PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat

Lafiandra, D.; Tucci, G. F.; Pavoni, A.; Turchetta, T.; Margiotta, B.

doi:10.1007/s001220050405

Cited by 68 publications

(42 citation statements)

References 16 publications

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“…Previous studies found that the missing mechanism was associated with the presence of a premature stop codon within the coding region Wan et al, 2002;Sun et al, 2004). This may be because of the insertion of a transposon-like element in the coding region (Harberd et al, 1987;Lafiandra et al, 1997;Yuan et al, 2009). Lafiandra et al (1997) reported that an ancestral active subunit 1 gene was silenced by the insertion of the 8-kb transposon-like fragment into the linked y-type gene.…”

Section: Hmw-gs Compositionmentioning

confidence: 99%

“…This may be because of the insertion of a transposon-like element in the coding region (Harberd et al, 1987;Lafiandra et al, 1997;Yuan et al, 2009). Lafiandra et al (1997) reported that an ancestral active subunit 1 gene was silenced by the insertion of the 8-kb transposon-like fragment into the linked y-type gene. The cause of the missing Glu-A1a(1) in our study requires further study.…”

Section: Hmw-gs Compositionmentioning

confidence: 99%

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Characterization of agronomic and quality traits and HSW-G5 compositions from the progenies of common wheat (Triticum aestivum L.) with different protein content

Bian¹,

Sun²,

Sun³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. High molecular weight glutenin subunits (HMW-GS) play an essential role in wheat processing quality. In this study, we evaluated the genetic pattern with HMW-GS composition between generations and examined whether agronomic and quality traits were correlated with each other. A wheat (Triticum aestivum L.) cultivar with high protein content and 2 cultivars with low protein content were subjected to a reciprocal cross. Sixteen agronomic and 4 quality characteristics were investigated. A total of 216 seeds from each F2 generation were chosen randomly and analyzed for HMW-GS composition using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Agronomic and quality characteristics were not significantly different between reciprocal crosses, indicating no cytoplasmic effect on the characteristics studied. The separation ratio of 2 HMW-GS loci was 9:3:3:1, indicating no linkage between any 2 loci. The novel HMW-GS N was detected in cultivar R145, which did not follow the Mendelian segregation ratio. A Glu-A1a(1) band was not detected in 1 individual from Tian8901xR145. Average grain weight per spike was significantly correlated with quality characteristics and may be a suitable criterion for selecting high protein content in wheat breeding programs.

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Section: Hmw-gs Compositionmentioning

confidence: 99%

Section: Hmw-gs Compositionmentioning

confidence: 99%

Characterization of agronomic and quality traits and HSW-G5 compositions from the progenies of common wheat (Triticum aestivum L.) with different protein content

Bian¹,

Sun²,

Sun³

et al. 2015

Genet. Mol. Res.

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“…It allows the specific amplification of a target DNA segment using a pair of flanked oligonucleotide primers (D'Ovidio et al, 1990(D'Ovidio et al, , 1994(D'Ovidio et al, , 1995. PCR was used to characterize HMW glutenin genes encoding x-and ytype subunits from common wheat (Tahir et al, 1996;Lafiandra et al, 1997). The aims of this study were to characterize a novel y-type HMW glutenin gene, with unusually low molecular weight, deduce its amino acid sequence, and compare the deduced sequence with previously published sequences of y-type subunits controlled by the D genome.…”

Section: Introductionmentioning

confidence: 99%

Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii

et al. 2005

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An unusually small y-type high molecular weight (HMW) glutenin subunit gene from Triticum tauschii was sequenced. This gene, encoded at the Glu-D t 1 locus was designated 12.4 t and is the smallest HMW glutenin subunit gene described so far in Triticum species. Oligonucleotide primers based on published sequences of HMW glutenin genes were designed to amplify the encoding region and the central repetitive domain of the gene, which produced fragments of 1.4 and 0.85 kb, respectively. PCR products were cloned and sequenced. The derived amino acid sequence was compared with the amino acid sequences of the HMW glutenin subunits Dy12 t , from T. tauschii, and subunits Dy10 and Dy12 of T. aestivum. The amino acid sequence deduced from the nucleotide sequence demonstrated that deletions of hexapeptides and nonapeptides were responsible for the reduction in the size of this HMW glutenin subunit. The estimated molecular weight of the Dy12.4 t subunit, calculated on the basis of the deduced amino acid sequence, was 45,228 Daltons. There were also single amino acid differences in the N-, C-terminal and central repetitive domains of this gene in comparison to the three other y-type subunits encoded at the Glu-D1 locus. The Dy12.4 t subunit showed the highest similarity to the Dy12 subunit present in the hexaploid wheat Chinese Spring.

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“…With respect to Glu-D1 and Glu-A1, several PCR assays have already been developed to detect the 'good-quality' HMW glutenin subunit alleles Glu-D1-1d (Dx-5;D'Ovidio and Anderson 1994;Smith et al 1994;Ahmad 2000;De Bustos et al 2000Radovanovic and Cloutier 2003;Schwarz et al 2003), Glu-A1-1b and Glu-A1-1c (Ax-1 andAx-2*; Lafiandra et al 1997;De Bustos et al 2000Radovanovic and Cloutier 2003), respectively. In the case of the Glu-B1 locus, the HMW subunit pairs GLU-B1-1A (Bx-7) + GLU-B1-2A (By-8), GLU-B1-1A (Bx-7) + GLU-B1-2B (By-9) and GLU-B1-1H (Bx-17) + GLU-B1-2F (By-18) were found to be correlated with good breadmaking quality, whereas the combination GLU-B1-1D (Bx-6) + GLU-B1-2A (By-8) is associated with poor breadmaking properties (Branlard and Dardevet 1985;Payne et al 1987;Lawrence et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat

2004

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Polymorphisms between the coding sequences of high-molecular-weight (HMW) glutenin x-type genes at the Glu-1 locus were used to amplify Glu-1B x-type-specific PCR fragments. PCR analysis in a wheat cultivar subset carrying different Glu-1B x-type alleles resulted in PCR fragments that differed in size for Glu-B1-1d (B-x6) and non -Glu-B1-1d (B-x6) genotypes. Subsequent sequencing analysis revealed a 15-bp in-frame insertion in the coding regions of all Glu-B1-1d (B-x6) genotypes which allowed the development of a B-x6-specific PCR assay for high-throughput allele sizing by ion-pair reversed-phase high-performance liquid chromatography. The assay was validated in a set of 86 German wheat cultivars, and genotyping data unequivocally verified the presence of HMW glutenin subunits GLU-B1-1D (Bx-6) + GLU-B1-2A (By-8) by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. These results demonstrate that the PCR assay can be applied for the detection and negative selection of the 'poor breadmaking quality' Glu-B1-1d (B-x6) alleles in wheat breeding programs.

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PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat

Cited by 68 publications

References 16 publications

Characterization of agronomic and quality traits and HSW-G5 compositions from the progenies of common wheat (Triticum aestivum L.) with different protein content

Characterization of agronomic and quality traits and HSW-G5 compositions from the progenies of common wheat (Triticum aestivum L.) with different protein content

Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii

Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat

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