Pathogenicity of a fowl adenovirus serotype 4 isolated from chickens associated with hydropericardium-hepatitis syndrome in China

Ren, Guangcai; Wang, Han; Yan, Yuanyuan; Fan, Liu; Huang, Miaorong; Chen, Ruiai

doi:10.3382/ps/pez042

Cited by 32 publications

(22 citation statements)

References 26 publications

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“…The genome sizes of all these isolates were similar to those of previously reported Chinese isolates; however, they differed in size from those of the isolates from other countries, including nonpathogenic isolates ( Griffin and Nagy, 2011 ; Liu et al., 2016 ; Vera-Hernández et al., 2016 ; Ye et al., 2016 ; Mo et al., 2019 ; Ren et al., 2019 ). The full genome sizes of isolates GX2017-01, GX2017-02, GX2017-03, GX2017-04, GX2017-05, GX2017-06, GX2018-07, GX2018-08, and GX2019-09 were 43,725, 43,725, 43,723, 43,723, 43,722, 43,718, 43,719, 43,726, and 43,726 bp, respectively.…”

Section: Resultssupporting

confidence: 72%

“…Fowl adenovirus 4 has been isolated, and its complete genome has been sequenced from different parts of the world and China. To date, the complete genome of 44 isolates of FAdV-4 has been sequenced worldwide; so far, only one isolation has been made from Guangxi province, China ( Ren et al., 2019 ). Therefore, more knowledge and study was required from this province, so the complete genome of 9 FAdV-4 isolates was sequenced and genetically characterized from this province.…”

Section: Discussionmentioning

confidence: 99%

“…One h after infection, the cell layer was overlaid with Medium 199 containing 2% agarose (Sigma-Aldrich) and incubated for 3 to 5 D at 37°C in a 5% CO 2 humidified atmosphere. Isolated plaques were then picked and transferred to a new fresh culture of LMH cells ( Ren et al., 2019 ). The detection of virus in the samples was performed by PCR using the primers specific for the FAdV hexon gene (GenBank: GU188428), forward: 5′-CAA CTA CAT CGG GTT CAG GG-3′and reverse: 5′-TGG CGT TTC TCA GCA TCA-3′.…”

Section: Methodsmentioning

confidence: 99%

“…The disease is characterized by the accumulation of clear and straw-colored fluid in the pericardial sac. Other signs included an enlarged and discolored liver with hemorrhage or necrotic foci of hemorrhage and necrosis ( Ren et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of fowl aviadenovirus 4 isolates from Guangxi, China, during 2017–2019

et al. 2020

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Hepatitis-hydropericardium syndrome ( HHS ) is a severe disease that causes 20 to 80% mortality in chickens aged 3 to 6 wk. Fowl aviadenovirus serotype 4 ( FAdV-4 ) plays an important role in the etiology of HHS. Since 2015, outbreaks of HHS have been reported in several provinces of China; however, details regarding the FAdV-4 genome properties are lacking. In the present study, the complete genomes of 9 isolates responsible for these outbreaks in Guangxi Province, China, were sequenced. To investigate the molecular characteristics of these FAdV-4 isolates, we compared their genomes with those of other reported pathogenic and nonpathogenic FAdV-4 isolates. A variable number of GA repeats were observed in the isolates of this study. Each of the isolates GX2017-01, GX2017-02, GX2018-08, and GX2019-09 had 11 GA repeats; GX2017-03, GX2017-04, and GX2017-05 each had 10 GA repeats, while GX2017-06 and GX2018-07 each had 8 GA repeats. We observed several deletions and distinct amino acid mutations in the major structural genes of these isolates when compared with non-Chinese isolates. We found 2 novel putative genetic markers in the hexon protein, one present in GX2017-02, in which aspartic acid (D) was changed to tyrosine (Y), and another present in each of isolates GX2018-08 and GX2019-09, in which serine (S) was changed to arginine (R), when compared with selected Chinese and some non-Chinese isolates. Moreover, the phylogenetic analysis revealed that all the isolates of this study were clustered within FAdV-C. We found that these isolates were closely related to other recently isolated Chinese strains. The data presented in this study will not only increase the understanding of the molecular epidemiology and genetic diversity of FAdV-4 isolates in China but also has an important reference value of the major factors that determine the virulence of FAdV-4 strains.

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Section: Resultssupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of fowl aviadenovirus 4 isolates from Guangxi, China, during 2017–2019

et al. 2020

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“…In addition, there are also reports of viral co-infection, such as by duck circovirus (DuCV) and duck hepatitis virus 1 (DHV-1) [18]; H9N2 AIV and Tembusu virus (TMUV) [19]; Marek’s disease virus (MDV) and CIAV [20]; and duck hepatitis A virus (DHAV-1) and DHAV-3 [21]. Currently, although FAdV-4 can clearly cause HHS on its own in chickens and ducks [22,23,24,25], both the extent of the FAdV-4 infection and degree of co-infection in the area remain unclear. Therefore, to better understand the prevalence of FAdV-4 and the incidence of co-infection by immunosuppressive viruses, an epidemiological survey of FAdV-4 infection in chickens and ducks with HHS in the province was conducted.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Fowl Adenovirus Serotype 4 and Co-Infection by Immunosuppressive Viruses in Fowl with Hydropericardium Hepatitis Syndrome in Shandong Province, China

Lin

et al. 2019

Viruses

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Fowl adenovirus serotype 4 (FAdV-4) is the pathogenic agent of hydropericardium hepatitis syndrome (HHS) in chickens and ducks, which has caused huge economic losses for the Chinese poultry industry since 2015. In order to objectively determine the prevalence and co-infection status of the virus in Shandong province in China, we analyzed a total of 679 clinical cases of chickens and ducks from 36 farms in the province. The results showed that the FAdV-4 infection rate was 65.2% (443/679), and the rate in breeder ducks was almost two-fold higher than that in breeder chickens (68.57% vs. 34.30%). Notably, co-infection by H9N2 avian influenza virus, infectious bursal disease virus, and/or chicken infectious anemia virus was very common in the 443 FAdV-4-positive cases. Furthermore, phylogenetic analysis of the hexon genes of four Shandong FAdV-4 isolates revealed that these strains clustered into Indian reference strains, indicating that the Shandong FAdV-4 strains might have originated in India. These findings provide the first data on the prevalence and co-infection status of FAdV-4 in Shandong province, which may serve as a foundation for the prevention of FAdV-4 in the field.

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Genomic characterization of a fowl adenovirus serotype 4 strain isolated from a chicken with hydropericardium syndrome in Japan

et al. 2022

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Pathogenicity of a fowl adenovirus serotype 4 isolated from chickens associated with hydropericardium-hepatitis syndrome in China

Cited by 32 publications

References 26 publications

Genetic characterization of fowl aviadenovirus 4 isolates from Guangxi, China, during 2017–2019

Genetic characterization of fowl aviadenovirus 4 isolates from Guangxi, China, during 2017–2019

Prevalence of Fowl Adenovirus Serotype 4 and Co-Infection by Immunosuppressive Viruses in Fowl with Hydropericardium Hepatitis Syndrome in Shandong Province, China

Genomic characterization of a fowl adenovirus serotype 4 strain isolated from a chicken with hydropericardium syndrome in Japan

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