PBMCs from both atopic asthmatic and nonatopic children show a TH2 cytokine response to house dust mite allergen

Nurse, Barbara; Puterman, A.; Haus, Matthias; Berman, Dilys; Weinberg, Eugene; Potter, Paul

doi:10.1067/mai.2000.107397

Cited by 20 publications

(17 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, allergens induce T cell proliferative responses in the majority of sensitized subjects; however, the magnitude of the responses varies considerably between different individuals. Furthermore, though the magnitude of T cell proliferative responses can be higher in sensitized compared with non-sensitized individuals, this is highly variable, particularly among patients with very high titers of circulating IgE ab such as subjects with atopic dermatitis [36][37][38][39][40][41][42][43]. It has been argued that T cell responses to ubiquitous allergens such as those derived from house dust mites are universal [43].…”

Section: Relevance Of the Th1/th2 Paradigm To Allergic Disease In Manmentioning

confidence: 99%

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Woodfolk

2006

CPD

View full text Add to dashboard Cite

Production of IgE antibodies promotes the development of allergic disorders such as asthma, rhinitis and atopic eczema. Though Th2 cytokines play a pivotal role in the allergic inflammatory cascade, therapeutic strategies which target these factors have not been curative in clinical trials. In humans, the allergic phenotype encompasses a broad spectrum of diverse clinical entities, which are dictated by genetics as well as the nature of the allergen and the time in life at which allergen is encountered. The disparate findings in animal and human systems highlight the complexity of cytokine-mediated events in allergic responses in man. Different allergic phenotypes cannot be distinguished strictly on the basis of Th1 and Th2 cytokines. These observations coupled with an emerging role for regulatory T cells in modulation of the allergic response warrant re-examination of the cytokine network in allergic disease. This article highlights the challenges of dissecting the role of individual cytokines in the development of allergic responses and manifestation of allergic symptoms. Regulatory T cells have been implicated in modulation of the activity of allergen-specific Th1 and Th2 effector cells and immune outcome; however, the characteristics of these regulatory T cells remain largely undefined. The effects of existing and emerging therapies which target Th1, Th2 and regulatory cytokines on established allergic responses are examined in the context of this new paradigm. Taken together, existing data suggest that a multi-faceted approach, which is tailored to each patient, will be required in order to attain clinical benefit.

show abstract

Section: Relevance Of the Th1/th2 Paradigm To Allergic Disease In Manmentioning

confidence: 99%

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Woodfolk

2006

CPD

View full text Add to dashboard Cite

show abstract

“…Nevertheless, in some patients, IL-5 and IL-4 were undetectable. The lack of IL-5 and IL-4 production in some patients with allergy, already reported, [27][28][29] could be a result of the low frequency of Der p 2-specific T H 2-cell clones in the peripheral blood of HDM patients. 30 IL-5-producing and IL-4-producing T cells accumulate and infiltrate the inflamed bronchial mucosa of patients with allergy.…”

Section: Discussionmentioning

confidence: 82%

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Pacciani

Gregori

Chini

et al. 2010

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

“…When PBMCs from healthy volunteers were activated by Der p 1, the production of IL-5 and TNF-α was not increased, yet the INF-γ production was significantly increased in a dose and time dependent manner (data not shown). The PBMCs from patients with atopic asthma show increased proliferation in response to HDM allergens, and atopic asthmatic PBMCs have enhanced Th2 cytokine responses to HDMs, as compared to those PBMCs from normal subjects (18). In this study, the nasal epithelial cells were activated with HDMs and then they were co-cultured with PBMCs.…”

Section: Discussionmentioning

confidence: 99%

Th2 Responses Elicited by Nasal Epithelial Cells Exposed to House Dust Mite Extract

Shin

2009

Clin Exp Otorhinolaryngol

View full text Add to dashboard Cite

ObjectivesRespiratory epithelial cells are the first site of interaction of allergens with the immune system. The aim of this study was to examine the effect of epithelial cells, which were stimulated with house dust mite (HDM) extracts, on the immune response of peripheral blood mononuclear cells (PBMCs).MethodsPrimary nasal polyp epithelial cells were exposed to dermatophagoides pteronyssinus and dermatophagoides farina for 48 hr, and then the supernatant and cells were collected. After stimulation with HDM extract, the epithelial cells were co-cultured with PBMCs for 72 hr and then the supernatant was collected. We measured the interleukin (IL)-8 and granulocyte-macrophage colony stimulating factor to determine the activation of the epithelial cells. The tumor necrosis factor (TNF)-α, IL-5 and interferon-γ were measured to evaluate the interaction between the epithelial cells and the PBMCs. The mRNA expression of intercellular adhesion molecule 1 (ICAM-1) was assessed using the anti-ICAM-1 antibody.ResultsThe HDM extracts activated the nasal epithelial cells and enhanced the expression of ICAM-1 mRNA and cell membrane ICAM-1. When the activated epithelial cells were co-cultured with PBMCs, the PBMCs produced lager amounts of TNF-α and IL-5. However the cytokine production was not inhibited by pretreatment with ICAM-1 antibody.ConclusionHDM allergens induce allergic inflammation by activating nasal epithelial cells, yet the interaction of the epitheila cells and the PBMCs may not be associated with an ICAM-1 medicated mechanism.

show abstract

PBMCs from both atopic asthmatic and nonatopic children show a TH2 cytokine response to house dust mite allergen

Cited by 20 publications

References 46 publications

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Cytokines as a Therapeutic Target for Allergic Diseases: A Complex Picture

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Th2 Responses Elicited by Nasal Epithelial Cells Exposed to House Dust Mite Extract

Contact Info

Product

Resources

About