Parvovirus B19 seroconversion in a cohort of human immunodeficiency virus-infected patients

Azevedo, Kátia Martins Lopes de; Setúbal, Sérgio; Camacho, Luiz Antônio Bastos; Garcia, Rita de Cássia Nasser Cubel; Siqueira, Marilda Mendonça; Pereira, Renata Freire Alves; Oliveira, Solange Artimos de

doi:10.1590/s0074-02762012000300010

Cited by 9 publications

(11 citation statements)

References 31 publications

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“…Data, such as haemoglobin (Hb) concentration, CD4 + T cell counts and B19V IgG and IgM serology, were retrieved from a previous study by our group (de Azevedo et al 2012). ELISAs (Biotrin International TM , Dublin, Ireland) were performed, according to the manufacturer’s instructions, to detect IgG and IgM antibodies to B19 in all sera.…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

“…Briefly, viral DNA was extracted from serum samples of 88 HIV-positive patients using a QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. For the screening of B19V DNA, PCR was performed using the primers E1905F (nt 1905-1923) and E1987R (nt 2007-1987), which amplify a 102-bp fragment of the NS1 gene as previously described by Nguyen et al (2002), with some modifications (de Azevedo et al 2012). This PCR can routinely detect as few as 20 copies of the B19V DNA (Nguyen et al 2012).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

“…In human immunodeficiency virus (HIV)-infected patients with residual immunity, the clinical manifestations, if any, are those of fifth disease (Frickhofen & Young 1990). With the advent of highly active antiretroviral treatment (HAART), several studies, including one from our group (de Azevedo et al 2012), have shown a decrease in cases of anaemia caused by B19V (Mylonakis et al 1999, Ware & Moore 2001). …”

mentioning

confidence: 99%

“…Seroconversions were detected in approximately 30% (28 of 88) of our anti-B19 IgG-negative patients, a similar proportion to that found by others (Chernak et al 1995, Mylonakis et al 1999). Most seroconversions occurred during incidence peaks of a B19V infection in Niterói (Oliveira et al 2005, de Azevedo et al 2012). All sera from the 88 patients were tested by polymerase chain reactions (PCRs) and B19 DNA was detected in five of the patients, four of whom also exhibited seroconversion.…”

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confidence: 99%

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Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

Pereira

Garcia

Azevedo

et al. 2014

Mem. Inst. Oswaldo Cruz

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Immunocompromised patients may develop severe chronic anaemia when infected by human parvovirus B19 (B19V). However, this is not the case in human immunodeficiency virus (HIV)-infected patients with good adherence to highly active antiretroviral treatment (HAART). In this study, we investigated the clinical evolution of five HIV-infected patients receiving HAART who had B19V infections confirmed by serum polymerase chain reaction. Four of the patients were infected with genotype 1a strains and the remaining patient was infected with a genotype 3b strain. Anaemia was detected in three of the patients, but all patients recovered without requiring immunoglobulin and/or blood transfusions. In all cases, the attending physicians did not suspect the B19V infections. There was no apparent relationship between the infecting genotype and the clinical course. In the HAART era, B19V infections in HIV-positive patients may be limited, subtle or unapparent.

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Section: Patients Materials and Methodsmentioning

confidence: 99%

Section: Patients Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

Pereira

Garcia

Azevedo

et al. 2014

Mem. Inst. Oswaldo Cruz

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“…The lowest hb was found in the group on zidovudine therapy (3.5 g/dL) followed by those with PV and/or PRCA (3.6 g/dL). A pancytopenia was present in 11.2% A recent South African study showed that severe anemia is inversely related to cART exposure 10 while other studies have shown that cART improves anemia 8,11 and that increase in CD4 count correlates with improvement of anemia. 12 In this study however, almost one third of patients were virally suppressed and 12.6% of patients had CD4 counts of >350 cells/µL.…”

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confidence: 95%

The multifactorial pathogenesis of severe central anemia in a cohort of HIV‐positive patients

Glatt

Schapkaitz

Vaughan

2019

Int J Lab Hematology

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Frequency and genotype of human parvovirus B19 among Iranian patients infected with HIV

et al. 2015

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The human parvovirus B19 (B19) usually causes a subclinical infection in immunocompetent individuals. Whereas immunocompromised individuals such as patients infected with HIV are at risk of persistent anemia due to B19 infection. Only few studies have been carried out on distribution and molecular epidemiology of B19 in Iran. We aimed to determine the frequency and genotype of B19 among Iranian patients infected with HIV. We conducted a survey on 99 HIV patients and 64 healthy controls. IgG and IgM antibodies against B19 were detected by ELISA and B19 DNA was assessed by nested PCR. PCR products were subjected to direct sequencing and classified after phylogenetic analysis. The prevalence of B19 immunoglobulin was 11.1% for IgG and 1% for IgM. B19 DNA was detected in 13.1% of cases. The prevalence of B19 IgG, IgM, and DNA in control group was 25%, 1.6%, and 9.4%, respectively. B19 IgG was significantly lower in HIV group than in normal controls. There was no significant difference regarding anemia between cases and controls. All sequenced B19 isolates belonged to genotype 1A with low genetic diversity. Our findings indicated that in the HAART era, the importance of B19 infections in HIV patients may be limited whereas persistent B19 viremia in the circulation of healthy controls raises a potential concern in blood donations.

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Parvovirus B19 seroconversion in a cohort of human immunodeficiency virus-infected patients

Cited by 9 publications

References 31 publications

Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

Clinical features and laboratory findings of human parvovirus B19 in human immunodeficiency virus-infected patients

The multifactorial pathogenesis of severe central anemia in a cohort of HIV‐positive patients

Frequency and genotype of human parvovirus B19 among Iranian patients infected with HIV

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