PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection

Ramón-Carbonell, Marta de; López‐Pérez, Mario; González-Candelas, Luı́s; Sánchez-Torres, Paloma

doi:10.3390/jof5040100

Cited by 33 publications

(34 citation statements)

References 46 publications

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“…The MFS is a class of transport proteins that plays an important role in many substrate transports in cells, and it can pump off the fungal toxins, thereby enhance the ability of P. digitatum to infect the plant host. In addition, the overexpression of genes encoding MFS transporter conferred the drug-resistance of fungi ( Wu et al, 2016 ; de Ramón-Carbonell et al, 2019 ). In this work, the genes encoding MFS monosaccharide (26236839), sugar (26228725), peptide (26233853) transporter were downregulated by 2.03, 1.48, and 1.82-fold, respectively.…”

Section: Discussionmentioning

confidence: 99%

Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum

Feng

Wang

et al. 2020

Front. Microbiol.

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Penicillium digitatum is the most damaging pathogen provoking green mold in citrus fruit during storage, and there is an urgent need for novel antifungal agents with high efficiency. The aim of this study was to investigate the antifungal effects of peptide thanatin against P. digitatum and the molecular mechanisms. Results showed that peptide thanatin had a prominent inhibitory effect on P. digitatum by in vitro and in vivo test. A total of 938 genes, including 556 downregulated and 382 upregulated genes, were differentially expressed, as revealed by RNA-seq of whole P. digitatum genomes analysis with or without thanatin treatment. The downregulated genes mainly encoded RNA polymerase, ribosome biogenesis, amino acid metabolism, and major facilitator superfamily. The genes associated with heat shock proteins and antioxidative systems were widely expressed in thanatin-treated group. DNA, RNA, and the protein content of P. digitatum were significantly decreased after thanatin treatment. In conclusion, thanatin could inhibit the growth of P. digitatum, and the underlying mechanism might be the genetic information processing and stress response were affected. The research will provide more precise and directional clues to explore the inhibitory mechanism of thanatin on growth of P. digitatum.

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Section: Discussionmentioning

confidence: 99%

Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum

Feng

Wang

et al. 2020

Front. Microbiol.

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“…These membrane-bound proteins are known to provide protection against a wide range of naturally occurring and xenobiotic toxic compounds [ 98 ]. Many studies have reported links between enhanced efflux transporter activity and the appearance of resistance in different fungal pathogens [ 41 , 99 , 100 , 101 , 102 ] including Pd, indicating that efflux transporters may have a common and critical role in fungicide sensitivity. In addition, coincident resistance to many chemical types of fungicides was found to be attributable to overexpression of efflux pumps in some important fungal pathogens.…”

Section: Resistance-mediated Drug Efflux Transportersmentioning

confidence: 99%

Molecular Mechanisms Underlying Fungicide Resistance in Citrus Postharvest Green Mold

Sánchez-Torres

2021

JoF

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The necrotrophic fungus Penicillium digitatum (Pd) is responsible for the green mold disease that occurs during postharvest of citrus and causes enormous economic losses around the world. Fungicides remain the main method used to control postharvest green mold in citrus fruit storage despite numerous occurrences of resistance to them. Hence, it is necessary to find new and more effective strategies to control this type of disease. This involves delving into the molecular mechanisms underlying the appearance of resistance to fungicides during the plant–pathogen interaction. Although mechanisms involved in resistance to fungicides have been studied for many years, there have now been great advances in the molecular aspects that drive fungicide resistance, which facilitates the design of new means to control green mold. A wide review allows the mechanisms underlying fungicide resistance in Pd to be unveiled, taking into account not only the chemical nature of the compounds and their target of action but also the general mechanism that could contribute to resistance to others compounds to generate what we call multidrug resistance (MDR) phenotypes. In this context, fungal transporters seem to play a relevant role, and their mode of action may be controlled along with other processes of interest, such as oxidative stress and fungal pathogenicity. Thus, the mechanisms for acquisition of resistance to fungicides seem to be part of a complex framework involving aspects of response to stress and processes of fungal virulence.

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“…These resistance mechanisms have been explained for P. digitatum , P. expansum , and Monilinia fructicola , among others (Ali & Amiri, 2018; de Ramón‐Carbonell & Sánchez‐Torres, 2020; Hamamoto et al., 2000; Luo & Schnabel, 2008; Wang et al., 2014). A typical case is the new major facilitator superfamily transporter PdMFS1 of P. digitatum , which was recently recognized as being involved in the process of acquiring resistance against fungicides (de Ramón‐Carbonell et al., 2019). Consequently, there has been a growing interest in environmentally friendly approaches.…”

Section: Recent Strategies For Securing Fruit Productionmentioning

confidence: 99%

Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections

Ngea

Qian

Yang

et al. 2021

Comp Rev Food Sci Food Safe

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Fruit‐based diets have been adopted by the public worldwide because of their nutritional value. Many advances have also been made in the elucidation of host–pathogen interaction in the postharvest phase of fruits, in the hope of improving the management of diseases caused by pathogenic molds. In this study, we presented the molecular mechanisms by which pathogenic mold infects fruit in the postharvest phase, and focused on the knowledge gained from recent molecular techniques such as differential analysis of gene expression, targeted insertion, and mutagenesis. Current postharvest pathogenic fungal control strategies were then examined on the basis of their mechanisms for altering the infection process in order to explore new perspectives for securing fruit production. We found that biotechnological advances have led to an understanding of the new basic molecular processes involved in fruit fungal infection and to the identification of new genes, proteins and key factors that could serve as ideal targets for innovative antifungal strategies. In addition, the most commonly used steps to evaluate an approach to disrupt the fruit fungal infection process are mainly based on the inhibition of mycelial growth, spore germination, disruption of Adenosine triphosphate (ATP) synthesis, induction of oxidative stress, cell wall membrane damage, and inhibition of key enzymes. Finally, the alteration of the molecular mechanisms of signaling and response pathways to infection stimulation should also guide the development of effective control strategies to ensure fruit production.

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PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection

Cited by 33 publications

References 46 publications

Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum

Effects of Peptide Thanatin on the Growth and Transcriptome of Penicillium digitatum

Molecular Mechanisms Underlying Fungicide Resistance in Citrus Postharvest Green Mold

Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections

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