PCR in the diagnosis of cutaneous tuberculosis

Ogusku, Maurício Morishi; Sadahiro, Aya; Hirata, Mário Hiroyuki; Hirata, Rosário Dominguez Crespo; Zaitz, Clarisse; Salem, Júlia Ignez

doi:10.1590/s1517-83822003000200015

Cited by 15 publications

(18 citation statements)

References 17 publications

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“…TB cutis orificialis, a rare manifestation of cutaneous TB (caused by auto-inoculation of M. tuberculosis in patients with advanced internal TB), has been confirmed by PCR (Choi et al, 2009). Using culture/histopathology as the gold standard, IS6110-based conventional PCR/ nested PCR has been well documented in diagnosing cutaneous TB and that showed superiority over 16S rRNA gene-based PCR (Ogusku et al, 2003;Obieta et al, 2010). A highly sensitive and specific PCR assay targeting 65 kDa protein gene has also been developed for the diagnosis of cutaneous TB, considering culture/response to ATT as the gold standard (Negi et al, 2005a;Abdalla et al, 2009).…”

Section: Cutaneous Tuberculosismentioning

confidence: 99%

“…Interestingly, Okazaki et al (2005) reported first case of M. bovis BCG-derived cutaneous TB (localized at different area from the vaccination site) without immune deficiency by multiplex PCR assay based on region of difference (RD) 1, complement sequence of RD1, RD2, RD8, RD14 and SenX3-RegX3 regions originating from M. bovis BCG Tokyo 172. Using culture/histopathology as the gold standard, IS6110-based conventional PCR/ nested PCR has been well documented in diagnosing cutaneous TB and that showed superiority over 16S rRNA gene-based PCR (Ogusku et al, 2003;Obieta et al, 2010). Using culture/histopathology as the gold standard, IS6110-based conventional PCR/ nested PCR has been well documented in diagnosing cutaneous TB and that showed superiority over 16S rRNA gene-based PCR (Ogusku et al, 2003;Obieta et al, 2010).…”

Section: Cutaneous Tuberculosismentioning

confidence: 99%

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Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

160

134

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During the last two decades, the resurgence of tuberculosis (TB) has been documented in both developed and developing nations, and much of this increase in TB burden coincided with human immunodeficiency virus (HIV) epidemics. Since then, the disease pattern has changed with a higher incidence of extrapulmonary tuberculosis (EPTB) as well as disseminated TB. EPTB cases include TB lymphadenitis, pleural TB, TB meningitis, osteoarticular TB, genitourinary TB, abdominal TB, cutaneous TB, ocular TB, TB pericarditis and breast TB, although any organ can be involved. Diagnosis of EPTB can be baffling, compelling a high index of suspicion owing to paucibacillary load in the biological specimens. A negative smear for acid-fast bacilli, lack of granulomas on histopathology and failure to culture Mycobacterium tuberculosis do not exclude the diagnosis of EPTB. Novel diagnostic modalities such as nucleic acid amplification (NAA) can be useful in varied forms of EPTB. This review is primarily focused on the diagnosis of several clinical forms of EPTB by polymerase chain reaction (PCR) using different gene targets.

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Section: Cutaneous Tuberculosismentioning

confidence: 99%

Section: Cutaneous Tuberculosismentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

160

134

View full text Add to dashboard Cite

show abstract

“…The cutaneous tuberculosis can occur by direct penetration of M. tuberculosis in the dermis (primary infection) or by dissemination of the same, beginning with a pulmonary focus (secondary infections). [6] Cutaneous tuberculosis and atypical mycobacteria skin infection (AMI) present a wide range of clinical manifestations, varying from warty, nodules and papulonecrotic lesions, to ulcerations and abscesses. [7] In a study from north India Lupus vulgaris (LV) was the most frequent manifestation (55%), followed by scrofuloderma (SFD) (27%), TB verrucosa cutis (TBVC) (6%), tuberculous gumma (5%), and tuberculids (7%).…”

Section: Introductionmentioning

confidence: 99%

Comparison of conventional and molecular methods in diagnosis of extrapulmonary (cutaneous) tuberculosis in a tertiary care hospital in Delhi

