PDGF and VEGF Levels in Platelet-Rich Plasma 

Abstract

PDGF and VEGF are two of the most potent mitogen for connective tissue, its secretion appears to be particularly important when the source is Platelet Rich Plasma (PRP), hence the latter leading role in tissue regeneration. ELISA PDGFBB levels in PRP, Platelet Poor Plasma (PPP) and exudates, were determined in 32 healthy subjects before and 24 hours after ingestion of Aspirin (ASA) and Clopidogrel (CLO). Results: PDGFBB baseline levels were 10.6 ± 1.9 ng / ml (PPP), 12.12 ± 2.5 ng / ml (PRP) and 10.84 ± 1.68 ng / ml (exudate) While after treatment with PDGFBB ASA concentrations were at 8.96 ± 1.4 ng / ml (PPP), 11.36 ± 1.48 ng / ml (PRP), 11.11 ± 1.14 ng / ml (exudate) and the Clopidogrel were 8.53 ± 0.59 ng / ml (PPP), 9.65 ± 1.17 ng / ml (PRP) and 8.51 ± 0.75 ng / ml (exudate). VEGF basal values were 973.9 ± 590.3 pg / ml (PPP), 1184.2 ± 288.4 pg / ml (PRP), 1069.3 ± 192.3 pg / ml (exudate). After treatment with ASA VEGF values were at 1439.5 ± 117.4 pg / ml (PPP), 1802.3 ± 123.9 pg / ml (PRP), 1745.6 ± 171.6 pg / ml (exudate) and the Clopidogrel were 577.8 ± 150.6 pg / ml (PPP), 668.7 ± 100.6 pg / ml (PRP), 624.9 ± 106.7 pg / ml (exudate). Conclusions: It was noted that after administration of antiplatelet drugs PDGFBB and VEGF values decreased statistically significantly, especially for the group of Clopidogrel. The ASA lesser extent appear to affect the concentrations of both factors, which may be attributable to the pharmacological action mechanism between the ASA and Clopidogrel.

Osteogenesis Capability of Gelatin-based biomimetic Scaffold containing PRP-loaded Starch Nanoparticles

“…The initial explosive release was observed in both growth factors, with about 50% of the total growth factors measured during the experiment released during the rst day. Release kinetics of both growth factors follow the same pattern, and of course, PDGF has a higher concentration than VEGF, which is consistent with the fact that the amount of VEGF in PRP is lower than in PDGF [40]. The important point is that in the scaffold preparation stages, steps such as sterilization and cross-linking, the initial explosive release is partially removed from the system and a more stable system is achieved.…”

In vivo study of wound healing processes in Sprague-Dawley model using human mesenchymal stem cells and platelet-rich plasma

“…The effectiveness of MSCs to promote wound healing has also been proven in xenogenic applications [16][17][18][19][20] . Another well-known modality to improve the wound healing process is platelet-rich plasma (PRP) which contains various growth factors that facilitate the wound healing process [21][22][23][24][25] . PRP has shown excellent results in many in vivo studies as well as in vitro studies related to wound healing 21,26-32 .…”

Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma

“…Platelet rich plasma (PRP) is a generic term referring to any sample of autologous or allogeneic plasma with platelets concentrations above baseline blood values. PRP has been known as a powerful adhesive and hemostatic agent since the 1970s and as a rich source of autologous growth factors since the 1990s [ 1 , 2 ].…”