Pathophysiology of acute wound healing

Li, Jie; Chen, Juan; Kirsner, Robert S.

doi:10.1016/j.clindermatol.2006.09.007

Cited by 3,014 publications

(1,145 citation statements)

References 57 publications

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“…Fast epithelial regeneration and great number of MNLs increase the fibroblast and collagen reproduction in the wound area through the secretion of growth hormones and cytokines during healing period. [38][39][40] There was no significant difference between groups in the length of wound epithelium within the first 7 d (pϾ0.05), however, the longest new epithelium formation was observed in the group treated with fucoidan-chitosan hydrogel on days 14 (2358Ϯ40 mm) (pϽ0.05) and 21 (5566Ϯ555 mm) (pϽ0.05), as shown in Table 3. Wound epithelium thickness values in the fucoidan-chitosan hydrogel group, which was measured as 162Ϯ10 mm, 261Ϯ16 mm and 189Ϯ23 mm on days 7, 14 and 21 respectively, was shown to be significantly higher during healing period when compared to other groups (Table 3) (pϽ0.05).…”

Section: Resultsmentioning

confidence: 91%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

160

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Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

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Section: Resultsmentioning

confidence: 91%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

160

View full text Add to dashboard Cite

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“…However, in comparison with the SP group, a significant difference was not found. Epithelization was realized via wound contractions and remodelling EGF 37 . In the studies, these collective biological characteristics are said to support the clinical use of platelet derivatives in providing haemostasis and assisting in the process of healing 38,39 .…”

Section: Discussionmentioning

confidence: 99%

Is there any association between mycosis fungoides and Th-1, Th-2 cytokines and transforming growth factor-beta 1 gene polymorphisms?

Yazıcı¹,

Aydoğan²,

Sarıcaoğlu³

et al. 2018

TURKDERM

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“…Since fibroblast activation and collagen production can be affected by keratinocyte-secreted factors and/or inflammation, 3,30 we examined whether keratinocyte-specific Smad7 induction affects type-I collagen production. In situ hybridization shows fewer COL1A1 mRNA-positive cells in the wound area of Smad7 tg skin compared to control skin wounds at days 5, 7, and 11 after wounding ( Figure 7A).…”

Section: Smad7 In Wound Healingmentioning

confidence: 99%

“…6 Reduced inflammation in Smad7 tg wounds could be responsible, at least in part, for reduced angiogenesis and collagen production, as both occurred after the re-duction in inflammation was first observed. Many inflammatory cytokines and chemokines are released by leukocytes, which are angiogenic and fibrogenic, 3,30 could be reduced in Smad7 wounds as a result of reduced leukocyte infiltration. As a result, early-stage angiogenesis and collagen production required for wound repair were not perturbed, whereas prolonged angiogenesis and collagen production were prevented in Smad7 tg wounds.…”

Section: Stromal Effects Of Epithelial-specific Smad7 Overexpression mentioning

confidence: 99%

Temporal Smad7 Transgene Induction in Mouse Epidermis Accelerates Skin Wound Healing

Han

Dijke

et al. 2011

The American Journal of Pathology

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The expression of Smad7, a tumor growth factor-␤ (TGF␤) antagonist, is increased during cutaneous wound healing. To assess this significance, we temporally induced Smad7 transgene expression in wounded skin in gene-switch-Smad7 transgenic (Smad7 tg) mice. Smad7 induction in epidermal keratinocytes caused an increase in keratinocyte proliferation with reduced Smad2 activation, indicating that Smad7 abrogated TGF␤-mediated growth inhibition. Additionally, wounded skin from Smad7 tg mice exhibited accelerated re-epithelialization, with increased activation of extracellular signal-regulated kinase (Erk), and an in vitro migration assay revealed that Erk activation contributed to Smad7-mediated keratinocyte migration. Notably, epidermis-specific Smad7 transgene expression also has a profound effect on the wound stroma, resulting in reduced inflammation, angiogenesis, and production of type I collagen. Reduced Smad2 activation was observed in wounded stroma from Smad7 transgenic (Smad7 tg) mice, possibly owing to fewer infiltrated TGF␤-producing leukocytes compared to those in wounds from control mice. Because Smad7 is not secreted, these effects could reflect functional changes in Smad7 tg keratinocytes. Supporting this notion, the activation of NF-B, a nonsecreted protein complex that transcriptionally activates inflammatory cytokines, was reduced in wounded epidermis from Smad7 tg mice compared to that in wounded wildtype epidermis. In sum, epidermal Smad7 overexpression accelerated wound healing through its direct effects on keratinocyte proliferation and migration, and through indirect effects on wound stroma.

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Pathophysiology of acute wound healing

Cited by 3,014 publications

References 57 publications

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Is there any association between mycosis fungoides and Th-1, Th-2 cytokines and transforming growth factor-beta 1 gene polymorphisms?

Temporal Smad7 Transgene Induction in Mouse Epidermis Accelerates Skin Wound Healing

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