Pathophysiology of vascular endothelium and circulating platelets: implications for coronary revascularisation and treatment

Amoroso, Giovanni; Veldhuisen, Dirk J. van; Tio, René A.; Mariani, Mario

doi:10.1016/s0167-5273(01)00448-x

Cited by 10 publications

(6 citation statements)

References 77 publications

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“…Maintenance of vessel function and, in particular, endothelial cell function seems important because endothelial injury during storage may promote later vessel thrombosis and vessel wall inflammation 7,8 and induce graft failure. Disturbance in vascular tone regulation is associated with an increased risk for clinical events.…”

mentioning

confidence: 99%

Functional, morphologic, and molecular characterization of cold storage injury

Ebner

Poitz

Augstein

et al. 2012

Journal of Vascular Surgery

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mentioning

confidence: 99%

Functional, morphologic, and molecular characterization of cold storage injury

Ebner

Poitz

Augstein

et al. 2012

Journal of Vascular Surgery

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“…At sites of vascular injury, aggregating platelets release factors that have vasoactive and mitogenic properties (2,14,34). In the absence of intact or functional endothelium, these factors cause contraction of vascular smooth muscle (32,41), recruitment of more platelets and other blood cells, cholesterol synthesis (13), low-density lipoprotein receptor expression (45), and proliferation and migration of vascular smooth muscle cells (SMCs) from the media to the intima layer of the vessel wall (11,15).…”

mentioning

confidence: 99%

Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle

Bracamonte¹,

Rud²,

Owen

et al. 2002

American Journal of Physiology-Heart and Circulatory Physiology

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-Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGFAB and PDGFBB), transforming growth factors (TGF-␤1 and TGF-␤2), and vascular endothelial growth factor (VEGF165) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGFBB, but not PDGFAB, TGF-␤1, and TGF-␤2, increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGFBB in platelets. Higher levels of PDGFBB in platelets in synergy with other plateletderived products could contribute to increased proliferative arterial response to injury after ovariectomy.

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“…The SEM observation indicated that the seeded endothelial cells adhered to the stent and formed a complete layer that could prevent platelet and leukocyte adhesion from the blood. Endothelialization could further reduce neointimal hyperplasia 24, 25. We believe that this is an important step in endothelial cell delivery, although the long‐term effect of heparinized polymer coated stents needs further study.…”

Section: Discussionmentioning

confidence: 98%

Development of a novel endothelial cell‐seeded endovascular stent for intracranial aneurysm therapy

Zhu

Tian

Zhou

et al. 2007

J Biomedical Materials Res

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The metallic stent has been widely used in endovascular treatment of intracranial aneurysms and arterial stenosis. Endothelialization at the neck of the aneurysm or stenotic lesion after stent deployment plays a pivotal role in preventing aneurysm recurrence, as well as local thrombus formation and restenosis. To deliver autologous endothelial cells and to promote the endothelialization on the luminal wall of the parent artery, we established an endothelial cell-seeded intracranial stent device. Endothelial cells were isolated from canine jugular vein and identified by FACS assay and immunohistochemistry. We demonstrated that the seeded endothelial cells formed a confluent endothelial layer on the stent's surface. After being brushed with 100 dyne/cm(2) of shear stress, we found that this endothelial layer remained intact for at least 48 h on the heparinized polymer coated stent, rather than the poly-lactic-acid coated stent (p < 0.05). The results suggest that an autologous endothelial cell-seeded stent may be a feasible and optimal tool for endothelial delivery during stenting and may overcome some limitations of the traditional bare stent in the treatment of intracranial aneurysms and arterial stenosis.

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Pathophysiology of vascular endothelium and circulating platelets: implications for coronary revascularisation and treatment

Cited by 10 publications

References 77 publications

Functional, morphologic, and molecular characterization of cold storage injury

Functional, morphologic, and molecular characterization of cold storage injury

Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle

Development of a novel endothelial cell‐seeded endovascular stent for intracranial aneurysm therapy

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