PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes

Diguță, Camelia; Vincent, Béatrice; Guilloux-Bénatier, Michèle; Alexandre, Hervé; Rousseaux, Sandrine

doi:10.1016/j.fm.2011.03.006

Cited by 57 publications

(48 citation statements)

References 50 publications

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“…Furthermore, some cultivars show high susceptibility to aspergillus rot as a result of intrinsic genetic features and bunch conformation. Penicillium commune is another filamentous fungus that is associated with grape decay, even if not much has been reported . This fungus is commonly found in vineyards in Jiangsu Province, China.…”

Section: Introductionmentioning

confidence: 99%

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

et al. 2018

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“…The presence of Aureobasidium on grapes has been previously well reported (Barata et al 2012;Duncan et al 1995) and, like Epicoccum, the species A. pullulans is the only one cited (Diguta et al 2011;Pancher et al 2012;Rousseaux et al 2014). This species shows considerable morphological and physiological variability and different varieties have been described (Zalar et al 2008).…”

Section: Discussionmentioning

confidence: 76%

“…The occurrence of Epicoccum on grapevine and grape as epiphytic, endophytic and/or pathogenic is well documented (Duncan et al 1995;Rousseaux et al 2014), but the only species mentioned is E. nigrum (Diguta et al 2011;Pancher et al 2012). Questions related to Phoma complex taxonomy are still unanswered and Aveskamp et al (2010) described other Epicoccum species (i.e.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration

Lorenzini

Zapparoli

2015

Antonie van Leeuwenhoek

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Fungi like Cladosporium, Fusarium, Epicoccum and Aureobasidium can occur on withered grapes causing spoilage of passito wine. There is little or no information on the pathogenic role of these fungi. This study describes the isolation, incidence and identification of several isolates from different withered rotten grapes. Representative isolates grouped in several phenotypes were identified by phylogenetic analysis of internal transcribed spacer, actin or elongation factor gene sequences. Isolates of Cladosporium and Fusarium were ascribed to different species, of these C. ramotenellum, C. halotolerans and F. graminearum were isolated from Vitis vinifera for the first time. All Epicoccum and Aureobasidium isolates belonged to E. nigrum and A. pullulans, respectively. Random amplified DNA polymorphism analysis showed high level of heterogenicity among Epicoccum and Fusarium isolates. Infection assays were carried out to evaluate infectivity in some strains under different withering conditions. Fusarium spp. strains had similar infectivity, while significant variability was observed among Cladosporium spp. and E. nigrum strains. A. pullulans resulted particularly infective. This study provided insights into the occurrence and infection of these fungi in fruit-drying rooms with important implications towards control management during the withering.

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“…Morphological studies can provide information to identify Trichoderma species, but it is insufficient because Trichoderma species have relatively few morphological characters and limited disparity, which may cause interference and misidentification [33]. Furthermore, cultural conditions also can affect morphological characteristics [34].…”

Section: Morphological Identification Of Trichoderma Sppmentioning

confidence: 99%

Induced Systemic Resistance in Two Genotypes of Brassica napus (AACC) and Raphanus oleracea (RRCC) by Trichoderma Isolates against Sclerotinia sclerotiorum

Alkooranee¹,

Aledan²,

Xiang³

et al. 2015

AJPS

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Two different species, Trichoderma viride TV10 and Trichoderma harzianum TH12 from 30 Trichoderma isolates were selected out based on their high growth inhibition of the phytopathogen Sclerotinia sclerotiorum (Lib) de Bary, which reached 84.44% and 100%, respectively. Their untreated culture filtrates (CF) and culture filtrates treated with heat (CFH) also were tested for growth inhibition of the pathogen in potato dextrose agar (PDA). Morphological and molecular characterisation by internal transcribed spacer (ITS) PCR provided consistent identification of these isolates. The degree of infection and disease index (DI) of S. sclerotiorum were examined in Brassica napus (AACC) and Raphanus alboglabra (RR) and Brassica alboglabra (CC). The results revealed that Raphanus alboglabra showed higher disease resistance than that of B. napus. Biotic elecitors T. harzianum TH12 and T. viride TV10 and their CF and CFH demonstrated the ability to cause induced systemic resistance (ISR) in B. napus and Raphanus alboglabra against sclerotinia stem rot (SSR) disease. Furthermore, a high ability to reduce the degree of infection and DI in B. napus with the biotic elicitors T. harzianum TH12 and T. viride TV10 was observed, with numbers reaching 7.22% to 6.67% and 17.78% to 11.67%, respectively. When CF were used, reached 20.00% to 16.67% and 33.33% to 23.33%, respectively; with CFH, values reached 35.00% to 21.67% and 37.78% to 28.33%, respectively. While in Raphanus alboglabra the degree of infection and DI * Corresponding authors. J. T. Alkooranee et al. 1663reached 0.00% and 0.00% with all biotic elicitors treatments. These results show that biotic elicitor treatments significantly (P < 0.05) can increase the fresh and dry weights of both roots and shoots as well as plant height compared with controls. The TH12 treatment in B. napus and Raphanus alboglabra ranked as most effective. This study showed for the first time the ability of genotype Raphanus alboglabra (RRCC) to demonstrate resistance against S. sclerotiorum with or without treatment by biotic elicitors and the ability of genotype B. napus (AACC) to demonstrate resistance to the pathogen after treatment with biotic elicitors.

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PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes

Cited by 57 publications

References 50 publications

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration

Induced Systemic Resistance in Two Genotypes of <i>Brassica napus</i> (AACC) and <i>Raphanus oleracea</i> (RRCC) by <i>Trichoderma</i> Isolates against <i>Sclerotinia sclerotiorum</i>

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PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes

Cited by 57 publications

References 50 publications

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

Exogenous trehalose enhanced the biocontrol efficacy of Hanseniaspora uvarum against grape berry rots caused by Aspergillus tubingensis and Penicillium commune

Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration

Induced Systemic Resistance in Two Genotypes of &lt;i&gt;Brassica napus&lt;/i&gt; (AACC) and &lt;i&gt;Raphanus oleracea&lt;/i&gt; (RRCC) by &lt;i&gt;Trichoderma&lt;/i&gt; Isolates against &lt;i&gt;Sclerotinia sclerotiorum&lt;/i&gt;

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Induced Systemic Resistance in Two Genotypes of <i>Brassica napus</i> (AACC) and <i>Raphanus oleracea</i> (RRCC) by <i>Trichoderma</i> Isolates against <i>Sclerotinia sclerotiorum</i>