Patch testing with thin‐layer chromatograms

Bruze, M.; Frick, Mona; Persson, Lena

doi:10.1034/j.1600-0536.2003.00009.x

Cited by 60 publications

(38 citation statements)

References 2 publications

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“…Thereafter, the TLC sheets were cut into strips (one strip for each application) to be used for patch testing, and the positions of the spots were marked on the plastic backing of the TLC strips with a marker pen. One strip of each chromatogram was used as a template for reading of the test, and at least one strip was left to be used for chemical investigations of areas giving positive test reactions . Overall, five patients were tested with TLC strips of the adhesive patch and the sensor.…”

Section: Methodsmentioning

confidence: 99%

Unexpected positive patch test reactions to sesquiterpene lactones in patients sensitized to the glucose sensor FreeStyle Libre

et al. 2019

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Background: Most diabetic patients sensitized to FreeStyle Libre react to isobornyl acrylate (IBOA), with a considerable number of them also showing unexpected positive patch test reactions to sesquiterpene lactone (SL) mix (SLM) tested in the baseline series. Objectives: To compile patch test results of subjects affected, and provide potential explanations for this association. Patients and Methods: Fifty-three Freestyle Libre-allergic patients were patch tested with IBOA and/or SLM, and several were also patch tested with the components of SLM. Chromatographic analyses were performed on the glucose sensor, IBOA, and the components of SLM. Results: Thirty-three patients reacted positively to the components of SLM, and 11 of 27 patients reacted positively to alantolactone, in particular. Gas chromatography-mass spectrometry (GC-MS) analyses did not detect these chemicals in the different parts of the glucose sensor, or in IBOA. Conclusion: Significant co-sensitizations between SLs on the one hand and the glucose sensor FreeStyle Libre and/or isobornyl acrylate on the other hand exist, without evidence of the presence of SLs via GC-MS analysis. Cross-reactions between them seem improbable. As a possible hypothesis, a common precursor for both, such as camphene, may exist. K E Y W O R D S allergic contact dermatitis, chemical analysis, diabetes mellitus, FreeStyle Libre, GC-MS, glucose sensor, isobornyl acrylate, medical device, sesquiterpene lactones, simultaneous reactions

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Section: Methodsmentioning

confidence: 99%

Unexpected positive patch test reactions to sesquiterpene lactones in patients sensitized to the glucose sensor FreeStyle Libre

et al. 2019

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“…The sofas were traced back to the Chinese manufacturer (Linkwise). Collaboration between the UK, the Finnish dermatologists and the Swedish chemical analysis experts in Malmö was established 2 …”

Section: Ukmentioning

confidence: 99%

An outbreak of furniture related dermatitis (‘sofa dermatitis’) in Finland and the UK: history and clinical cases

Susitaival

Winhoven

Williams

et al. 2010

Acad Dermatol Venereol

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In February 2007, an epidemic of severe dermatitis from Chinese recliner chairs and sofas started to unfold first in Finland and a few months later in the UK. Some patients reacted in patch tests (PTs) strongly to the material of their furniture, either leather or fabric. There have been hundreds of reports of chair or sofa dermatitis from Finland and the UK, with all cases linked to the same furniture factory in China. Clinical findings in both countries were very similar and unlike any known dermatosis. Many cases have been quite severe, resembling mycosis fungoides or septic infections, requiring hospitalization. Commercial PTs did not reveal the cause but a fungicide was strongly suspected, although such use was denied by the factory. The laboratory of Malmö University Dermatology Clinic has helped in the process by making thin layer chromatograms from sofa or chair materials and test substances of suspected chemicals. Finally, sachets marked with 'mouldproof agent' were found in varying numbers and distribution in the sofas. These contained dimethyl fumarate (DMF) which proved in skin tests to cause strong positive reactions with down to 0.01 dilution. Reports from other countries (Belgium, France, Ireland, Sweden and Spain) have since appeared, and the EU has banned the use of DMF in consumer products.

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“…Subsequently, patch tests with thin-layer chromatograms were carried out (the chromatograms, made with 50 AL and 100 AL of rubber extract, were also developed at the laboratory of Prof. Bruze). 10 Positive results (+++) at 48 and 96 hours were shown by both chromatograms (Fig 4).…”

Section: Case Reportmentioning

confidence: 82%

“…11 Accordingly, some authors suggest performing patch tests with the extracts obtained by chromatography in order to find the allergen responsible for the lesions. 10 We have described a new case of allergic contact dermatitis from the amine antioxidants in black rubber, indicated by (1) positive patch-test reactions to PPD mix and its components IPPD, DPPD, and CPPD; (2) a positive reaction to IPPD in the chemical analysis of the black rubber container that our patient used in his work; and (3) a positive patch-test reaction to a black rubber thin-layer chromatogram. We would like to emphasize the feasibility and usefulness of patch-testing with chromatography plates in selected cases.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Occupational Allergic Contact Dermatitis from Antioxidant Amines in a Dental Technician

Condé‐Salazar¹,

Valks²,

Acebes³

et al. 2004

Dermatitis

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We describe an 18-year-old dental technician who presented with dry hyperkeratotic lesions on his left palm that were limited to an area that was in contact with a container in which he had prepared the molds for a dental prosthesis. On patch testing, he had a positive reaction to black rubber mix and its components N-cyclohexyl-NV-phenyl-4-phenylenediamine, N,NV-diphenyl-4-phenylenediamine, and N-isopropyl-NV-phenyl-4-phenylenediamine, as well as to a piece of the rubber container. Thin-layer chromatography (TLC) of a piece of the rubber container confirmed the presence of the para-phenylenediamine mix. Results of patch-testing with TLC were positive after 48 and 96 hours. The lesions resolved when the patient stopped using the container. The patient was diagnosed with occupational allergic contact dermatitis. We also review the dermatoses caused by antioxidant amines.

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Patch testing with thin‐layer chromatograms

Cited by 60 publications

References 2 publications

Unexpected positive patch test reactions to sesquiterpene lactones in patients sensitized to the glucose sensor FreeStyle Libre

Unexpected positive patch test reactions to sesquiterpene lactones in patients sensitized to the glucose sensor FreeStyle Libre

An outbreak of furniture related dermatitis (‘sofa dermatitis’) in Finland and the UK: history and clinical cases

Occupational Allergic Contact Dermatitis from Antioxidant Amines in a Dental Technician

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