Lall¹,

Singh²,

Chaudhary³

et al. 2017

Int J Med Sci Public Health

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Background: Tuberculosis (TB) is a potentially fatal contagious disease caused by Mycobacterium tuberculosis. The one third of the world's population is infected with tubercle bacilli. According to World Health Organisation (WHO), global incidence of TB in 2014 was 9 million cases out of which 2.2 million cases were of India only. India has the highest burden of TB. Extrapulmonary Tuberculosis (EPTB) constitutes about 15-20% of TB cases. Cutaneous TB constitutes about 1.5% of all EPTB cases. The incidence of cutaneous TB amongst total dermatology patients varies between 0.1% and 2% in different studies. Objective: To compare the conventional and molecular methods in diagnosis of extrapulmonary (cutaneous) tuberculosis. Materials and methods: A cross-sectional study was conducted on consecutive patients of cutaneous tuberculosis in the departments of Microbiology and Dermatology at UCMS & Guru Teg Bahadur Hospital Delhi, from all sample received during the time period between Nov 2010 and March 2012. Conventional method of diagnosis of tuberculosis was performed on biopsy samples i.e. staining and culture methods. The conventional methods of diagnosis are then compared with molecular methods PCR targeting 16S rRNA. The two set of primers were used. The outer (16 SOL,16 SOR) and inner pairs (16 SIL,16 SIR) of primers are expected to be the genus specific and species specific primers for 16S rRNA gene amplification, respectively. Result: 31 samples received during the time period between November 2010 and March 2012. Female predominance was found in present study 54.8% (17). The clinical types of the received samples from cutaneous tuberculosis patients includes ,TBVC (6.4%), SFD (25.8%) and LV (67.7%). Fifty eight percent of patients were found to be in age group 11-20 years. Ziehl-Neelsen (ZN) staining was performed on smears of all biopsy specimens. The 6.4% (2) were ZN stain positive and 12% (4) were Auramine stain positive and 6 (19.3) were culture positive. Nested PCR was performed on 31 biopsy specimens. Eight (25.8%) specimens were found to be positive for common Mycobacterium species. Out of 8, DNA from 6 biopsy specimens were amplified by both genus specific and species specific primers based on 16S rRNA gene amplification. They were diagnosed as M. tuberculosis infection. Conclusion: PCR tests offer alternative robust approach to detect M. tuberculosis in paucibacillary EPTB specimens that show rapid results with good diagnostic accuracy. Although, these tests cannot replace the conventional AFB smear, culture identification or histopathological observations but they contribute significantly for an early diagnosis of EPTB and exert an acceptable impact on the clinical management of disease.

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“…The most extensively used molecular epidemiology technique is Restriction Fragment Length Polymorphism (RFLP) typing, which uses the insertion sequence IS6110 to differentiate clinical isolates (5,37). Polymerase Chain Reaction (PCR) is the most sensitive method in the diagnosis of clinically suspected tuberculosis (1,8,25). New typing methods based on the PCR, such as spoligotyping (18), and mycobacterial interspersed repetitive units (MIRU) typing have also been described (34).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method

Ramazanzadeh

Farnia

Amirmozafari³

2009

Braz. J. Microbiol.

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Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these spoligotype isolates were unique and reported for the first time. The remaining18 (12.8%) spoligotype patterns were previously reported from other geographical regions of the world. Haarlem family was most prevalent than other genotype. Antibiotic resistances were higher in those isolated from the Iranian patients. The pcaA gene was detected in M. tuberculosis clinical isolates but not in saprophyte strains such as M. kansasi. The results showed that, spread of M. tuberculosis strains belonging to the Beijing family among Iranian patients has to be considered seriously. This study confirmed the widespread existence of pcaA gene in almost all the clinical isolates. It is also important to undertake studies to identify which factors are the most significant to consider in tuberculosis control program.

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PCR in the diagnosis of cutaneous tuberculosis

Cited by 15 publications

References 17 publications

Diagnosis of extrapulmonary tuberculosis by PCR

Diagnosis of extrapulmonary tuberculosis by PCR

Comparison of conventional and molecular methods in diagnosis of extrapulmonary (cutaneous) tuberculosis in a tertiary care hospital in Delhi

Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method

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