko

doi:10.1038/s42003-022-03623-2

Cited by 4 publications

(4 citation statements)

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“…4 ). Indeed, the H3.3 level in rs-mPN was significantly lower than that in sp-mPN [ 54 ], which is consistent with our findings because the retained histone H3 from round spermatids presumably prohibited zygotic H3 deposition. Thus, in addition to oogenesis, spermiogenesis is important for chromatin structure and associated gene expression in early preimplantation embryos.…”

Section: Paternal Contribution To the Zygotic Chromatin Structuresupporting

confidence: 90%

“…To examine the mechanisms underlying asymmetric parental histone mobility, several types of zygotes prepared using a micromanipulator were examined [ 54 ]. We hypothesized that protamine-histone replacement is important for the acquisition of high histone mobility in male PN.…”

Section: Chromatin Looseness and Accessibility In Zygotes And Preimpl...mentioning

confidence: 99%

“…Sp-mPN has higher transcriptional activity than female PN [ 57 ], presumably because of its looser chromatin structure compared to female PN. Therefore, we examined transcriptional activity in delayed ICSI zygotes and found that the parental disrupted asymmetric transcriptional activity [ 54 , 57 ]. Thus, parental asymmetric chromatin looseness is involved in parental asymmetric transcriptional activity.…”

Section: Chromatin Looseness and Accessibility In Zygotes And Preimpl...mentioning

confidence: 99%

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Chromatin structure in totipotent mouse early preimplantation embryos

OOGA

2024

Journal of Reproduction and Development

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Totipotency refers to the ability of a single cell to give rise to all the different cell types in the body. Terminally differentiated germ cells (sperm and oocytes) undergo reprogramming, which results in the acquisition of totipotency in zygotes. Since the 1990s, numerous studies have focused on the mechanisms of totipotency. With the emergence of the concept of epigenetic reprogramming, which is important for the undifferentiated and differentiated states of cells, the epigenomes of germ cells and fertilized oocytes have been thoroughly analyzed. However, in early immunostaining studies, detailed epigenomic information was difficult to obtain. In recent years, the explosive development of next-generation sequencing has made it possible to acquire genome-wide information and the rise of genome editing has facilitated the analysis of knockout mice, which was previously difficult. In addition, live imaging can effectively analyze zygotes and 2-cell embryos, for which the number of samples is limited, and provides biological insights that cannot be obtained by other methods. In this review, the progress of our research using these advanced techniques is traced back from the present to its earliest years.

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Section: Paternal Contribution To the Zygotic Chromatin Structuresupporting

confidence: 90%

Section: Chromatin Looseness and Accessibility In Zygotes And Preimpl...mentioning

confidence: 99%

Section: Chromatin Looseness and Accessibility In Zygotes And Preimpl...mentioning

confidence: 99%

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Chromatin structure in totipotent mouse early preimplantation embryos

OOGA

2024

Journal of Reproduction and Development

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“…A 5-EU assay of the embryos was performed as described previously ( 32 ), with slight modifications. Briefly, the embryos were transferred to KSOM medium containing 2 mM 5-EU (C10330, Thermo Fisher Scientific) and cultured for 2 h. Then the embryos were fixed with 3.7% PFA for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

H3.1/3.2 regulate the initial progression of the gene expression program

Funaya,

Takahashi,

Suzuki

et al. 2024

Nucleic Acids Research

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In mice, transcription from the zygotic genome is initiated at the mid-one-cell stage, and occurs promiscuously in many areas of the genome, including intergenic regions. Regulated transcription from selected genes is established during the two-cell stage. This dramatic change in the gene expression pattern marks the initiation of the gene expression program and is essential for early development. We investigated the involvement of the histone variants H3.1/3.2 in the regulation of changes in gene expression pattern during the two-cell stage. Immunocytochemistry analysis showed low nuclear deposition of H3.1/3.2 in the one-cell stage, followed by a rapid increase in the late two-cell stage. Where chromatin structure is normally closed between the one- and two-cell stages, it remained open until the late two-cell stage when H3.1/3.2 were knocked down by small interfering RNA. Hi-C analysis showed that the formation of the topologically associating domain was disrupted in H3.1/3.2 knockdown (KD) embryos. Promiscuous transcription was also maintained in the late two-cell stage in H3.1/3.2 KD embryos. These results demonstrate that H3.1/3.2 are involved in the initial process of the gene expression program after fertilization, through the formation of a closed chromatin structure to execute regulated gene expression during the two-cell stage.

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