Pathogenesis of Salmonella enterica serovar albany in experimental infected SPF BALB/c Mice

Abstract

Salmonellosis remains an important zoonotic disease and public health concern, Salmonella enterica serovar Albany is one of the motile serovars which has been identified from poultry and humans. However, its pathogenic potentials and shedding probability and duration from infected/colonized chickens have never been reported. To assess its pathogenic potentials and shedding probability 6 SPF BALB/c mice was inoculated with 0.1ml volume for each mice bacterial solution of 10 8 CFU/ml of Salmonella entrica serovar Albany after 24 hours the segments of the duodenum, jejunum, ileum, caecum and colon were fixed to study the histopathology and the polymerase chain reaction (PCR) was used to confirm the Salmonella entrica serovar Albany in the intestinal mucus swabs. The control group consist of 6 SPF BALB/c mice were inoculated with 0.1ml of 0.9% normal saline, The pathogenesis incidence rate of the disease caused by S. entrica serovar Albany revealed that prominent blood vessels on caecum 100%, red intestinal serosal 100%, infiltration of inflammatory cells in the crypt of liberkhun and submuscular layer of small intestine 100%, enterocyte necrosis 100%, haemorrhagic enteritis 83.3%, cecitis 33.3%, colonitis 66.6%, villus atrophy 100%, crypt atrophy 100%, and detachment of epithelial tissue 50%, can occur as soon as 24 hours post infection. Infected S. entrica serovar Albany was also successfully re-isolated from the intestinal swabs which revealed that the mice is potentially shed the bacteria through feces.

Antimicrobial activity of peptides extracted from camels' blood neutrophils against some pathogenic bacteria

“…The two-stage radial diffusion method was used for it is sensitive and important when studying the inhibiting effect of the isolated cationic peptides from camels' blood on two kinds of pathogenic bacteria (Salmonella typhimurium and Bacillus subtilis) which are known as resistant of antibiotics drugs as shown in table 1. Salmonella typhimurium is one of the bacterial serovars responsible for human and animal infections, as well as noted the increasing rate of antibiotic resistance of Salmonella typhimurium, and the ability to form endospore by Bacillus subtilis allows increasing of bacterial resistance and survival (21)(22)(23). These peptides have a positive charge that reacts and attracts the negative charge bacterial cell membranes and cell walls, as well as the presence of special receptors for these peptides on the bacterial cell walls.…”

Estimation of limit of detection of Salmonella typhimurium in artificially contaminated chicken meat by cultured-based and polymerase chain reaction techniques

“…The pair of the primers used to find Salmonella at genus level codifies the gene InvA that is widely distributed in Salmonella spp. (5,20,21). In addition, the reference procedure of the ISO numbered 6579-1:2017 used as a complementary test to PCR for detection the limit of salmonella conducted for all the examined samples.…”

“…Salmonella is one of the most important pathogen that can infect human through ingestion of contaminated food with a sub-classification of more than 2500 serovars including Typhimurium related to foodborne salmonellosis in human (1,2). This infection can be harsh particularly in immunosuppressive people like youngest or elderly people.…”

Molecular Characterization of Non-typhoidal Salmonella (NTS) Isolated from Salad Vegetables in Terengganu, Malaysia

“…This finding could be of concern as these S. Albany have the probability to cause disease with the presence of considerable numbers of virulence traits. Furthermore, Sabri et al [25] have demonstrated the pathogenesis of S. Albany by experimental mice which revealed the occurrence of haemorrhagic enteritis, cecitis and colonitis as soon as 24 hours after infection.…”