Passive immunity evaluation in piglets originating from sows vaccinated with China strain of classical swine fever virus

Prodanov, Jasna; Došen, Radoslav; Pušić, Ivan; Bugarski, Dejan; Valčić, Miroslav

doi:10.2298/avb0706413p

Cited by 5 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that 5'UTR region of CSFV was amplified in 10 samples, collected from infected animals on day 6 th and 9 th post infection only. This is in agreement with Jasna et al (2007) who reported that CSFV antigen (viraemia) is detected on 7 th and 9 th dpi by ELISA in piglets of different age group (28, 35, 44 and 54 days old) exposed to contact infection of CSFV and these findings were confirmed by applying RT-PCR technique, the presence of viral RNA was detected only in 3 piglets from blood samples. Hence detectable level of CSFV antigen was observed on day 6-7 th post infection and gradually increased till death.…”

Section: Advances In Animal and Veterinary Sciencessupporting

confidence: 92%

Detection of Classical Swine Fever Virus Antigen and Nucleic Acid on Blood of Experimentally Infected Piglets

Raut¹

2015

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

| Classical swine fever (CSF) is an economically important contagious viral disease, responsible for high mortality in young swine population. Classical swine fever virus (CSFV), the causative agent of CSF, belongs to genus Pestivirus under family Flaviviridae. Early detection of virus /genome is important for employing the disease containment measures. In present study, kinetics of antigen in experimentally infected piglets was determined by antigen ELISA and RT-PCR. Out of 12 sero-negative piglets, six were infected with virulent CSFV by intramuscular route, while other six were kept as control group. Piglets were regularly observed on the days post infection (dpi) for development of clinical manifestations of infection. Blood samples were collected on 0, 3, 6, 9, 12, 15, 18 and 21 dpi or till animal succumb of disease. Samples were screened by commercially available antigen ELISA and RT-PCR, for detection of viral proteins and nucleic acid to confirm the disease, prior to onset of clinical signs. Typical symptoms of disease were observed in the infected piglets on 10-12 dpi. Presence of CSFV antigen in blood sample from day's 6 th to7 th post infection and viral nucleic acid on 6 th and 9 th dpi were confirmed by antigen ELISA and RT-PCR respectively. It was concluded by the present study that CSFV antigen can be detected even before the appearance of clinical signs. Such early detection can be used for field samples to test their repeatability and to prove as tools to control the spread of disease and to minimize economic losses.

show abstract

Section: Advances In Animal and Veterinary Sciencessupporting

confidence: 92%

Detection of Classical Swine Fever Virus Antigen and Nucleic Acid on Blood of Experimentally Infected Piglets

Raut¹

2015

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…In infected piglets, course of the disease was influenced by the virulence of the virus and symptoms may vary hence, the antigen ELISA, based on the detection of viral antigen (Depner et al 1995) and molecular technique RT-PCR based become helpful for rapid detection of CSFV (Le Dimna et al 2008). A challenge study revealed that virus in blood can be detected in blood collected on 6-7th day post infection (dpi) by antigen ELISA and RT PCR (Jasna et al 2007, Raut et al 2015, while, typical symptoms of CSF (anorexia, conjunctivitis, in-coordination, diarrhea, reddening of the base of ear etc) were observed 10-12 dpi onwards (Cariolet et al 2008, Raut et al 2015. So, it is very much necessary to create awareness among the pig farmers to submit blood samples along with tissue samples to the reference diagnostic laboratories for diagnosis of CSF.…”

Section: Resultsmentioning

confidence: 99%

Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR

Choori

Patil

RATNAMMA

et al. 2018

Indian J of Anim Sci

View full text Add to dashboard Cite

Classical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmersand pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state andto evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in detecting the disease. Serum sampleswere collected from 270 pigs from 13 districts of the state and were tested for the presence of CSF antibodies.Whole blood samples (151) from 14 outbreaks of CSF were collected for the comparative diagnosis of the diseaseusing Antigen ELISA and reverse transcriptase (RT)-PCR. A seroprevalence of 28.5% (77/270) was found in theserum samples collected form the whole Karnataka. The southern Karnataka had higher seroprevalence (47%) incomparison to northern Karnataka region where seroprevalence was 17%. It confirms endemicity of the disease insouthern region. Of 151 blood samples collected for comparative diagnosis, 61 samples were positive for CSF byRT-PCR and 39 by antigen ELISA, indicating the superiority of RT-PCR over antigen ELISA to detect CSFVinfection in earliest stages of infection.

show abstract

Expression of E2 (Gp 55) Glycoprotein of Classical Swine Fever Virus in Lymphoid Tissue and Brain of Experimentally Infected Piglets with Different Immunological Status

et al. 2014

View full text Add to dashboard Cite

Classical swine fever (CSF) is a highly contagious viral disease of domestic swine and wild boars. The aim of the study was to examine samples of lymphoid tissues and brain for the presence of Classical Swine Fever Virus (CSFV) antigen in piglets that originate from vaccinated and unvaccinated sows, in order to compare the intensity of pathological lesions in conditions of different immunological status. A total of 20 crossbreed piglets of both sexes, aged 45 days were divided in three groups (G1, G2 and G3). Piglets of G1 originated from sows vaccinated with a live vaccine containing attenuated C strain CSFV. Piglets of G2 originated from unvaccinated sows. Two piglets of G3 originated from vaccinated and two piglets from unvaccinated sows and they served as the control group. All the piglets from G1 and G2 were intramuscularly inoculated with 2×10 5 TCDI/ 50 of CSFV, Baker strain and they died by the 22 nd day post inoculation. Immunohistochemistry was applied for immunolabeling E2 (gp55) glycoprotein of CSFV in the tonsils, mandibular lymph nodes, ileocecal valve and brain of all tested piglets. Differences were observed in the severity of lesions in the lymphoid tissue and the brain between G1 and G2 piglets. The present level of colostral antibodies was not able to protect piglets in G1 from the fatal outcome of the disease. Among others, this fi nding may also have an impact on vaccination policy in the future. Having in mind that vaccination of all domestic pigs with attenuated C-strain vaccine is still a mandatory control measure for CSF in Serbia, soon a non-vaccination policy should be harmonized with EU directives.

show abstract

Passive immunity evaluation in piglets originating from sows vaccinated with China strain of classical swine fever virus

Cited by 5 publications

References 10 publications

Detection of Classical Swine Fever Virus Antigen and Nucleic Acid on Blood of Experimentally Infected Piglets

Detection of Classical Swine Fever Virus Antigen and Nucleic Acid on Blood of Experimentally Infected Piglets

Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR

Expression of E2 (Gp 55) Glycoprotein of Classical Swine Fever Virus in Lymphoid Tissue and Brain of Experimentally Infected Piglets with Different Immunological Status

Contact Info

Product

Resources

About