Pathophysiology of the pancreas after oral infection of genetically diverse mice with coxsackievirus B4-E2

Precechtelova, Jana; Borsanyiova, Maria; Stipalova, D.; Šarmírová, Soňa; Gomolcák, P; Berakova, Katarina; Bopegamage, Shubhada

doi:10.1007/s00705-014-2236-7

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…IFN-α and virus VP1 were still present long after infection in some (not all) mice. Later on, we studied (Precechtelova et al, 2015) oral infection of different mouse strains with different genetic backgrounds with CVB4-E2. We used 1) outbred (Swiss albino and CD1), 2) inbred (SJL, non-obese diabetic [NOD]), and 3) transgenic (NOD.SCID) mice.…”

Section: Our Studies In the Murine Model Using The Oral Infectionmentioning

confidence: 99%

Murine experimental models for studying the pathogenesis of coxsackieviruses

Bopegamage¹,

Benkoova²,

Pospíšilová³

et al. 2020

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Understanding the pathogenesis of communicable diseases often involves animal models. Mouse models are studied by researchers to achieve a better understanding of the relationship between the biological, physiochemical, and antigenic properties of the infectious agent, as well as the histopathological, immunological, and functional changes in the living system and the target organs, in short, the pathophysiological processes that the communicable agents bring about. The long-term objectives of the in vivo studies are important from a medical point of view, as they represent faithful (reliable and similar) human responses, which enhance the development of diagnostics, treatment, and measures for preventing the spread of the disease. Our work is devoted to the murine models used for understanding the pathogenesis of coxsackieviruses. We describe different mouse models used for studying the diseases caused by coxsackieviruses and the immune responses in different mouse models. We then shortly elucidate experiences from our laboratory related to the oral route of infection, and compare the similarities and differences we found in this model.

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Section: Our Studies In the Murine Model Using The Oral Infectionmentioning

confidence: 99%

Murine experimental models for studying the pathogenesis of coxsackieviruses

Bopegamage¹,

Benkoova²,

Pospíšilová³

et al. 2020

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“…Other reagents used were the secondary antibody included in the Dako ARK (Animal Research Kit) and peroxidase for mouse secondary antibodies (Dako). The methodological details are described in our previous studies [57,58].…”

Section: Rna Extractionmentioning

confidence: 99%

Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue

et al. 2019

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Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology and immunohistochemical (IHC) analysis for localization and identification of Ly6G+ cells (neutrophils), CD11b+ cells (macrophages), and viral protein in pancreatic tissue sections of the infected dams and their challenged pups. Our results show the presence of replicating virus in the pancreas of infected dams and their challenged pups, with inflammation leading to chronic necrotizing pancreatitis and atrophy of pancreatic acini of the dams and their offspring. IHC analysis of the infiltrating cells showed pronounced Ly6G+ neutrophils in dams only, whereas CD11b+ macrophages were present in tissues of both, the pups and the dams. Time of infection during gravidity as well as the p.i. intervals when mice were sacrificed influenced the pancreatic pathophysiology in both groups. We conclude that coxsackievirus infection during pregnancy is a risk factor for chronic affliction of the exocrine tissue and could affect endocrine pancreas in the mother and child.

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“…The CD1 mouse strain is derived from a Swiss stock and is of great interest for toxicology and virology since its genetic structure is close to that of natural Mus musculus populations. CD1 mice are susceptible to diabetogenic viruses such as EMCV and CV-B4 26 . The purpose of the present study was to investigate the impact of CV-B4E2 combined with a single sub-diabetogenic dose of streptozotocin on the pancreas and on pancreatic β cells of outbred mice.…”

Section: Introductionmentioning

confidence: 99%

Impact of coxsackievirus-B4E2 combined with a single low dose of streptozotocin on pancreas of outbred mice: investigation of viral load, pathology and inflammation

Benkahla

Sané

Bertin

et al. 2019

Sci Rep

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Coxsackieviruses B (CV-B) belong to the EV-B species. CV-B and particularly CV-B4 are thought to be involved in the development of chronic diseases like type 1 diabetes (T1D). The mechanisms of the enteroviral pathogenesis of T1D are not well known, yet. The in vitro studies are rich with information but in vivo infection models are needed to investigate the impact of viruses onto organs. Our objective was to study the impact of CV-B4E2 combined with a single sub-diabetogenic dose of streptozotocin (STZ) on the pancreas of mice. The infection with CV-B4E2 of CD1 outbred mice treated with a sub-diabetogenic dose of STZ induced hyperglycemia and hypoinsulinemia. Along with the chemokine IP-10, viral RNA and infectious particles were detected in the pancreas. The pancreas of these animals was also marked with insulitis and other histological alterations. The model combining STZ and CV-B4E2 opens the door to new perspectives to better understand the interactions between virus and host, and the role of environmental factors capable, like STZ, to predispose the host to the diabetogenic effects of enteroviruses.

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Pathophysiology of the pancreas after oral infection of genetically diverse mice with coxsackievirus B4-E2

Cited by 5 publications

References 34 publications

Murine experimental models for studying the pathogenesis of coxsackieviruses

Murine experimental models for studying the pathogenesis of coxsackieviruses

Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue

Impact of coxsackievirus-B4E2 combined with a single low dose of streptozotocin on pancreas of outbred mice: investigation of viral load, pathology and inflammation

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