PCR-Based National Bacterial Meningitis Surveillance in Turkey

Toprak, Demet; Soysal, Ahmet; Torunoğlu, Mehmet Ali; Turğut, Mehmet; Türkoğlu, Salih; Pimenta, Fabiana Cristina; Carvalho, Maria da Glória; Wang, Xin; Mayer, Leonard W.; Altnkanat, Gülşen; Sõyletır, Gũner; Mete, Bilgül; Bakr, M. I. Abu

doi:10.1097/inf.0000000000000378

Cited by 19 publications

(28 citation statements)

References 11 publications

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“…The pneumococcal isolates were serotyped using the Centers for Disease Control and Prevention (CDC) procedure, and the multiplex PCR reactions were performed according to a published protocol [6,7]. For the eight different multiplex PCR reactions, 40 primer pairs were used to detect the serotypes, as follows: 1, 2, 3, 4, 5, The multiplex PCR reactions were performed using an iCyclerTermal Cycler (Biorad) under the following conditions: 94°C for 4 min of initial denaturation, 30 cycles at 94°C for 45 s, 54°C for 45 s, and 65°C for 2 min and 30 s (see http://www.…”

Section: Multiplex Pcr Reactions For Serotypingmentioning

confidence: 99%

The impact of a pneumococcal conjugate vaccination program on the nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among healthy children in Turkey

Soysal

Karabağ-Yılmaz

Kepenekli

et al. 2016

Vaccine

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Section: Multiplex Pcr Reactions For Serotypingmentioning

confidence: 99%

The impact of a pneumococcal conjugate vaccination program on the nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among healthy children in Turkey

Soysal

Karabağ-Yılmaz

Kepenekli

et al. 2016

Vaccine

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“…‡ References [16,18,19,22] are particularly important studies because of their prospective design, sample size, representativeness and molecular methods used for diagnosis like PCR. § Ratio of deaths associated with meningitis and complications regardless of the involved etiological agent for all cases.…”

mentioning

confidence: 99%

Review of invasive meningococcal disease during the last 40 years in Turkey

Bakır

Altınel²

2015

Expert Review of Vaccines

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Due to the lack of comprehensive surveillance data representing Turkey, the authors aimed to derive information by panoramically reviewing all articles related to invasive meningococcal disease (IMD) published in the last 40 years. The following databases were reviewed: Ulakbim (the national database), BIOSIS Previews (from 1995), Evidence-Based Medicine Reviews-Cochrane Database of Systematic Reviews (from 2005), Embase (from 1996), Ovid MEDLINE(R) (from 1946) and Journals@Ovid Full Text (2014). Twenty-seven articles, 10 published in international journals and 17 in national journals, were identified. Only two were multicenter sentinel meningitis surveillance studies. Also, 74% of IMD patients were aged 5 years or younger and the median overall case fatality rate during childhood was 18.44%. Turkey is a country where meningococcal vaccination on a national basis is recommended by WHO. A vaccination strategy for serogroups B and W135 targeting the first 5 years, covering especially the first 12 months, would be appropriate.

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“…Previous studies investigating invasive meningococcal diseases reported serogroup B as the leading serogroup, especially in European countries and Turkey, in recent years [4,18]. Another study from Turkey reported that serogroup B was the most common cause of meningococcal meningitis in the Marmara region, including the city of Istanbul [5].…”

Section: Discussionmentioning

confidence: 99%

“…In Turkey, the meningococcal vaccines are not implemented in the NIP but are recommended for highrisk groups including Hajj pilgrims and military recruits. Moreover, few surveillance data exist on meningococcal invasive diseases in Turkey [4,5]. During the years 2006 to 2009, we performed national bacterial meningitis surveillance in children and found that N. meningitidis was the second-most common pathogen, and after 2008, due to implementation of Haemophilus influenzae type b vaccine and pneumococcal conjugated vaccine in NIP, N. meningitidis became the leading pathogen in 2008 and 2009 [4].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, few surveillance data exist on meningococcal invasive diseases in Turkey [4,5]. During the years 2006 to 2009, we performed national bacterial meningitis surveillance in children and found that N. meningitidis was the second-most common pathogen, and after 2008, due to implementation of Haemophilus influenzae type b vaccine and pneumococcal conjugated vaccine in NIP, N. meningitidis became the leading pathogen in 2008 and 2009 [4]. During this surveillance, we detected 47 N. meningitidis cases; serogroup B (86%) was the leading serogroup [4].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Neisseria meningitidis carriage: a small-scale survey in Istanbul, Turkey

Kadayıfçı

Merdan

Soysal

et al. 2016

J Infect Dev Ctries

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Introduction: The human nasopharynx is the main reservoir of Neisseria meningitidis, and asymptomatic carriage is common. N. meningitidis one of the common causes of bacterial meningitis in Turkey, especially after the implementation of the national immunization program that includes conjugated pneumococcal and Haemophilus influenzae type b vaccines. The purpose of this study was to evaluate the prevalence of meningococcal carriage and determine the leading serogroup, which may help authorities to adapt appropriate meningococal vaccine into the national immunization programme. Methodology: The prevalence of oropharyngeal carriage of N. meningitidis in 1,000 healthy subjects, 0-79 years of age, was investigated. Oropharyngeal swabs were collected during an 18-month period. Samples obtained were inoculated onto Thayer-Martin agar. The API-NH test and VITEK-MS system were used for identification of colonies. Multiplex real-time polymerase chain reaction assay was used to determine serogroups with serogroup-specific genes. Results: N. meningitidis was isolated from 6 of 1,000 subjects (0.6%). Meningoccocal carriers were between 21 and 40 years of age. All isolates were serogrouped as B, except one that did not survive on subculture. N. lactamica was isolated from 13 of 1,000 subjects (1.3%). Conclusions: Carriage rate of meningococci in our study was relatively low. However, we detected that serogroup B was the leading strain in meningococcal carriage in Istanbul; choosing an appropriate meningococcal vaccine containing serogroup B should therefore be considered. High absolute humidity throughout the year in Istanbul may explain the low prevalence of carriage in our study. This should be verified with a multicenter national survey.

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PCR-Based National Bacterial Meningitis Surveillance in Turkey

Cited by 19 publications

References 11 publications

The impact of a pneumococcal conjugate vaccination program on the nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among healthy children in Turkey

The impact of a pneumococcal conjugate vaccination program on the nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among healthy children in Turkey

Review of invasive meningococcal disease during the last 40 years in Turkey

Prevalence of Neisseria meningitidis carriage: a small-scale survey in Istanbul, Turkey

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