Partial rapid metabolism of 1,2‐dichloroethane by <i>Methylosinus trichosporium</i> OB‐3b

Batch culture conditions were established for the formation of H(2)-driven whole-cell soluble or particulate methane monooxygenase (sMMO or pMMO) activity in the obligate methanotroph, Methylosinus trichosporum Ob3b, to expand its potential uses in groundwater bioremediation and the production of specific chemicals. Addition of either Ni and H(2) to a nitrate-containing minimal salts growth medium or Ni and Mo to a nitrate-lacking growth medium (induces a nitrogenase that generates intracellular H(2)) markedly enhanced both the hydrogenase and the accompanying washed-cell H(2)-driven MMO activities of shake-flask cultured cells. For sMMO containing cells, H(2) provided in vitro reducing power for the oxidation of chlorinated solvents such as chloroform and trichloroethylene. Cell cultivations under N(2)-fixing conditions in a 5-L bioreactor, however, required an initial nitrate concentration of at least 1 to 2 mM to achieve high biomass yields (5 to 7 g of dry cell wt/L) for cells producing H(2)-driven sMMO or pMMO activity. Elevation of the initial medium nitrate concentration to 20 mM shortened the culture time for pMMO producing cells by 40%, yet still generated an equivalent growth yield. High nitrate also shortened the culture time for sMMO containing cells by approximately 25%, but it lowered their biomass yield by 26%. Upon storage for 5 weeks at room temperature, washed resting-state cells retained 90% and 70% of their H(2)-driven sMMO and pMMO activity, respectively. This makes their practical use quite feasible. (c) 1995 John Wiley & Sons, Inc.

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Biodehalogenation: The metabolism of vinyl chloride by Methylosinus trichosporium OB‐3b. A sequential oxidative and reductive pathway through chloroethylene oxide

Castro

Riebeth

Belser

1992

Enviro Toxic and Chemistry

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Resting cell suspensions of the soil methylotroph Methylosinus trichosporium OB‐3b rapidly metabolize vinyl chloride The half‐life of the reaction, based on Cl release at pH 7 4, 0 1 in phosphate buffer with a cell density of 0 1 g/ml, is 0 61 h The path of metabolism is complicated but proceeds through the intermediacy of chloroethylene oxide Independent studies of the hydrolysis of this oxirane in water set its chemical half life at 3 9 min The products of its reaction with water are chloroacetaldehyde and hydroxyacetaldehyde They are produced in a ratio of 2 1, as judged by Cl release and 1H and 13C nuclear magnetic resonance analysis Incubations with vinyl chloride 1,2‐13C and vinyl chloride 1,2 14C establish that the dominant metabolic pathway entails a quick reduction of chloroethylene oxide to ethylene oxide Ethylene oxide is then converted in sequence to ethylene glycol, hydroxyacetaldehyde, glycolic acid, and CO2 This process is competitive with the chemical hydrolysis of chloroethylene oxide Similarly, chloroacetaldehyde is con verted to chloroacetic acid The latter is only slowly metabolized by this organism, and it accumulates throughout incubation to a final yield of 10% It is concluded that the organism participates in the hydrolysis of both chloroethylene oxide and ethylene oxide

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Partial rapid metabolism of 1,2‐dichloroethane by Methylosinus trichosporium OB‐3b

Cited by 13 publications

References 21 publications

Batch cultivation ofMethylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions

Batch cultivation ofMethylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions

Batch cultivation of Methylosinus trichosporium OB3B: IV. Production of hydrogen‐driven soluble or particulate methane monooxygenase activity

Biodehalogenation: The metabolism of vinyl chloride by Methylosinus trichosporium OB‐3b. A sequential oxidative and reductive pathway through chloroethylene oxide

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