Passion fruit peel as novel substrate for enhanced β-glucosidases production by Penicillium verruculosum: Potential of the crude extract for biomass hydrolysis

Almeida, Janaína Marques de; Lima, Vanderlei Aparecido de; Giloni-Lima, Patrícia Carla; Knob, Adriana

doi:10.1016/j.biombioe.2014.11.002

Cited by 38 publications

(9 citation statements)

References 42 publications

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“…In all cases, the multiple screening processes identified the same important factors as most influential; temperature (X 3 ) and incubation time (X 4 ) were highly statistically significant ( p -value < 0.001), whereas tryptone (X 6 ) and OD 600nm (X 1 ) were statistically significant ( p -value < 0.05). These results align with previous studies that employed factorial design to enhance β-glucosidases expression [46,47]. Other factors, namely IPTG (X 2 ), yeast extract (X 5 ) and fructose (X 7 ), were not statistically significant ( p -value > 0.05).…”

Section: Resultssupporting

confidence: 90%

The Statistical Optimisation of Recombinant β-glucosidase Production through a Two-Stage, Multi-Model, Design of Experiments Approach

Uhoraningoga¹,

Kinsella²,

Frias³

et al. 2019

Bioengineering

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β-glucosidases are a class of enzyme that are widely distributed in the living world, with examples noted in plants, fungi, animals and bacteria. They offer both hydrolysis and synthesis capacity for a wide range of biotechnological processes. However, the availability of native, or the production of recombinant β-glucosidases, is currently a bottleneck in the widespread industrial application of this enzyme. In this present work, the production of recombinant β-glucosidase from Streptomyces griseus was optimised using a Design of Experiments strategy, comprising a two-stage, multi-model design. Three screening models were comparatively employed: Fractional Factorial, Plackett-Burman and Definitive Screening Design. Four variables (temperature, incubation time, tryptone, and OD600 nm) were experimentally identified as having statistically significant effects on the production of S.griseus recombinant β-glucosidase in E. coli BL21 (DE3). The four most influential variables were subsequently used to optimise recombinant β-glucosidase production, employing Central Composite Design under Response Surface Methodology. Optimal levels were identified as: OD600 nm, 0.55; temperature, 26 °C; incubation time, 12 h; and tryptone, 15 g/L. This yielded a 2.62-fold increase in recombinant β-glucosidase production, in comparison to the pre-optimised process. Affinity chromatography resulted in homogeneous, purified β-glucosidase that was characterised in terms of pH stability, metal ion compatibility and kinetic rates for p-nitrophenyl-β-D-glucopyranoside (pNPG) and cellobiose catalysis.

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Section: Resultssupporting

confidence: 90%

The Statistical Optimisation of Recombinant β-glucosidase Production through a Two-Stage, Multi-Model, Design of Experiments Approach

Uhoraningoga¹,

Kinsella²,

Frias³

et al. 2019

Bioengineering

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“…Fermented palm kernel cake was used in another study as the substrate for mannanase production by growing Aspergillus terreus SUK-1 in a column reactor [78]. Sugar cane pulp, soy bean mea, locust bean gum and peels from plantain, mango, potato and passion fruit have also been reported to be excellent choices of raw material for the production of β-mannosidase and β-glucosidases [69,79,80].…”

Section: Industrial Enzymesmentioning

confidence: 99%

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

Ravindran¹,

Hassan²,

Williams³

et al. 2018

Bioengineering

226

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Agro-industrial waste is highly nutritious in nature and facilitates microbial growth. Most agricultural wastes are lignocellulosic in nature; a large fraction of it is composed of carbohydrates. Agricultural residues can thus be used for the production of various value-added products, such as industrially important enzymes. Agro-industrial wastes, such as sugar cane bagasse, corn cob and rice bran, have been widely investigated via different fermentation strategies for the production of enzymes. Solid-state fermentation holds much potential compared with submerged fermentation methods for the utilization of agro-based wastes for enzyme production. This is because the physical–chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. Recent studies have shown that pretreatment technologies can greatly enhance enzyme yields by several fold. This article gives an overview of how agricultural waste can be productively harnessed as a raw material for fermentation. Furthermore, a detailed analysis of studies conducted in the production of different commercially important enzymes using lignocellulosic food waste has been provided.

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“…Como este resíduo é rico em fibras, em especial a pectina, e pode ser facilmente convertido em farinha, ele se torna um objeto de estudo interessante para o aproveitamento como um substrato alternativo (Seixas et al, 2014). Almeida et al (2015) demonstrou o uso da farinha de casca de maracujá para produção de β-glicosidase por Penicillium verruculosum e demonstrou que, frente à utilização de substratos tradicionais, a farinha de casca de maracujá teve a capacidade de aumentar em 5,7 vezes a atividade enzimática. Mais especificamente, já há estudos demonstrando a versatilidade tecnológica da farinha de casca de maracujá na produção de enzimas pectinolíticas, sendo os resultados obtidos, em relação à quantidade e atividade enzimática, superior a outros substratos alternativos (Damásio et al, 2011;Jaramillo et al, 2015).…”

Section: Substratounclassified

“…As leveduras desempenham um papel fundamental na natureza e nos processos biotecnológicos (Satyanarayana & Kunze, 2009;Kurtzman, Fell & Boekhout, 2011;Almeida et al, 2015). Além de seu papel como decompositores primários, seu metabolismo é aproveitado para a geração de commodities industriais de grande importância econômica, tais como enzimas, prebióticos, probióticos, entre outros (Kavanagh, 2017).…”

Section: Introductionunclassified

Resíduos agroindustriais como insumos promissores para obtenção de bioprodutos por leveduras - uma breve revisão

Takeyama

Kawaguti

Koblitz

et al. 2020

RSD

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Yeasts play a fundamental role in nature, and due to their biochemical and physiological versatility, many of them can have their metabolic routes regulated and optimized for the biosynthesis of specific products. In view of this potentiality, new contributions that dedicate to the study of fermentative processes involving yeasts as protagonists, especially for the production of value-added products from lost vegetables and their residues, show importance. Among these products, particularly in this brief review, the production of pectinase and yeast probiotic potential in alternative culture media is mentioned as a way to make these processes more economically and environmentally viable.

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Passion fruit peel as novel substrate for enhanced β-glucosidases production by Penicillium verruculosum: Potential of the crude extract for biomass hydrolysis

Cited by 38 publications

References 42 publications

The Statistical Optimisation of Recombinant β-glucosidase Production through a Two-Stage, Multi-Model, Design of Experiments Approach

The Statistical Optimisation of Recombinant β-glucosidase Production through a Two-Stage, Multi-Model, Design of Experiments Approach

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

Resíduos agroindustriais como insumos promissores para obtenção de bioprodutos por leveduras - uma breve revisão

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