Pathogenicity and complete genome sequence of a fowl adenovirus serotype 8 isolate

Grgić, Helena; Yang, Danhui; Nagy, Éva

doi:10.1016/j.virusres.2011.01.002

Cited by 82 publications

(66 citation statements)

References 38 publications

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“…A second peak of viral genome copy numbers in the liver and bursa of Fabricius samples from the wtFAdV-9 group occurred at 5 d.p.i., which was well in accord with the highest titer detected at that time point. Similar trends have been seen for FAdV-8 (8). Viral genome copy numbers, indicating the virus load in different tissues, were significantly higher in the wtFAdV-9 group than in the FAdV-9⌬4 group tissue samples except in spleen and were shown to be the highest in the cecal tonsil samples.…”

Section: Discussionsupporting

confidence: 78%

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Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Deng

Sharif

Nagy

2013

Clin Vaccine Immunol

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Fowl adenoviruses (FAdVs) are a potential alternative to human adenovirus-based vaccine vectors. Our previous studies demonstrated that a 2.4-kb region at the left end of the FAdV-9 genome is nonessential for virus replication and is suitable for the insertion or replacement of transgenes. Our in vivo study showed that the virus FAdV-9⌬4, lacking six open reading frames (ORFs) at the left end of its genome, replicates less efficiently than wild-type FAdV-9 (wtFAdV-9) in chickens that were infected intramuscularly. However, the fecal-oral route is the natural route of FAdV infection, and the oral administration of a vaccine confers some advantages compared to administration through other routes, especially when developing an adenovirus as a vaccine vector. Therefore, we sought to investigate the effects of FAdV-9 in orally inoculated chickens. In the present study, we orally inoculated specific-pathogen-free (SPF) chickens with FAdV-9 and FAdV-9⌬4 and assessed virus shedding, antibody response, and viral genome copy number and cytokine gene expression in tissues. Our data showed that FAdV-9⌬4 replicated less efficiently than did wtFAdV-9, as evidenced by reduced virus shedding in feces, lower viral genome copy number in tissues, and lower antibody response, which are consistent with the results of the intramuscular route of immunization. Furthermore, we found that both wtFAdV-9 and FAdV-9⌬4 upregulated the mRNA expression of alpha interferon (IFN-␣), IFN-␥, and interleukin-12 (IL-12). In addition, there was a trend toward downregulation of IL-10 gene expression caused by both viruses. These findings indicate that one or more of the six deleted ORFs contribute to modulating the host response against virus infection as well as virus replication in vivo.

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Section: Discussionsupporting

confidence: 78%

“…To date, the genomes of four fowl adenoviruses (those of FAdV-1, FAdV-9, FAdV-8, and FAdV-4) have been fully sequenced (6)(7)(8)(9), and they are about 10 kb larger than those of mastadenoviruses.…”

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confidence: 99%

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Deng

Sharif

Nagy

2013

Clin Vaccine Immunol

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“…Turkey enteric coronavirus is associated with PEMS and turkeys inoculated with coronavirus and E. coli developed lymphocyte necrosis and depletion in the bursa of Fabricius (Kang et al, 2012). Thymus atrophy is associated with PEMS, and it occurs in turkeys inoculated with Astrovirus (Grgić et al, 2011). A small round virus, possibly an enterovirus or Astrovirus, can replicate in lymphoid tissues of turkeys, causing lymphocyte necrosis and depletion of lymphoid organs, and corresponding reductions in lymphocyte subpopulations in circulating blood (Hoerr, 2010).…”

Section: Marek's Disease and Enteric Disease Virusesmentioning

confidence: 99%

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

Umar

Munir

Ahsan

et al. 2017

World's Poultry Science Journal

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Poultry can be exposed to different kinds of immunosuppressive agents that impair health and welfare by destroying innate and acquired immunity leading to diminished genetic potential of poultry for efficient production. Immunosuppression is a condition characterised by humoral and cellular immune dysfunction that leads to increased susceptibility to secondary infections and vaccine failure. Immune dysfunction at the humoral level is largely due to change in soluble factors mediated by complement or chemokines for innate immunity or due to alterations in antibodies or cytokines for adaptive immunity. In contrast, immune dysfunctions at cellular levels include alterations in neutrophils, monocyte/macrophage, and natural killer cells for innate immunity or changes in B or T lymphocytes for adaptive immunity. In poultry, stressinduced immunosuppression is manifested by failure in vaccination, and increased morbidity and mortality of flocks. Immunosuppressive agents can have cytolytic effects on lymphocyte populations leading to atrophied and depleted lymphoid organs. Immunosuppression can be due to infectious agents or noninfectious agents or due to a combination of them. At present, several modern cellular and molecular approaches are being used to determine the status of the immune system during stress and disease. Comprehensive methodologies for the evaluation of immunosuppression by combined non-infectious and infectious aetiologies have not found general application. Currently, investigations are being developed in order to detect genetic expression of immunologic mediators and receptors by microarray technology. It is likely that this new technique will initiate the development of new strategies for the control and prevention of immunosuppression in poultry. A long term immunosuppression preventive approach involves genetic selection for resistance to immunosuppressive diseases.

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“…Among these manifestations, the egg drop syndrome in layers and Inclusion body hepatitis along with hydropericardium syndrome (IBH-HPS) in broilers (Grgic et al, 2011) are frequently encountered. IBH-HPS is caused by fowl adenovirus serotype-4 in broiler chickens, which mainly affects age group of 3 to 6 weeks old.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

Ali¹,

Mahmood²,

Hussaın³

et al. 2015

IJAB

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The current study was conducted to prepare and evaluate the efficacy of live attenuated vaccine of IBH-HPS in broiler birds in Pakistan. The IBH-HPS virus was successfully adapted after three blind passages (Vero cell line) and the 4 th passage was fully adapted as it gave cytopathogenic effect (CPE) in 48 h post infection in Vero cells. The 9 th passage was determined for attenuation in broiler birds. The attenuated virus was subjected to pathogenecity testing and used as vaccine candidate in broiler chickens. Two hundred and fifty, day-old commercial broiler chicks were purchased from local market, divided into 5 groups and offered feed and water ad libitum. At the age of 15 days, 50 birds in each group were administered with 9 th passage live attenuated lyophilized vaccine, the 3 rd passage ß-propiolactone inactivated vaccine (prepared in the laboratory), oil emulsified inactivated vaccine (UAF-Angavac), commercial vaccine (Bio-Angara, Sana Laboratories, Pakistan), and normal saline, respectively as negative control. The 9 th passage live attenuated lyophilized vaccine protected 100% birds from mortality, clinical signs and high antibody titre was observed. On the other hand, ß-propiolactone inactivated vaccine gave 70% protection. The oil emulsified inactivated vaccine gave 80% protection but lower weight gain was observed. The commercial vaccine gave 60% protection. In negative control 70% mortality was recorded. The results revealed that the live attenuated lyophilized vaccine showed a higher protection against lethal challenge of IBH-HPS in broilers.

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Pathogenicity and complete genome sequence of a fowl adenovirus serotype 8 isolate

Cited by 82 publications

References 38 publications

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

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